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. 1982 Feb;45(2):193–198.

Antigen-induced arthritis: inflammation and antigen handling after two different doses of intra-articularly injected antigen.

H J van Beusekom, L B van de Putte, W B van den Berg, W J van den Broek
PMCID: PMC1555287  PMID: 6277772

Abstract

We studied (i) the inflammatory response induced by intra-articular (i.a.) injection of 2.5 and 0.5 mg 125I-labelled bovine serum albumin (125I-BSA) into the paired knee joints of rabbits immunized with BSA in Freund's complete adjuvant, and (ii) handling of these different doses of antigen by means of regular external radioactivity measurements. The joint inflammation was quantified by 99mTechnetium pertechnetate uptake measurements. More severe arthritis was seen in the 2.5 mg BSA-injected than in the paired 0.5 mg BSA-injected knee, both in the early and in the late phase of antigen-induced arthritis. External radioactivity measurements showed enhanced disappearance of radioactivity from the 2.5 mg 125I-BSA challenged knee joint, resulting in lower percentual retention of the dose injected than in the 0.5 mg injected knee joint. However the calculated absolute amount of long term retained BSA in the 2.5 mg BSA injected knee, 4 weeks after i.a. injection, was still about 2.8 times the quantity of BSA retained in the 0.5 mg BSA-injected paired knee joint. These data indicate that the severity of both the early and late phase of antigen-induced arthritis is i.a. antigen dose-dependent and, in addition, suggest that handling of i.a. antigen depends in part on the severity of joint inflammation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baer H., Kolb R. W. Suppression og the tuberculin reaction by high concentrations of tuberculin and the relationship of this phenomenon to the potency assay of tuberculin. J Immunol. 1967 May;98(5):1015–1019. [PubMed] [Google Scholar]
  2. Consden R., Doble A., Glynn L. E., Nind A. P. Production of a chronic arthritis with ovalbumin. Its retention in the rabbit knee joint. Ann Rheum Dis. 1971 May;30(3):307–315. doi: 10.1136/ard.30.3.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cooke T. D., Hurd E. R., Ziff M., Jasin H. E. The pathogenesis of chronic inflammation in experimental antigen-induced arthritis. II. Preferential localization of antigen-antibody complexes to collagenous tissues. J Exp Med. 1972 Feb 1;135(2):323–338. doi: 10.1084/jem.135.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cooke T. D., Jasin H. E. The pathogenesis of chronic inflammation in experimental antigen-induced arthritis. I. The role of antigen on the local immune response. Arthritis Rheum. 1972 Jul-Aug;15(4):327–337. doi: 10.1002/art.1780150402. [DOI] [PubMed] [Google Scholar]
  5. DUMONDE D. C., GLYNN L. E. The production of arthritis in rabbits by an immunological reaction to fibrin. Br J Exp Pathol. 1962 Aug;43:373–383. [PMC free article] [PubMed] [Google Scholar]
  6. Glynn L. E. The chronicity of inflammation and its significance in rheumatoid arthritis. Ann Rheum Dis. 1968 Mar;27(2):105–121. doi: 10.1136/ard.27.2.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goldberg V. M., Lance E. M., Davis P. Experimental immune synovitis in the rabbit. Relative roles of cell mediated and humoral immunity. Arthritis Rheum. 1974 Nov-Dec;17(6):993–1005. doi: 10.1002/art.1780170612. [DOI] [PubMed] [Google Scholar]
  8. Goldlust M. B., Rich L. C., Brown W. R. Immune synovitis in rabbits. Effects of differing schedules for intra-articular challenge with antigen. Am J Pathol. 1978 May;91(2):329–344. [PMC free article] [PubMed] [Google Scholar]
  9. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  10. Hollister J. R., Mannik M. Antigen retention in joint tissues in antigen-induced synovitis. Clin Exp Immunol. 1974 Apr;16(4):615–627. [PMC free article] [PubMed] [Google Scholar]
  11. Jasin H. E. Mechanism of trapping of immune complexes in joint collagenous tissues. Clin Exp Immunol. 1975 Dec;22(3):473–485. [PMC free article] [PubMed] [Google Scholar]
  12. Jokipii A. M., Jokipii L. Dose response relationships and interference of simultaneous skin tests in delayed hypersensitivity. J Clin Pathol. 1973 Sep;26(9):687–690. doi: 10.1136/jcp.26.9.687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Menard H. A., Dion J. Experimental immune arthritis: host factors. J Rheumatol. 1975 Dec;2(4):373–383. [PubMed] [Google Scholar]
  14. Ménard H. A., Dion J. Is local antigen persistence responsible for the chronicity of the experimental immune arthritis of the rabbit. Experientia. 1976 Nov 15;32(11):1474–1475. doi: 10.1007/BF01937443. [DOI] [PubMed] [Google Scholar]
  15. Sweet G. H., Welborn F. L. Use of chromium chloride as the coupling agent in a modified plaque assay. Cells producing anti-protein antibody. J Immunol. 1971 May;106(5):1407–1410. [PubMed] [Google Scholar]
  16. Von Blomberg M., Boerrigter G. H., Scheper R. J. Interference of simultaneous skin tests in delayed hypersensitivity. Immunology. 1978 Aug;35(2):361–367. [PMC free article] [PubMed] [Google Scholar]
  17. Webb F. W., Ford P. M., Glynn L. E. Persistence of antigen in rabbit synovial membrane. Br J Exp Pathol. 1971 Feb;52(1):31–35. [PMC free article] [PubMed] [Google Scholar]
  18. van Beusekom H. J., van de Putte L. B., van den Berg W. B., van den Broek W. J., Buijs W. C. Antigen handling in antigen-induced joint inflammation: kinetics of a second intra-articularly injected dose of antigen in an already established antigen-induced joint inflammation. Immunology. 1981 Sep;44(1):153–161. [PMC free article] [PubMed] [Google Scholar]
  19. van Beusekom H. J., van den Broek W. J., van de Putte L. B., Buys W. C., van den Berg W. B. 99mTc-pertechnetate uptake measurement in the rabbit knee-joint. J Rheumatol. 1981 Jan-Feb;8(1):31–39. [PubMed] [Google Scholar]

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