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. 1982 Nov;47(3):459–468.

Immune responsiveness against the human placenta. I. Generation of cellular and humoral activity in experimental animals.

M Davies, M E McLaughlin, R G Sutcliffe
PMCID: PMC1555535  PMID: 7129527

Abstract

Using a new adaptation of the 51Cr release assay, it was found that plasma membrane vesicles from the human placental microvillous surface were not only capable of eliciting both cellular and humoral immunity in mice, but were also susceptible to lysis by these immune components. The production of cytotoxic cells and circulating antibody in immunized animals was only observed in the presence of Freund's complete adjuvant. The cells exhibited reactivity only against vesicles from a limited range of placentae, while the antibodies showed reactions against vesicles from a wider range of placentae. These vesicles were also susceptible to lysis by antisera raised against placental alkaline phosphatase but were resistant to attack by antibodies raised against normal human serum and HLA (multispecific) determinants and by a cytotoxic monoclonal antibody against human beta 2-microglobulin. The cellular cytotoxicity in the spleens of immunized animals could be abrogated if the cells were pretreated with AKR anti-C3H antiserum and complement. Further, in the spleens of in immunized animals, a population of cells was detected that exhibited a 'natural' cytotoxicity against several of the membrane preparations. This cytotoxicity was heat-labile, being abrogated if the cells were preincubated at 37 degrees for 4 hr before the assay, but was resistant to attack by the anti-Thy 1 antiserum. The results indicate that the use of 51Cr-labelled placental microvillous vesicles is a useful way of searching for anti-trophoblast immunity which may now be applied to the search for immune responses in pregnancy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Davies M., McLaughlin M. E., Sutcliffe R. G. Immune responsiveness against the human placenta. II. Response with xenogeneic lymphocytes in culture. Immunology. 1982 Nov;47(3):469–476. [PMC free article] [PubMed] [Google Scholar]
  2. Davies M., Parry J. E., Sutcliffe R. G. Examination of different preparations of human placental plasma membrane for the binding of insulin, transferrin and immunoglobulins. J Reprod Fertil. 1981 Nov;63(2):315–324. doi: 10.1530/jrf.0.0630315. [DOI] [PubMed] [Google Scholar]
  3. Davies M., Sabbadini E. Effect of BCG on alloimmune cell-mediated cytotoxicity in (C57BL/6Jfemale x A/Jmale)F1 mice. I. Effect of different BCG treatments on cytotoxicity mediated by T-cells and macrophages. J Natl Cancer Inst. 1978 May;60(5):1059–1068. doi: 10.1093/jnci/60.5.1059. [DOI] [PubMed] [Google Scholar]
  4. Davies M., Sutcliffe R. G. A quantitative assay for the measurement of immune responses directed against the human placenta. J Immunol Methods. 1982;48(3):373–383. doi: 10.1016/0022-1759(82)90338-6. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Smith N. C., Brush M. G., Luckett S. Preparation of human placental villous surface membrane. Nature. 1974 Nov 22;252(5481):302–303. doi: 10.1038/252302b0. [DOI] [PubMed] [Google Scholar]
  7. Stimson W. H., Strachan A. F., Shepherd A. Studies on the maternal immune response to placental antigens: absence of a blocking factor from the blood of abortion-prone women. Br J Obstet Gynaecol. 1979 Jan;86(1):41–45. doi: 10.1111/j.1471-0528.1979.tb10682.x. [DOI] [PubMed] [Google Scholar]
  8. Taylor P. V., Hancock K. W. Antigenicity of trophoblast and possible antigen-masking effects during pregnancy. Immunology. 1975 May;28(5):973–982. [PMC free article] [PubMed] [Google Scholar]
  9. Youtananukorn V., Mantangkasombut P. Specific plasma factors blocking human maternal cell-mediated immune reaction to placental antigens. Nat New Biol. 1973 Mar 28;242(117):110–111. doi: 10.1038/newbio242110a0. [DOI] [PubMed] [Google Scholar]
  10. Zinkernagel R. M., Doherty P. C. MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness. Adv Immunol. 1979;27:51–177. doi: 10.1016/s0065-2776(08)60262-x. [DOI] [PubMed] [Google Scholar]

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