Abstract
Exposure to environmental tobacco smoke (ETS), which is largely composed of the sidestream cigarette smoke, has been implicated in increased incidence of cancer among nonsmokers. The present study was conducted to compare the potential of mainstream and sidestream cigarette smoke to induce DNA adducts in mice. Groups of female C57Bl and DBA mice were exposed twice daily for 65-70 weeks to mainstream or sidestream smoke from the University of Kentucky reference cigarettes (2R1) in a nose-only exposure system. Animals received a total particulate matter dose of about 16 and 6 mg/kg body weight/exposure and exhibited blood carboxyhemoglobin levels of about 16 and 34%, for mainstream and sidestream smoke-exposed groups, respectively. Pulmonary aryl hydrocarbon hydroxylase (AHH) activity was induced by about 2- to 3-fold in both mainstream and sidestream groups of C57Bl and in mainstream smoke-exposed group of DBA mice, but not in sidestream smoke-exposed DBA mice. An analysis of total DNA adduct levels by the 32P-postlabeling assay showed a significant (12- to 25-fold) increase in the magnitude of preexisting lung DNA adducts in both mainstream and sidestream smoke-exposed C57Bl and DBA mice. Smoke exposures did not affect the total preexisting DNA adducts in liver of either strain. It is concluded that both mainstream and sidestream smoke are capable of enhancing preexisting DNA adducts in the lungs of chronically smoke-exposed mice.
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- Abramson R. K., Hutton J. J. Effects of cigarette smoking on aryl hydrocarbon hydroxylase activity in lungs and tissues of inbred mice. Cancer Res. 1975 Jan;35(1):23–29. [PubMed] [Google Scholar]
- Bond J. A., Chen B. T., Griffith W. C., Mauderly J. L. Inhaled cigarette smoke induces the formation of DNA adducts in lungs of rats. Toxicol Appl Pharmacol. 1989 Jun 1;99(1):161–172. doi: 10.1016/0041-008x(89)90121-x. [DOI] [PubMed] [Google Scholar]
- Gairola C. G. Free lung cell response of mice and rats to mainstream cigarette smoke exposure. Toxicol Appl Pharmacol. 1986 Jul;84(3):567–575. doi: 10.1016/0041-008x(86)90262-0. [DOI] [PubMed] [Google Scholar]
- Gairola C. G., Gupta R. C. Cigarette smoke-induced DNA adducts in the respiratory and nonrespiratory tissues of rats. Environ Mol Mutagen. 1991;17(4):253–257. doi: 10.1002/em.2850170406. [DOI] [PubMed] [Google Scholar]
- Gairola C. G. Pulmonary aryl hydrocarbon hydroxylase activity of mice, rats and guinea pigs following long term exposure to mainstream and sidestream cigarette smoke. Toxicology. 1987 Aug;45(2):177–184. doi: 10.1016/0300-483x(87)90103-x. [DOI] [PubMed] [Google Scholar]
- Gupta R. C., Sopori M. L., Gairola C. G. Formation of cigarette smoke-induced DNA adducts in the rat lung and nasal mucosa. Cancer Res. 1989 Apr 15;49(8):1916–1920. [PubMed] [Google Scholar]
- Phillips D. H., Hewer A., Martin C. N., Garner R. C., King M. M. Correlation of DNA adduct levels in human lung with cigarette smoking. Nature. 1988 Dec 22;336(6201):790–792. doi: 10.1038/336790a0. [DOI] [PubMed] [Google Scholar]
- Talaska G., al-Juburi A. Z., Kadlubar F. F. Smoking related carcinogen-DNA adducts in biopsy samples of human urinary bladder: identification of N-(deoxyguanosin-8-yl)-4-aminobiphenyl as a major adduct. Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5350–5354. doi: 10.1073/pnas.88.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weinstein I. B. Cigarette smoking and its fingerprint on DNA. J Natl Cancer Inst. 1988 Jun 15;80(8):548–549. doi: 10.1093/jnci/80.8.548. [DOI] [PubMed] [Google Scholar]