Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1982 Feb;45(2):365–370.

Isolation of Clq-binding virus-antibody immune complexes from lactic dehydrogenase virus (LDV)-infected mice.

T L McDonald
PMCID: PMC1555263  PMID: 6977483

Abstract

A Sepharose-IgG-Clq sorbent has been successfully used to isolate circulating virus-antibody (virus Ab) complexes from serum of lactic dehydrogenase virus (LDV)-infected mice. The chronological study demonstrated that although circulating LDV in persistently infected mice was complexed with IgG antibody, the virus-Ab complexes bound to the Clq sorbent only early during the infection. Inactivation of these Clq-isolated virus Ab complexes was accomplished with rabbit anti-mouse IgG but not anti-IgM. The critical time period for the demonstration of Clq-binding LDV-Ab complexes in serum was between 10 and 18 days postinoculation. The reasons for the apparent shift from Clq-binding virus-Ab complexes to non-Clq binding are not clear, however, possible explanations and implications in immune complex tissue injury are discussed.

Full text

PDF
365

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cuatrecasas P., Wilchek M., Anfinsen C. B. Selective enzyme purification by affinity chromatography. Proc Natl Acad Sci U S A. 1968 Oct;61(2):636–643. doi: 10.1073/pnas.61.2.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hellström K. E., Hellström I., Nepom J. T. Specific blocking factors--are they important? Biochim Biophys Acta. 1977 Dec 23;473(2):121–148. doi: 10.1016/0304-419x(77)90003-8. [DOI] [PubMed] [Google Scholar]
  3. Kauffmann R. H., Van Es L. A., Daha M. R. Aggregated human immunoglobulin G stabilized by albumin: a standard for immune complex detection. J Immunol Methods. 1979;31(1-2):11–22. doi: 10.1016/0022-1759(79)90281-3. [DOI] [PubMed] [Google Scholar]
  4. McDonald T. L., Burger D. Purification of the subunit Clq from the first component of equine complement. Immunology. 1979 Jul;37(3):517–527. [PMC free article] [PubMed] [Google Scholar]
  5. Michaelides M. C., Schlesinger S. Effect of acute or chronic infection with lactic dehydrogenase virus (LDV) on the susceptibility of mice to plasmacytoma MOPC-315. J Immunol. 1974 Apr;112(4):1560–1564. [PubMed] [Google Scholar]
  6. NOTKINS A. L., SHOCHAT S. J. Studies on the multiplication and the properties of the lactic dehydrogenase agent. J Exp Med. 1963 May 1;117:735–747. doi: 10.1084/jem.117.5.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Notkins A. L., Mage M., Ashe W. K., Mahar S. Neutralization of sensitized lactic dehydrogenase virus by anti-gammglobulin. J Immunol. 1968 Feb;100(2):314–320. [PubMed] [Google Scholar]
  8. Notkins A. L., Mahar S., Scheele C., Goffman J. Infectious virus-antibody complex in the blood of chronically infected mice. J Exp Med. 1966 Jul 1;124(1):81–97. doi: 10.1084/jem.124.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Oldstone M. B., Buchmeier M. J., Doyle M. V., Tishon A. Virus-induced immune complex disease: specific anti-viral antibody and C1q binding material in the circulation during persistent lymphocytic choriomeningitis virus infection. J Immunol. 1980 Feb;124(2):831–838. [PubMed] [Google Scholar]
  10. Oldstone M. B., Dixon F. J. Inhibition of antibodies to nuclear antigen and to DNA in New Zealand mice infected with lactate dehydrogenase virus. Science. 1972 Feb 18;175(4023):784–786. doi: 10.1126/science.175.4023.784. [DOI] [PubMed] [Google Scholar]
  11. Oldstone M. B., Dixon F. J. Lactic dehydrogenase virus-induced immune complex type of glomerulonephritis. J Immunol. 1971 May;106(5):1260–1263. [PubMed] [Google Scholar]
  12. Oldstone M. B., Dixon F. J. Pathogenesis of chronic disease associated with persistent lymphocytic choriomeningitis viral infection. I. Relationship of antibody production to disease in neonatally infected mice. J Exp Med. 1969 Mar 1;129(3):483–505. doi: 10.1084/jem.129.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Porter D. D., Porter H. G. Deposition of immune complexes in the kidneys of mice infected with lactic dehydrogenase virus. J Immunol. 1971 May;106(5):1264–1266. [PubMed] [Google Scholar]
  14. Riley V., Lilly F., Huerto E., Bardell D. Transmissible Agent Associated with 26 Types of Experimental Mouse Neoplasms. Science. 1960 Aug 26;132(3426):545–547. doi: 10.1126/science.132.3426.545. [DOI] [PubMed] [Google Scholar]
  15. Sjögren H. O., Hellström I., Bansal S. C., Hellström K. E. Suggestive evidence that the "blocking antibodies" of tumor-bearing individuals may be antigen--antibody complexes. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1372–1375. doi: 10.1073/pnas.68.6.1372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. StC Sinclair N. R. Mechanisms of immunoregulation. Immunoregulation by antibody and antigen-antibody complexes. Transplant Proc. 1978 Jun;10(2):349–353. [PubMed] [Google Scholar]
  17. Turner W., Ebert P. S., Spahn G., Bassin R., Chirigos M. A. Potentiation of murine sarcoma virus (Harvey) (Moloney) oncogenicity in lactic dehydrogenase-elevating virus-infected mice. Proc Soc Exp Biol Med. 1971 Apr;136(4):1314–1318. doi: 10.3181/00379727-136-35482. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES