Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1982 Apr;45(4):769–774.

Isolation and functional characteristics of adherent phagocytic cells from mouse Peyer's patches.

T T MacDonald, P B Carter
PMCID: PMC1555428  PMID: 7068173

Abstract

Attempts were made to isolate adherent phagocytic cells (macrophages) from mouse Peyer's patch cell suspensions. Cell suspensions prepared by teasing apart the Peyer's patches contained no adherent phagocytic cells. However, if Peyer's patch fragments were treated with collagenase to disrupt the tissue matrix, cells prepared in this way contained a subpopulation of adherent phagocytic cells. These cells comprised only 0.1-0.2% of the total nucleated cell population of the Peyer's patch. Similar cells could also be isolated from the Peyer's patches of germ-free mice, but as judged by their ability to ingest opsonized erythrocytes, these cells were less activated than cells from the Peyer's patches of normal mice. Adherent cells from the Peyer's patches of normal mice could present antigen (ovalbumin) to T cells, and Peyer's patches cell suspensions containing adherent cells could be stimulated in vitro to produce an anti-sheep red blood cell plaque-forming cell response in the absence of 2-mercaptoethanol. These studies show that although the frequency of phagocytic adherent cells is extremely low in Peyer's patches, these cells have functions consistent with that of adherent cells in other lymphoid tissues.

