Skip to main content
Immunology logoLink to Immunology
. 1982 Jul;46(3):653–659.

Anti-idiotype regulation of timothy grass pollen IgE antibody formation. I. In vivo induction of suppressor T cells.

A Malley, D W Dresser
PMCID: PMC1555294  PMID: 6212537

Abstract

Antibodies prepared against antigen B-specific T-helper factor (anti-THF) and antigen B-specific IgE (anti-IgEid) have been isolated and the anti-idiotypic properties of both antibodies were described previously. Both anti-idiotypic antibodies (anti-Id) were used to determine their influence in primary and secondary IgE responses. These studies demonstrated that both anti-Id specifically suppress timothy IgE responses, that the suppression lasts for at least 35 days, and that it is mediated by a Thy 1+ Lyt 1- 23+ T cell. Attempts to induce suppressor T cells with the F(ab)2 fragment of anti-THF at several concentrations (10-80 micrograms per day for 3 successive days) were unsuccessful. This suggests that the Fc portion may be important in presentation of anti-Id during the induction of suppressor T cells.

Full text

PDF
653

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binz H., Frischknecht H., Shen F. W., Wigzell H. Idiotypic determinants on T-cell subpopulations. J Exp Med. 1979 Apr 1;149(4):910–922. doi: 10.1084/jem.149.4.910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Black S. J., Herzenberg L. A. B-cell influences on the induction of allotype suppressor T cells. J Exp Med. 1979 Jul 1;150(1):174–183. doi: 10.1084/jem.150.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brandt C. J., Deppe L. B., Malley A. Antigen B-specific helper factor from timothy grass pollen: isolation and partial characterization. Immunology. 1981 Oct;44(2):373–379. [PMC free article] [PubMed] [Google Scholar]
  4. Chayen A., Parkhouse R. M. Preparation and properties of a cytotoxic monoclonal rat anti-mouse Thy-1 antibody. J Immunol Methods. 1982;49(1):17–23. doi: 10.1016/0022-1759(82)90362-3. [DOI] [PubMed] [Google Scholar]
  5. Cosenza H., Köhler H. Specific suppression of the antibody response by antibodies to receptors. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2701–2705. doi: 10.1073/pnas.69.9.2701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eichmann K. Expression and function of idiotypes of lymphocytes. Adv Immunol. 1978;26:195–254. doi: 10.1016/s0065-2776(08)60231-x. [DOI] [PubMed] [Google Scholar]
  7. Fairchild S. S., Malley A. Induction of mouse homocytotropic antibodies to Timothy pollen antigens. J Immunol. 1975 Aug;115(2):446–449. [PubMed] [Google Scholar]
  8. Fitch F. W. Selective suppression of immune responses. Regulation of antibody formation and cell-mediated immunity by antibody. Prog Allergy. 1975;19:195–244. [PubMed] [Google Scholar]
  9. Hart D. A., Wang A. L., Pawlak L. L., Nisonoff A. Suppression of idiotypic specificities in adult mice by administration of antiidiotypic antibody. J Exp Med. 1972 Jun 1;135(6):1293–1300. doi: 10.1084/jem.135.6.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hetzelberger D., Eichmann K. Idiotype suppression. III. Induction of unresponsiveness to sensitization with anti-idiotypic antibody: identification of the cell types tolerized in high zone and in low zone, suppressor cell-mediated, idiotype suppression. Eur J Immunol. 1978 Dec;8(12):839–846. doi: 10.1002/eji.1830081204. [DOI] [PubMed] [Google Scholar]
  11. Jerne N. K. Towards a network theory of the immune system. Ann Immunol (Paris) 1974 Jan;125C(1-2):373–389. [PubMed] [Google Scholar]
  12. Malley A., Begley D., Forsham A. Chemical modification of timothy grass pollen antigen B--I. Loss of antibody binding properties. Mol Immunol. 1979 Nov;16(11):929–934. doi: 10.1016/0161-5890(79)90093-2. [DOI] [PubMed] [Google Scholar]
  13. Malley A., Begley D., Forsham A. Preparation of high titered rabbit anti-mouse IgE sera. Immunol Commun. 1977;6(5):473–482. doi: 10.3109/08820137709094146. [DOI] [PubMed] [Google Scholar]
  14. Malley A., Brandt C. J., Deppe L. B. Preparation and characterization of the anti-idiotypic properties of rabbit anti-timothy antigen B helper factor and anti-mouse timothy IgE antisera. Immunology. 1982 Feb;45(2):217–225. [PMC free article] [PubMed] [Google Scholar]
  15. Malley A., Harris R. L., Jr Biologic properties of a non-precipitating antigen from timothy pollen extracts. J Immunol. 1967 Oct;99(4):825–830. [PubMed] [Google Scholar]
  16. Moretta L., Mingari M. C., Romanzi C. A. Loss of Fc receptors for IgG from human T lymphocytes exposed to IgG immune complexes. Nature. 1978 Apr 13;272(5654):618–620. doi: 10.1038/272618a0. [DOI] [PubMed] [Google Scholar]
  17. Mota I., Wong D. Homologous and heterologous passive cutaneous anaphylactic activity of mouse antisera during the course of immunization. Life Sci. 1969 Aug 15;8(16):813–820. doi: 10.1016/0024-3205(69)90099-x. [DOI] [PubMed] [Google Scholar]
  18. Pierce S. K., Klinman N. R. Antibody-specific immunoregulation. J Exp Med. 1977 Aug 1;146(2):509–519. doi: 10.1084/jem.146.2.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rowley D. A., Fitch F. W., Stuart F. P., Köhler H., Cosenza H. Specific suppression of immune responses. Science. 1973 Sep 21;181(4105):1133–1141. doi: 10.1126/science.181.4105.1133. [DOI] [PubMed] [Google Scholar]
  20. Setcavage T. M., Kim Y. B. Inhibition of the immune response by Fc receptor-bound specific antibody on T lymphocytes. J Immunol. 1980 Feb;124(2):553–556. [PubMed] [Google Scholar]
  21. Sidman C. L., Unanue E. R. Control of B-lymphocyte function. I. Inactivation of mitogenesis by interactions with surface immunoglobulin and Fc-receptor molecules. J Exp Med. 1976 Oct 1;144(4):882–896. doi: 10.1084/jem.144.4.882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sy M. S., Bach B. A., Dohi Y., Nisonoff A., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. I. Induction of suppressor T cells with anti-idiotypic antibodies. J Exp Med. 1979 Nov 1;150(5):1216–1228. doi: 10.1084/jem.150.5.1216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Uhr J. W., Möller G. Regulatory effect of antibody on the immune response. Adv Immunol. 1968;8:81–127. doi: 10.1016/s0065-2776(08)60465-4. [DOI] [PubMed] [Google Scholar]
  24. Wysocki L. J., Sato V. L. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848. doi: 10.1073/pnas.75.6.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Zubler R. H., Benacerraf B., Germain R. N. Feedback suppression of the immune response in vitro. II. IgVH-restricted antibody-dependent suppression. J Exp Med. 1980 Mar 1;151(3):681–694. doi: 10.1084/jem.151.3.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Zubler R. H., Cantor H., Benacerraf B., Germain R. N. Feedback suppression of the immune response in vitro. I. Activity of antigen-stimulated B cells. J Exp Med. 1980 Mar 1;151(3):667–680. doi: 10.1084/jem.151.3.667. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES