Regulation of bradykinin B₂-receptor expression by oestrogen

Abstract

1. Tissue kallikrein is overexpressed in the kidney of female rats, this sexual dimorphism being associated with a greater effect of early blockade of bradykinin B₂-receptors on female blood pressure phenotype. We evaluated the effect of ovariectomy and oestradiol benzoate (50 μg kg⁻¹ every two days for two weeks) on the vasodepressor response to intra-arterial injection of bradykinin (150–900 ng kg⁻¹) and on the expression of bradykinin B₂-receptors.

2. Ovariectomy reduced the magnitude of the vasodepressor response to bradykinin and unmasked a secondary vasopressor effect. Oestrogen replacement restored the vasodepressor response to bradykinin in ovariectomized rats.

3. The vasodepressor responses to sodium nitroprusside (3–18 μg kg⁻¹), acetylcholine (30–600 ng kg⁻¹), desArg⁹-bradykinin (150–900 ng kg⁻¹) or prostaglandin E₂ (30–600 ng kg⁻¹) were significantly reduced by ovariectomy. Oestrogen restored to normal the responses to desArg⁹-bradykinin, acetylcholine and prostaglandin E₂, but not that to sodium nitroprusside.

4. B₂-receptor mRNA levels were decreased by ovariectomy in the aorta and kidney and they were restored to normal levels by oestrogen. Neither ovariectomy nor oestradiol affected receptor expression in the heart and uterus.

5. These results indicate that oestrogen regulates B₂-receptor gene expression and function. Since kinins exert a cardiovascular protective action, reduction in their vasodilator activity after menopause might contribute to the increased risk of pathological cardiovascular events. Conversely, the cardioprotective effects of oestrogen replacement might be, at least in part, mediated by activation of the kallikrein-kinin system.

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