Full text

PDF
769

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bienenstock J., Dolezel J. Peyer's patches: lack of specific antibody-containing cells after oral and parenteral immunization. J Immunol. 1971 Apr;106(4):938–945. [PubMed] [Google Scholar]
  2. Craig S. W., Cebra J. J. Peyer's patches: an enriched source of precursors for IgA-producing immunocytes in the rabbit. J Exp Med. 1971 Jul 1;134(1):188–200. doi: 10.1084/jem.134.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Faulk W. P., McCormick J. N., Goodman J. R., Yoffey J. M., Fudenberg H. H. Peyer's patches: morphologic studies. Cell Immunol. 1970 Nov;1(5):500–520. doi: 10.1016/0008-8749(70)90038-9. [DOI] [PubMed] [Google Scholar]
  4. Guy-Grand D., Griscelli C., Vassalli P. The gut-associated lymphoid system: nature and properties of the large dividing cells. Eur J Immunol. 1974 Jun;4(6):435–443. doi: 10.1002/eji.1830040610. [DOI] [PubMed] [Google Scholar]
  5. Guy-Grand D., Griscelli C., Vassalli P. The mouse gut T lymphocyte, a novel type of T cell. Nature, origin, and traffic in mice in normal and graft-versus-host conditions. J Exp Med. 1978 Dec 1;148(6):1661–1677. doi: 10.1084/jem.148.6.1661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HOWARD J. G., ROWLEY D., WARDLAW A. C. Investigations on the mechanism of stimulation of non-specific immunity by bacterial lipopolysaccharides. Immunology. 1958 Jul;1(3):181–203. [PMC free article] [PubMed] [Google Scholar]
  7. Hamburg S. I., Manejias R. E., Rabinovitch M. Macrophage activation: increased ingestion of IgG-coated erythrocytes after administration of interferon inducers to mice. J Exp Med. 1978 Feb 1;147(2):593–598. doi: 10.1084/jem.147.2.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kagnoff M. F., Campbell S. Functional characteristics of Peyer's patch lymphoid cells. I. Induction of humoral antibody and cell-mediated allograft reactions. J Exp Med. 1974 Feb 1;139(2):398–406. doi: 10.1084/jem.139.2.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kagnoff M. F. Functional characteristics of Peyer's patch cells. III. Carrier priming of T cells by antigen feeding. J Exp Med. 1975 Dec 1;142(6):1425–1435. doi: 10.1084/jem.142.6.1425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kiyono H., Babb J. L., Michalek S. M., McGhee J. R. Cellular basis for elevated IgA responses in C3H/HeJ mice. J Immunol. 1980 Aug;125(2):732–737. [PubMed] [Google Scholar]
  11. Krco C. J., Challacombe S. J., Lafuse W. P., David C. S., Tomasi T. B., Jr Expression of Ia antigens by mouse Peyer's patch cells. Cell Immunol. 1981 Jan 15;57(2):420–426. doi: 10.1016/0008-8749(81)90100-3. [DOI] [PubMed] [Google Scholar]
  12. LeFevre M. E., Hammer R., Joel D. D. Macrophages of the mammalian small intestine: a review. J Reticuloendothel Soc. 1979 Nov;26(5):553–573. [PubMed] [Google Scholar]
  13. MacDonald T. T., Carter P. B. Requirement for a bacterial flora before mice generate cells capable of mediating the delayed hypersensitivity reaction to sheep red blood cells. J Immunol. 1979 Jun;122(6):2624–2629. [PubMed] [Google Scholar]
  14. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Opitz H. G., Lemke H., Hewlett G. Activation of T-cells by a macrophage or 2-mercaptoethanol activated serum factor is essential for induction of a primary immune response to heterologous red cells in vitro. Immunol Rev. 1978;40:53–77. doi: 10.1111/j.1600-065x.1978.tb00401.x. [DOI] [PubMed] [Google Scholar]
  16. Owen R. L. Sequential uptake of horseradish peroxidase by lymphoid follicle epithelium of Peyer's patches in the normal unobstructed mouse intestine: an ultrastructural study. Gastroenterology. 1977 Mar;72(3):440–451. [PubMed] [Google Scholar]
  17. Pierce C. W. Immune responses in vitro. I. Cellular requirements for the immune response by nonprimed and primed spleen cells in vitro. J Exp Med. 1969 Aug 1;130(2):345–364. doi: 10.1084/jem.130.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Richman L. K., Graeff A. S., Strober W. Antigen presentation by macrophage-enriched cells from the mouse Peyer's patch. Cell Immunol. 1981 Jul 15;62(1):110–118. doi: 10.1016/0008-8749(81)90304-x. [DOI] [PubMed] [Google Scholar]
  19. Rosenstreich D. L., Mizel S. B. The participation of macrophages and macrophage cell lines in the activation of T lymphocytes by mitogens. Immunol Rev. 1978;40:102–135. doi: 10.1111/j.1600-065x.1978.tb00403.x. [DOI] [PubMed] [Google Scholar]
  20. Savage D. C., Dubos R., Schaedler R. W. The gastrointestinal epithelium and its autochthonous bacterial flora. J Exp Med. 1968 Jan 1;127(1):67–76. doi: 10.1084/jem.127.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schwartz R. H., Jackson L., Paul W. E. T lymphocyte-enriched murine peritoneal exudate cells. I. A reliable assay for antigen-induced T lymphocyte proliferation. J Immunol. 1975 Nov;115(5):1330–1338. [PubMed] [Google Scholar]
  22. Schwartz R. H., Yano A., Paul W. E. Interaction between antigen-presenting cells and primed T lymphocytes: an assessment of Ir gene expression in the antigen-presenting cell. Immunol Rev. 1978;40:153–180. doi: 10.1111/j.1600-065x.1978.tb00405.x. [DOI] [PubMed] [Google Scholar]
  23. Seeger R. C., Oppenheim J. J. Synergistic interaction of macrophages and lymphocytes in antigen-induced transformation of lymphocytes. J Exp Med. 1970 Jul 1;132(1):44–65. doi: 10.1084/jem.132.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Steinman R. M., Cohn Z. A. Identification of a novel cell type in peripheral lymphoid organs of mice. I. Morphology, quantitation, tissue distribution. J Exp Med. 1973 May 1;137(5):1142–1162. doi: 10.1084/jem.137.5.1142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Steinman R. M., Nussenzweig M. C. Dendritic cells: features and functions. Immunol Rev. 1980;53:127–147. doi: 10.1111/j.1600-065x.1980.tb01042.x. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES