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. 1994 Sep;102(Suppl 3):117–118. doi: 10.1289/ehp.94102s3117

Nickel-induced alterations in human renal epithelial cells.

A Haugen 1, L Maehle 1, S Mollerup 1, E Rivedal 1, D Ryberg 1
PMCID: PMC1567418  PMID: 7843084

Abstract

Cellular progression to malignancy appears to require a number of distinct steps in which genetic damage in key regulatory genes accumulates. Immortalization, or escape from senescence, is considered to be one of the first phenotypic changes. Ni2+ treatment of normal human kidney epithelial (NHKE) cells in vitro resulted in immortalization of the cells IHKE cells). The combined action of Ni2+ and v-Ha-ras oncogene fully transformed the cells to tumorigenicity in athymic nude mice. Sequence analysis of DNA from IHKE cells revealed point mutation in the p53 gene at codon 238 with T-->C transition. These findings suggest that Ni-induced mutation in the p53 gene can be involved in the immortalization of the NHKE cells. The results also show that changes in the responses to EGF and TGF beta and in the expression of their receptors occur during malignant progression in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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