Abstract
A rapid, simple, and sensitive 125I-postlabeling technique has been developed to allow detection of DNA-protein cross-links induced by environmental contaminants and carcinogens. This method is based on specific incorporation of 125I into tyrosine residues associated with DNA. Cultured Chinese hamster ovary cells were exposed to various crosslinking agents, e.g., UV light, K2CrO4, or NiCl2. DNA was isolated by proteinase K/phenol/chloroform. The residual peptides cross-linked to DNA were radioiodinated with Na125I and chloramine T. After repeated precipitation with ethanol, the radioactivity was determined. The 125I method was compared with a 3[H]-tyrosine prelabeling method and found to be of similar sensitivity.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Coogan T. P., Latta D. M., Snow E. T., Costa M. Toxicity and carcinogenicity of nickel compounds. Crit Rev Toxicol. 1989;19(4):341–384. doi: 10.3109/10408448909029327. [DOI] [PubMed] [Google Scholar]
- Costa M. Analysis of DNA-protein complexes induced by chemical carcinogens. J Cell Biochem. 1990 Nov;44(3):127–135. doi: 10.1002/jcb.240440302. [DOI] [PubMed] [Google Scholar]
- Klein C. B., Frenkel K., Costa M. The role of oxidative processes in metal carcinogenesis. Chem Res Toxicol. 1991 Nov-Dec;4(6):592–604. doi: 10.1021/tx00024a001. [DOI] [PubMed] [Google Scholar]
- Lin X., Zhuang Z., Costa M. Analysis of residual amino acid--DNA crosslinks induced in intact cells by nickel and chromium compounds. Carcinogenesis. 1992 Oct;13(10):1763–1768. doi: 10.1093/carcin/13.10.1763. [DOI] [PubMed] [Google Scholar]
- Miller C. A., 3rd, Costa M. Analysis of proteins cross-linked to DNA after treatment of cells with formaldehyde, chromate, and cis-diamminedichloroplatinum(II). Mol Toxicol. 1989 Winter;2(1):11–26. [PubMed] [Google Scholar]
- Nackerdien Z., Rao G., Cacciuttolo M. A., Gajewski E., Dizdaroglu M. Chemical nature of DNA-protein cross-links produced in mammalian chromatin by hydrogen peroxide in the presence of iron or copper ions. Biochemistry. 1991 May 21;30(20):4873–4879. doi: 10.1021/bi00234a006. [DOI] [PubMed] [Google Scholar]
- Neuer B., Plagens U., Werner D. Phosphodiester bonds between polypeptides and chromosomal DNA. J Mol Biol. 1983 Feb 25;164(2):213–235. doi: 10.1016/0022-2836(83)90076-1. [DOI] [PubMed] [Google Scholar]
- Oleinick N. L., Chiu S. M., Ramakrishnan N., Xue L. Y. The formation, identification, and significance of DNA-protein cross-links in mammalian cells. Br J Cancer Suppl. 1987 Jun;8:135–140. [PMC free article] [PubMed] [Google Scholar]