Abstract
The biopersistence of fibers and minerals in the respiratory tract is an important parameter in the toxicity of those materials. The biopersistence of respirable synthetic fibers and minerals in man can be most closely evaluated in an animal model. While acellular and in vitro systems are important for initial evaluation of solubility and durability, they cannot simulate the dynamics of inhalation deposition and clearance and the subsequent systemic reaction to fibers and minerals that occurs in the animal. To evaluate the biopersistence of synthetic fibers, male rats were exposed to a well defined rat respirable aerosol of man-made vitreous fibers (MMVF), 6 hr/day for 5 days. Following exposure, subgroups were sacrificed at intervals ranging from 1 hr to 52 weeks. Following sacrifice, the lungs were removed, weighed, and immediately frozen at 20 degrees C for subsequent digestion by low temperature plasma ashing. The number, size distribution, and chemical composition of the fibers in the aerosol and lung were determined. With this animal model the role of biopersistence in altering the geometry and clearance of fibers can be systematically evaluated. The model also can be applied for the evaluation of the biopersistence of nonfibrous minerals.
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Selected References
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- Bernstein D. M., Drew R. T., Kuschner M. Experimental approaches for exposure to sized glass fibers. Environ Health Perspect. 1980 Feb;34:47–57. doi: 10.1289/ehp.803447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cannon W. C., Blanton E. F., McDonald K. E. The flow-past chamber: an improved nose-only exposure system for rodents. Am Ind Hyg Assoc J. 1983 Dec;44(12):923–928. doi: 10.1080/15298668391405959. [DOI] [PubMed] [Google Scholar]
- Hesterberg T. W., Miiller W. C., Mast R., McConnell E. E., Bernstein D. M., Anderson R. Relationship between lung biopersistence and biological effects of man-made vitreous fibers after chronic inhalation in rats. Environ Health Perspect. 1994 Oct;102 (Suppl 5):133–137. doi: 10.1289/ehp.94102s5133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgan A., Holmes A. Solubility of rockwool fibres in vivo and the formation of pseudo-asbestos bodies. Ann Occup Hyg. 1984;28(3):307–314. doi: 10.1093/annhyg/28.3.307. [DOI] [PubMed] [Google Scholar]
- Musselman R. P., Miiller W. C., Eastes W., Hadley J. G., Kamstrup O., Thevenaz P., Hesterberg T. W. Biopersistences of man-made vitreous fibers and crocidolite fibers in rat lungs following short-term exposures. Environ Health Perspect. 1994 Oct;102 (Suppl 5):139–143. doi: 10.1289/ehp.94102s5139. [DOI] [PMC free article] [PubMed] [Google Scholar]