Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1982 Oct;47(2):271–281.

Unusual cell surface properties of the T lymphocyte population expanding in MRL/Mp-lpr/lpr mice.

F Dumont, R G Habbersett
PMCID: PMC1555445  PMID: 6981590

Abstract

MRL/Mp-lpr/lpr (lpr/lpr) mice but not the congenic MRL/Mp-+/+ (+/+) mice, develop a generalized lymph node (LN) hypertrophy reflecting the expansion of a T-cell population that acts as an enhancing factor for autoimmunity. In order to characterize better this T-cell population, we investigated some of its surface properties in comparison with those of +/+ T cells. Electrophoretic measurements revealed that lpr/lpr T cells possess a lower electronegative surface charge than %/% T cells which indicates that the two cell types differ in the molecular composition of their plasmic membrane periphery. This notion was substantiated by the quantification of T- and B-cell markers and of lectin-binding sites on these cells using single- and two-colour flow cytofluorimetry. In agreement with recent observations by Lewis, Giorgi & Warner (1981) lpr/lpr T cells exhibited lower levels of Thy-1 and Lyt-1 antigens than +/+ T cells and were mostly devoid of Lyt-2 antigen. Although lpr/lpr lymph node (LN) cells displayed similar amounts of surface receptors for peanut agglutinin as +/+ LN cells, the expression of surface receptors for other lectins were either lower (Limulus polyphemus agglutinin, Maclura pomifera agglutinin, Concanavalin A) or higher (Helix pomatia agglutinin, Soya bean agglutinin, Bandeiraea simplicifolia agglutinin I, Phytohaemagglutinin L) on lpr/lpr T cells than on +/+ T cells. These data indicate that the T cells accumulating in hypertrophied lpr/lpr LN are endowed with unique surface characteristics which may explain some of the functional abnormalities of these cells.

