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British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1997 Jul;121(5):849–851. doi: 10.1038/sj.bjp.0701270

Inhibition by heterologously-expressed P2Y2 nucleotide receptors of N-type calcium currents in rat sympathetic neurones

A K Filippov *, T E Webb *, E A Barnard *, D A Brown *,*
PMCID: PMC1564782  PMID: 9222539

Abstract

The P2Y2 nucleotide receptor has previously been shown to stimulate phosphoinositide breakdown. We now show that, when P2Y2 receptors are heterologously expressed by cRNA injection into dissociated rat sympathetic neurones, activation of these receptors by uridine 5′-triphosphate (UTP) or adenosine 5′-triphosphate (ATP) inhibits the N-type voltage-gated calcium current by ∼65%, with an IC50 of 0.5 μM. Thus, the same molecular species of nucleotide receptor can link to two different effector pathways.

Keywords: Nucleotide receptors, P2 receptors, uridine triphosphate, adenosine triphosphate, sympathetic neurones, calcium currents

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