Distribution of P2Y receptor subtypes on haematopoietic cells

Abstract

1. RT–PCR-southern hybridization analyses with radiolabelled P2Y receptor cDNAs as probes indicated that the peripheral blood leukocytes and the human umbilical vein endothelial cells express P2Y₁, P2Y₂, P2Y₄ and P2Y₆ receptors.

2. Of the haematopoietic cell lines tested, promonocytic U937 cells express P2Y₂ and P2Y₆, but not P2Y₁ or P2Y₄; promyelocytic HL-60 cells express the P2Y₁, P2Y₂ and P2Y₆ receptors but not the P2Y₄ receptor; K562 cells express P2Y₁ but not P2Y₂, P2Y₄ or P2Y₆; and Dami cells express P2Y₁, P2Y₂, P2Y₄ and P2Y₆ receptors.

3. Of the peripheral blood leukocytes tested, polymorphonuclear cells express P2Y₄ and P2Y₆ but not P2Y₁ or P2Y₂ receptors; monocytes express P2Y₁, P2Y₂, P2Y₄ and P2Y₆ receptors and lymphocytes express P2Y₁, P2Y₂, P2Y₄ and P2Y₆ receptors.

4. These results suggest a physiological role for different P2Y receptor subtypes in the extracellular nucleotide-mediated stimulation of monocytes, neutrophils, lymphocytes and endothelial cells.

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