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. 1993 Mar;99:297–301. doi: 10.1289/ehp.9399297

Use of the glycophorin A human mutation assay to study workers exposed to styrene.

P J Compton-Quintana 1, R H Jensen 1, W L Bigbee 1, S G Grant 1, R G Langlois 1, M T Smith 1, S M Rappaport 1
PMCID: PMC1567018  PMID: 8319648

Abstract

The glycophorin A (GPA) assay is a human mutation assay that is potentially useful for large epidemiological studies. The assay is rapid and requires a minimal amount of blood, which can be stored before analysis. The data presented here were collected from workers exposed to styrene in a boat manufacturing plant. This study was the first to apply the GPA assay to an occupationally exposed population. Subjects with a mean styrene exposure of 30 ppm had a higher frequency of GPA N phi variant cells than subjects with mean exposure of 1 ppm, but the subjects differed in respect to smoking and age distribution. Results indicate that the original 1-W-1 version of the assay may not be suitable for studies of small numbers of exposed subjects due to variability and artifacts. The newer BR6 version, however, has much lower variability and shows promise for use in the occupational setting.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barale R. The genetic toxicology of styrene and styrene oxide. Mutat Res. 1991 Mar;257(2):107–126. doi: 10.1016/0165-1110(91)90021-m. [DOI] [PubMed] [Google Scholar]
  2. Bigbee W. L., Langlois R. G., Swift M., Jensen R. H. Evidence for an elevated frequency of in vivo somatic cell mutations in ataxia telangiectasia. Am J Hum Genet. 1989 Mar;44(3):402–408. [PMC free article] [PubMed] [Google Scholar]
  3. Bigbee W. L., Wyrobek A. J., Langlois R. G., Jensen R. H., Everson R. B. The effect of chemotherapy on the in vivo frequency of glycophorin A 'null' variant erythrocytes. Mutat Res. 1990 Mar;240(3):165–175. doi: 10.1016/0165-1218(90)90056-8. [DOI] [PubMed] [Google Scholar]
  4. Bodell W. J., Pongracz K., Kaur S., Burlingame A. L., Liu S. F., Rappaport S. M. Investigation of styrene oxide-DNA adducts and their detection in workers exposed to styrene. Prog Clin Biol Res. 1990;340C:271–282. [PubMed] [Google Scholar]
  5. Bond J. A. Review of the toxicology of styrene. Crit Rev Toxicol. 1989;19(3):227–249. doi: 10.3109/10408448909037472. [DOI] [PubMed] [Google Scholar]
  6. Compton P. J., Hooper K., Smith M. T. Human somatic mutation assays as biomarkers of carcinogenesis. Environ Health Perspect. 1991 Aug;94:135–141. doi: 10.1289/ehp.94-1567966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grant S. G., Campbell C. E., Duff C., Toth S. L., Worton R. G. Gene inactivation as a mechanism for the expression of recessive phenotypes. Am J Hum Genet. 1989 Oct;45(4):619–634. [PMC free article] [PubMed] [Google Scholar]
  8. Kyoizumi S., Nakamura N., Hakoda M., Awa A. A., Bean M. A., Jensen R. H., Akiyama M. Detection of somatic mutations at the glycophorin A locus in erythrocytes of atomic bomb survivors using a single beam flow sorter. Cancer Res. 1989 Feb 1;49(3):581–588. [PubMed] [Google Scholar]
  9. Langlois R. G., Bigbee W. L., Jensen R. H., German J. Evidence for increased in vivo mutation and somatic recombination in Bloom's syndrome. Proc Natl Acad Sci U S A. 1989 Jan;86(2):670–674. doi: 10.1073/pnas.86.2.670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Langlois R. G., Bigbee W. L., Jensen R. H., German J. Evidence for increased in vivo mutation and somatic recombination in Bloom's syndrome. Proc Natl Acad Sci U S A. 1989 Jan;86(2):670–674. doi: 10.1073/pnas.86.2.670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Langlois R. G., Bigbee W. L., Jensen R. H. Measurements of the frequency of human erythrocytes with gene expression loss phenotypes at the glycophorin A locus. Hum Genet. 1986 Dec;74(4):353–362. doi: 10.1007/BF00280485. [DOI] [PubMed] [Google Scholar]
  12. Langlois R. G., Nisbet B. A., Bigbee W. L., Ridinger D. N., Jensen R. H. An improved flow cytometric assay for somatic mutations at the glycophorin A locus in humans. Cytometry. 1990;11(4):513–521. doi: 10.1002/cyto.990110410. [DOI] [PubMed] [Google Scholar]
  13. Mäki-Paakkanen J., Walles S., Osterman-Golkar S., Norppa H. Single-strand breaks, chromosome aberrations, sister-chromatid exchanges, and micronuclei in blood lymphocytes of workers exposed to styrene during the production of reinforced plastics. Environ Mol Mutagen. 1991;17(1):27–31. doi: 10.1002/em.2850170105. [DOI] [PubMed] [Google Scholar]
  14. Straume T., Langlois R. G., Lucas J., Jensen R. H., Bigbee W. L., Ramalho A. T., Brandão-Mello C. E. Novel biodosimetry methods applied to victims of the Goiânia accident. Health Phys. 1991 Jan;60(1):71–76. doi: 10.1097/00004032-199101000-00011. [DOI] [PubMed] [Google Scholar]
  15. Yager J. W., Paradisin W. M., Symanski E., Rappaport S. M. Sister chromatid exchanges induced in peripheral lymphocytes of workers exposed to low concentrations of styrene. Prog Clin Biol Res. 1990;340C:347–356. [PubMed] [Google Scholar]

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