Full text

PDF
271

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altman A., Theofilopoulos A. N., Weiner R., Katz D. H., Dixon F. J. Analysis of T cell function in autoimmune murine strains. Defects in production and responsiveness to interleukin 2. J Exp Med. 1981 Sep 1;154(3):791–808. doi: 10.1084/jem.154.3.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andrews B. S., Eisenberg R. A., Theofilopoulos A. N., Izui S., Wilson C. B., McConahey P. J., Murphy E. D., Roths J. B., Dixon F. J. Spontaneous murine lupus-like syndromes. Clinical and immunopathological manifestations in several strains. J Exp Med. 1978 Nov 1;148(5):1198–1215. doi: 10.1084/jem.148.5.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Creighton W. D., Katz D. H., Dixon F. J. Antigen-specific immunocompetency, B cell function, and regulatory helper and suppressor T cell activities in spontaneously autoimmune mice. J Immunol. 1979 Dec;123(6):2627–2636. [PubMed] [Google Scholar]
  4. Dumont F., Nardelli J. Peanut agglutinin (PNA)-binding properties of murine thymocyte subpopulation. Immunology. 1979 May;37(1):217–224. [PMC free article] [PubMed] [Google Scholar]
  5. Dumont F., Robert F. Changes in the electrophoretic mobility of mouse lymph node lymphocytes during ontogeny. Immunology. 1977 Jun;32(6):989–996. [PMC free article] [PubMed] [Google Scholar]
  6. Fowlkes B. J., Waxdal M. J., Sharrow S. O., Thomas C. A., 3rd, Asofsky R., Mathieson B. J. Differential binding of fluorescein-labeled lectins to mouse thymocytes: subsets revealed by flow microfluorometry. J Immunol. 1980 Aug;125(2):623–630. [PubMed] [Google Scholar]
  7. Irlé C., Piguet P. F., Vassalli P. In vitro maturation of immature thymocytes into immunocompetent T cells in the absence of direct thymic influence. J Exp Med. 1978 Jul 1;148(1):32–45. doi: 10.1084/jem.148.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones J. M., Feldman J. D. Binding of Maclura pomifera lectin to rat lymphoid cells and erythrocytes. J Immunol. 1973 Dec;111(6):1765–1770. [PubMed] [Google Scholar]
  9. Ledbetter J. A., Rouse R. V., Micklem H. S., Herzenberg L. A. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280–295. doi: 10.1084/jem.152.2.280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lewis D. E., Giorgi J. V., Warner N. L. Flow cytometry analysis of T cells and continuous T-cell lines from autoimmune MRL/l mice. Nature. 1981 Jan 22;289(5795):298–300. doi: 10.1038/289298a0. [DOI] [PubMed] [Google Scholar]
  11. Miller M. H., Powell J. I., Sharrow S. O., Schultz A. R. Rapid data collection, analysis, and graphics for flow microfluorometry instrumentation. Rev Sci Instrum. 1978 Aug;49(8):1137–1142. doi: 10.1063/1.1135535. [DOI] [PubMed] [Google Scholar]
  12. Murphy L. A., Goldstein I. J. Five alpha-D-galactopyranosyl-binding isolectins from Bandeiraea simplicifolia seeds. J Biol Chem. 1977 Jul 10;252(13):4739–4742. [PubMed] [Google Scholar]
  13. Pereira M. E., Kabat E. A. Immunochemical studies on lectins and their application to the fractionation of blood group substances and cells. CRC Crit Rev Immunol. 1979 Nov;1(1):33–78. [PubMed] [Google Scholar]
  14. Reisner Y., Linker-Israeli M., Sharon N. Separation of mouse thymocytes into two subpopulations by the use of peanut agglutinin. Cell Immunol. 1976 Jul;25(1):129–134. doi: 10.1016/0008-8749(76)90103-9. [DOI] [PubMed] [Google Scholar]
  15. Roche A. C., Schauer R., Monsigny M. Protein-sugar interactions. Purification by affinity chromatography of limulin, an N-acyl-neuraminidyl-binding protein. FEBS Lett. 1975 Oct 1;57(3):245–249. doi: 10.1016/0014-5793(75)80309-7. [DOI] [PubMed] [Google Scholar]
  16. Ruppert B. L., Ford R. J., Maizel A. L. T-cell mediated suppression in the MRL mouse. Cell Immunol. 1981 Jun;61(1):40–51. doi: 10.1016/0008-8749(81)90352-x. [DOI] [PubMed] [Google Scholar]
  17. Scollay R., Jacobs S., Jerabek L., Butcher E., Weissman I. T cell maturation: thymocyte and thymus migrant subpopulations defined with monoclonal antibodies to MHC region antigens. J Immunol. 1980 Jun;124(6):2845–2853. [PubMed] [Google Scholar]
  18. Steinberg A. D., Roths J. B., Murphy E. D., Steinberg R. T., Raveche E. S. Effects of thymectomy or androgen administration upon the autoimmune disease of MRL/Mp-lpr/lpr mice. J Immunol. 1980 Aug;125(2):871–873. [PubMed] [Google Scholar]
  19. Theofilopoulos A. N., Balderas R. S., Shawler D. L., Lee S., Dixon F. J. Influence of thymic genotype on the systemic lupus erythematosus-like disease and T cell proliferation of MRL/Mp-lpr/lpr mice. J Exp Med. 1981 Jun 1;153(6):1405–1414. doi: 10.1084/jem.153.6.1405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Theofilopoulos A. N., Dixon F. J. Etiopathogenesis of murine SLE. Immunol Rev. 1981;55:179–216. doi: 10.1111/j.1600-065x.1981.tb00343.x. [DOI] [PubMed] [Google Scholar]
  21. Theofilopoulos A. N., Eisenberg R. A., Bourdon M., Crowell J. S., Jr, Dixon F. J. Distribution of lymphocytes identified by surface markers in murine strains with systemic lupus erythematosus-like syndromes. J Exp Med. 1979 Feb 1;149(2):516–534. doi: 10.1084/jem.149.2.516. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES