Modulation of nicotinic ACh-, GABA_A- and 5-HT₃-receptor functions by external H-7, a protein kinase inhibitor, in rat sensory neurones

Abstract

1. The effects of external H-7, a potent protein kinase inhibitor, on the responses mediated by γ-aminobutyric acid A type (GABA_A)-, nicotinic acetylcholine (nicotinic ACh)-, ionotropic 5-hydroxytryptamine (5-HT₃)-, adenosine 5′-triphosphate (ATP)-, N-methyl-D-aspartate (NMDA)- and kainate (KA)-receptors were studied in freshly dissociated rat dorsal root ganglion neurone by use of whole cell patch-clamp technique.

2. External H-7 (1–1000 μM) produced a reversible, dose-dependent inhibition of whole cell currents activated by GABA, ACh and 5-HT.

3. Whole-cell currents evoked by ATP, 2-methylthio-ATP, NMDA and KA were insensitive to external H-7.

4. External H-7 shifted the dose-response curve of GABA-activated currents downward without changing the EC₅₀ significantly (from 15.0±4.0 μM to 18.0±5.0 μM). The maximum response to GABA was depressed by 34.0±5.3%. This inhibitory action of H-7 was voltage-independent.

5. Intracellular application of H-7 (20 μM), cyclic AMP (1 mM) and BAPTA (10 mM) could not reverse the H-7 inhibition of GABA-activated currents.

6. The results suggest that external H-7 selectively and allosterically modulates the functions of GABA_A-, nicotine ACh- and 5-HT₃ receptors via a common conserved site in the external domain of these receptors.

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