Skip to main content
Immunology logoLink to Immunology
. 1982 Apr;45(4):655–661.

Suppression of BCG cell wall-induced delayed-type hypersensitivity by BCG pre-treatment. II. Induction of suppressor T cells by heat-killed BCG injection.

K Kato, K Yamamoto
PMCID: PMC1555398  PMID: 6175566

Abstract

Previous intravenous (i.v.) injection of heat-killed Bacillus Calmette-Guérin (BCG) in mice produced a suppression of delayed-type hypersensitivity (DTH) induced with oil-treated BCG cell walls. This phenomenon was analysed by the macrophage migration inhibition (MI) test in which non-adherent spleen cells from mice which had been injected with heat-killed BCG (K-Non-ad. cells) were mixed with peritoneal exudate cells from BCG cell wall-immunized mice (effector-PEC). The test showed that the K-Non-ad. cells suppressed the MI activity in the effector-PEC, which indicated that the suppressor cells were induced in the spleen by the heat-killed BCG injections. Moreover, the suppressive effect of the K-Non-ad. cells disappeared after treatment with anti-brain associated theta serum (BA theta) and guinea-pig complement, and operated across the H-2 barrier. The suppressor cells inhibited the production or release of the migration inhibition factor from the BCG cell wall-sensitized cells in the presence of the specific antigen, purified protein derivatives (PPD). It was concluded that the injections of heat-killed BCG produced antigen non-specific suppressor T cells in DTH suppression.

Full text

PDF
655

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brehmer W., Anacker R. L., Ribi E. Immunogenicity of cell walls from various mycobacteria against airborne tuberculosis in mice. J Bacteriol. 1968 Jun;95(6):2000–2004. doi: 10.1128/jb.95.6.2000-2004.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coulis P. A., Lewert R. M., Fitch F. W. [Splenic suppressor cells and cell-mediated cytotoxicity in murine schistosomiasis]. J Immunol. 1978 Jan;120(1):58–60. [PubMed] [Google Scholar]
  3. Greene M. I., Sugimoto M., Benacerraf B. Mechanisms of regulation of cell-mediated immune responses. I. Effect of the route of immunization with TNP-coupled syngeneic cells on the induction and suppression of contact sensitivity to picryl chloride. J Immunol. 1978 May;120(5):1604–1611. [PubMed] [Google Scholar]
  4. Ha T. Y., Waksman B. H. Role of the thymus in tolerance. X. "Suppressor" activity of antigen-stimulated rat thymocytes transferred to normal recipients. J Immunol. 1973 May;110(5):1290–1299. [PubMed] [Google Scholar]
  5. Hanna N., Blanc S., Nelken D. Adjuvant-induced nonspecific supressor cells: in vitro and in vivo studies. Cell Immunol. 1980 Aug 1;53(2):225–235. doi: 10.1016/0008-8749(80)90324-x. [DOI] [PubMed] [Google Scholar]
  6. Kato K., Yamamoto K. I., Kakinuma M., Ishihara C., Azuma I. Suppression of BCG cell wall induced delayed-type hypersensitivity by BCG pre-treatment. I. Induction of adherent suppressor cells by live BCG injection and their characterization. Immunology. 1981 Feb;42(2):259–266. [PMC free article] [PubMed] [Google Scholar]
  7. Kirchner H., Holden H. T., Herberman Splenic suppressor macrophages induced in mice by injection of Corynebacterium parvum. J Immunol. 1975 Nov;115(5):1212–1216. [PubMed] [Google Scholar]
  8. Klimpel G. R., Henney C. S. BCG-induced suppressor cells. I. Demonstration of a macrophage-like suppressor cell that inhibits cytotoxic T cell generation in vitro. J Immunol. 1978 Feb;120(2):563–569. [PubMed] [Google Scholar]
  9. Kojima A., Egashira Y. Regulatory role of suppressor T cells in the expression of delayed-type hypersensitivity in mice. I. Transient appearance of suppressor T cells for the expression of delayed footpad reaction induced with lipid-conjugated lysozyme. Immunology. 1979 Jul;37(3):569–576. [PMC free article] [PubMed] [Google Scholar]
  10. Nakamura R. M., Tokunaga T. Induction of suppressor T cells in delayed-type hypersensitivity to Mycobacterium bovis BCG in low-responder mice. Infect Immun. 1980 May;28(2):331–335. doi: 10.1128/iai.28.2.331-335.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Orbach-Arbouys S., Castes M. Suppression of T-cell responses to histocompatibility antigens by BCG pre-treatment. Immunology. 1980 Feb;39(2):263–268. [PMC free article] [PubMed] [Google Scholar]
  12. Phanupak P., Moorhead J. W., Claman H. N. Tolerance and contact sensitivity to DNFB in mice. 3. Transfer of tolerance with "suppressor T cells". J Immunol. 1974 Oct;113(4):1230–1236. [PubMed] [Google Scholar]
  13. Polak L. Studies on the role of suppressor cells in specific unresponsiveness to DNCB. Immunology. 1976 Sep;31(3):425–432. [PMC free article] [PubMed] [Google Scholar]
  14. Ramos C., Schädtler-Siwon I., Ortiz-Ortiz L. Suppressor cells present in the spleens of Trypanosoma cruzi-infected mice. J Immunol. 1979 Apr;122(4):1243–1247. [PubMed] [Google Scholar]
  15. Scott M. T. Biological effects of the adjuvant Corynebacterium parvum. II. Evidence for macrophage-T-cell interaction. Cell Immunol. 1972 Nov;5(3):469–479. doi: 10.1016/0008-8749(72)90073-1. [DOI] [PubMed] [Google Scholar]
  16. Sy M. S., Miller S. D., Kowach H. B., Claman H. N. A splenic requirement for the generation of suppressor T cells. J Immunol. 1977 Dec;119(6):2095–2099. [PubMed] [Google Scholar]
  17. Tagart V. B., Thomas W. R., Asherson G. L. Suppressor T cells which block the induction of cytotoxic T cells in vivo. Immunology. 1978 Jun;34(6):1109–1116. [PMC free article] [PubMed] [Google Scholar]
  18. Turcotte R., Lafleur L., Labrèche M. Opposite effects of BCG on spleen and lymph node cells: lymphocyte proliferation and immunoglobulin synthesis. Infect Immun. 1978 Sep;21(3):696–704. doi: 10.1128/iai.21.3.696-704.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Watson S. R., Collins F. M. Development of suppressor T cells in mice heavily infected with mycobacteria. Immunology. 1980 Mar;39(3):367–373. [PMC free article] [PubMed] [Google Scholar]
  20. Zembala M., Asherson G. L. Depression of the T cell phenomenon of contact sensitivity by T cells from unresponsive mice. Nature. 1973 Jul 27;244(5413):227–228. doi: 10.1038/244227a0. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES