Abstract
32P-Postlabeling analysis and enzyme-linked immunosorbent assay (ELISA) have been used to detect DNA adducts in peripheral blood lymphocytes from primary aluminum production plant workers who were exposed occupationally to a mixture of polycyclic aromatic hydrocarbons (PAHs). Preliminary results reported here are from a comparative study being performed in two aluminum plants. The levels of aromatic DNA adducts have been determined by the 32P-postlabeling assay in samples collected on two occasions, 1 year apart. PAH-DNA adduct levels have also been determined by competitive ELISA in the second set of DNA samples. The results show the necessity of follow-up biomonitoring studies to detect possible alterations in biological effect induced by changing exposures. The comparison of the results obtained by 32P-postlabeling and ELISA may lead to a better understanding of the power and weaknesses of the two methods applied in these studies.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Perera F. P. Molecular cancer epidemiology: a new tool in cancer prevention. J Natl Cancer Inst. 1987 May;78(5):887–898. [PubMed] [Google Scholar]
- Poirier M. C., Santella R., Weinstein I. B., Grunberger D., Yuspa S. H. Quantitation of benzo(a)pyrene-deoxyguanosine adducts by radioimmunoassay. Cancer Res. 1980 Feb;40(2):412–416. [PubMed] [Google Scholar]
- Reddy M. V., Randerath K. Nuclease P1-mediated enhancement of sensitivity of 32P-postlabeling test for structurally diverse DNA adducts. Carcinogenesis. 1986 Sep;7(9):1543–1551. doi: 10.1093/carcin/7.9.1543. [DOI] [PubMed] [Google Scholar]
- Santella R. M., Weston A., Perera F. P., Trivers G. T., Harris C. C., Young T. L., Nguyen D., Lee B. M., Poirier M. C. Interlaboratory comparison of antisera and immunoassays for benzo[a]pyrene-diol-epoxide-I-modified DNA. Carcinogenesis. 1988 Jul;9(7):1265–1269. doi: 10.1093/carcin/9.7.1265. [DOI] [PubMed] [Google Scholar]
- Schoket B., Phillips D. H., Hewer A., Vincze I. 32P-postlabelling detection of aromatic DNA adducts in peripheral blood lymphocytes from aluminium production plant workers. Mutat Res. 1991 May;260(1):89–98. doi: 10.1016/0165-1218(91)90084-y. [DOI] [PubMed] [Google Scholar]
- Vahakangas K., Haugen A., Harris C. C. An applied synchronous fluorescence spectrophotometric assay to study benzo[a]pyrene-diolepoxide-DNA adducts. Carcinogenesis. 1985 Aug;6(8):1109–1115. doi: 10.1093/carcin/6.8.1109. [DOI] [PubMed] [Google Scholar]