Abstract
The enriched fractions of cytotoxic cells responsible for natural killer (NK) activity against both human sarcoma and neuroblastoma (LA-N2) cell lines were readily obtained by countercurrent centrifugal elutriation (CCE). The NK cells were obtained in the larger lymphocyte fractions (fraction 6 +/- 1), having a mean cell volume of 180 u3. The cytotoxic-enriched fraction contained 51% large lymphocytes having cytoplasmic granules. On the other hand, monocytes were purified to greater than 90% and isolated in another fraction (final fraction) and these cells had the lowest NK activity against both human tumour cell lines. However, compared with the lymphocyte fractions, small and large monocytes displayed greater antibody-dependent cellular cytotoxicity (ADCC) activity against human B erythrocytes. These results indicate that NK found to have activity against both tumour cells lines were larger lymphocytes, not small monocytes. Thus, countercurrent centrifugal elutriation (CCE) can provide a sensitive method to obtain enriched fractions of large lymphocytes contained tumoricidal activity against human sarcoma and neuroblastoma cell lines.
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- Froland S. S., Natvig J. B. Surface-bound immunoglobulin on lymphocytes from normal and immunodeficient humans. Scand J Immunol. 1972;1(1):1–12. doi: 10.1111/j.1365-3083.1972.tb03730.x. [DOI] [PubMed] [Google Scholar]
- Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kurnick J. T., Ostberg L., Stegagno M., Kimura A. K., Orn A., Sjöberg O. A rapid method for the separation of functional lymphoid cell populations of human and animal origin on PVP-silica (Percoll) density gradients. Scand J Immunol. 1979;10(6):563–573. doi: 10.1111/j.1365-3083.1979.tb01391.x. [DOI] [PubMed] [Google Scholar]
- Norris D. A., Morris R. M., Sanderson R. J., Kohler P. F. Isolation of functional subsets of human peripheral blood monocytes. J Immunol. 1979 Jul;123(1):166–172. [PubMed] [Google Scholar]
- Poplack D. G., Bonnard G. D., Holiman B. J., Blaese R. M. Monocyte-mediated antibody-dependent cellular cytotoxicity: a clinical test of monocyte function. Blood. 1976 Dec;48(6):809–816. [PubMed] [Google Scholar]
- Saksela E., Timonen T., Ranki A., Häyry P. Morphological and functional characterization of isolated effector cells responsible for human natural killer activity to fetal fibroblasts and to cultured cell line targets. Immunol Rev. 1979;44:71–123. doi: 10.1111/j.1600-065x.1979.tb00268.x. [DOI] [PubMed] [Google Scholar]
- Seeger R. C., Rayner S. A., Banerjee A., Chung H., Laug W. E., Neustein H. B., Benedict W. F. Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 1977 May;37(5):1364–1371. [PubMed] [Google Scholar]
- Timonen T., Ortaldo J. R., Herberman R. B. Characteristics of human large granular lymphocytes and relationship to natural killer and K cells. J Exp Med. 1981 Mar 1;153(3):569–582. doi: 10.1084/jem.153.3.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Timonen T., Saksela E. Isolation of human NK cells by density gradient centrifugation. J Immunol Methods. 1980;36(3-4):285–291. doi: 10.1016/0022-1759(80)90133-7. [DOI] [PubMed] [Google Scholar]
- Yasaka T., Mantich N. M., Boxer L. A., Baehner R. L. Functions of human monocyte and lymphocyte subsets obtained by countercurrent centrifugal elutriation: differing functional capacities of human monocyte subsets. J Immunol. 1981 Oct;127(4):1515–1518. [PubMed] [Google Scholar]