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British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1998 Jul;124(5):845–848. doi: 10.1038/sj.bjp.0701942

Culture substrate-specific expression of P2Y2 receptors in distal lung epithelial cells isolated from foetal rats

M T Clunes 1, A Collett 1, D L Baines 1, D L Bovell 2, H Murphie 1, S K Inglis 1, H L McAlroy 1, R E Olver 3, S M Wilson 1,*
PMCID: PMC1565477  PMID: 9692767

Abstract

ATP and UTP did not evoke [Ca2+]i signals in rat foetal lung epithelial cells grown on glass but elicited clear responses in cells grown into functionally polarised epithelia on permeable supports. Moreover, P2Y2 receptor mRNA could not be detected in cells on glass by the polymerase chain reaction but this mRNA species was clearly expressed by polarised cells. P2Y2 receptor expression thus appears to be a feature of the polarised phenotype.

Keywords: Epithelial polarity, polymerase chain reaction, apical membrane, nucleotide receptors, epithelial cells, lung, apical membrane

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