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. 1984 Jun;56:205–212. doi: 10.1289/ehp.8456205

Effects of soybean flour on the pancreas of rats.

E E McGuinness, R G Morgan, K G Wormsley
PMCID: PMC1568217  PMID: 6207016

Abstract

We have reviewed the growth-promoting and carcinogenic effects of feeding raw soya flour to rats. If the raw soya flour-containing diets are fed for more than a year, about 10% of the animals develop pancreatic cancer. In addition, feeding raw soya flour markedly potentiates the action of even subthreshold amounts of pancreatic carcinogens. The raw soya flour therefore acts as a potent promoter, as well as a weak carcinogen. In view of this promotion, the rat fed raw soya flour is a sensitive model for screening pancreatic carcinogens. It is not known whether the human pancreas responds to dietary trypsin inhibitors in a manner similar to the rat. However, in view of the use of soya-based products in human nutrition--especially in infant foods--we urge that the effect of all soya-based products intended for human use be tested on the rat pancreas in long-term feeding studies, combined with subthreshold doses of azaserine to highlight any promoting activity of the product. It seems probable that if a product exerts no effect on the rat pancreas, the human pancreas will also be spared from noxious effects.

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Selected References

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  1. Anderson S. A., Chinn H. I., Fisher K. D. History and current status of infant formulas. Am J Clin Nutr. 1982 Feb;35(2):381–397. doi: 10.1093/ajcn/35.2.381. [DOI] [PubMed] [Google Scholar]
  2. BOOTH A. N., ROBBINS D. J., RIBELIN W. E., DEEDS F., SMITH A. K., RACKIS J. J. PROLONGED PANCREATIC HYPERTROPHY AND REVERSIBILITY IN RATS FED RAW SOYBEAN MEAL. Proc Soc Exp Biol Med. 1964 Aug-Sep;116:1067–1069. doi: 10.3181/00379727-116-29453. [DOI] [PubMed] [Google Scholar]
  3. Baintner K. Trypsin-inhibitor and chymotrypsin-inhibitor studies with soybean extracts. J Agric Food Chem. 1981 Jan-Feb;29(1):201–203. doi: 10.1021/jf00103a055. [DOI] [PubMed] [Google Scholar]
  4. Barlas N., Jensen R. T., Gardner J. D. Cholecystokinin-induced restricted stimulation of pancreatic enzyme secretion. Am J Physiol. 1982 May;242(5):G464–G469. doi: 10.1152/ajpgi.1982.242.5.G464. [DOI] [PubMed] [Google Scholar]
  5. Brand S. J., Morgan R. G. Stimulation of pancreatic secretion and growth in the rat after feeding cholestyramine. Gastroenterology. 1982 Oct;83(4):851–859. [PubMed] [Google Scholar]
  6. Calvert G. D., Yeates R. A. Adsorption of bile salts by soya-bean flour, wheat bran, lucerne (Medicago sativa), sawdust and lignin; the effect of saponins and other plant constituents. Br J Nutr. 1982 Jan;47(1):45–52. doi: 10.1079/bjn19820007. [DOI] [PubMed] [Google Scholar]
  7. Churella H. R., Yao B. C., Thomson W. A. Soybean trypsin inhibitor activity of soy infant formulas and its nutritional significance for the rat. J Agric Food Chem. 1976 Mar-Apr;24(2):393–397. doi: 10.1021/jf60204a051. [DOI] [PubMed] [Google Scholar]
  8. Crass R. A., Morgan R. G. Rapid changes in pancreatic DNA, RNA and protein in the rat during pancreatic enlargement and involution. Int J Vitam Nutr Res. 1981;51(1):85–91. [PubMed] [Google Scholar]
  9. Crass R. A., Morgan R. G. The effect of long-term feeding of soya-bean flour diets on pancreatic growth in the rat. Br J Nutr. 1982 Jan;47(1):119–129. doi: 10.1079/bjn19820017. [DOI] [PubMed] [Google Scholar]
  10. FARBER E. Carcinoma of the liver in rats fed ethionine. AMA Arch Pathol. 1956 Dec;62(6):445–453. [PubMed] [Google Scholar]
  11. Flavin D. F. The effects of soybean trypsin inhibitors on the pancreas of animals and man: a review. Vet Hum Toxicol. 1982 Feb;24(1):25–28. [PubMed] [Google Scholar]
  12. Fölsch U. R., Winckler K., Wormsley K. G. Effect of a soybean diet on enzyme content and ultrastructure of the rat exocrine pancreas. Digestion. 1974;11(3-4):161–171. doi: 10.1159/000197580. [DOI] [PubMed] [Google Scholar]
  13. Fölsch U. R., Winckler K., Wormsley K. G. Influence of repeated administration of cholecystokinin and secretin on the pancreas of the rat. Scand J Gastroenterol. 1978;13(6):663–671. doi: 10.3109/00365527809181779. [DOI] [PubMed] [Google Scholar]
  14. Fölsch U. R., Wormsley K. G. The pancreatic secretion of enzymes in rats treated with soybean diet. Scand J Gastroenterol. 1974;9(7):679–683. [PubMed] [Google Scholar]
  15. Green G. M., Lyman R. L. Feedback regulation of pancreatic enzyme secretion as a mechanism for trypsin inhibitor-induced hypersecretion in rats. Proc Soc Exp Biol Med. 1972 May;140(1):6–12. doi: 10.3181/00379727-140-36384. [DOI] [PubMed] [Google Scholar]
  16. Green G. M., Olds B. A., Matthews G., Lyman R. L. Protein, as a regulator of pancreatic enzyme secretion in the rat. Proc Soc Exp Biol Med. 1973 Apr;142(4):1162–1167. doi: 10.3181/00379727-142-37199. [DOI] [PubMed] [Google Scholar]
  17. Ihse I., Lilja P., Lundquist I. Trypsin as a regulator of pancreatic secretion in the rat. Scand J Gastroenterol. 1979;14(7):873–880. doi: 10.3109/00365527909181419. [DOI] [PubMed] [Google Scholar]
  18. Johnson L. R. Effects of gastrointestinal hormones on pancreatic growth. Cancer. 1981 Mar 15;47(6 Suppl):1640–1645. doi: 10.1002/1097-0142(19810315)47:6+<1640::aid-cncr2820471430>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  19. Khayambashi H., Lyman R. L. Secretion of rat pancreas perfused with plasma from rats fed soybean trypsin inhibitor. Am J Physiol. 1969 Sep;217(3):646–651. doi: 10.1152/ajplegacy.1969.217.3.646. [DOI] [PubMed] [Google Scholar]
  20. Krogdahl A., Holm H. Inhibition of human and rat pancreatic proteinases by crude and purified soybean proteinase inhibitors. J Nutr. 1979 Apr;109(4):551–558. doi: 10.1093/jn/109.4.551. [DOI] [PubMed] [Google Scholar]
  21. Krogdahl A., Holm H. Soybean proteinase inhibitors and human proteolytic enzymes: selective inactivation of inhibitors by treatment with human gastric juice. J Nutr. 1981 Dec;111(12):2045–2051. doi: 10.1093/jn/111.12.2045. [DOI] [PubMed] [Google Scholar]
  22. Lebenthal E., Choi T. S., Lee P. C. The development of pancreatic function in premature infants after milk-based and soy-based formulas. Pediatr Res. 1981 Sep;15(9):1240–1244. doi: 10.1203/00006450-198109000-00003. [DOI] [PubMed] [Google Scholar]
  23. Levison D. A., Morgan R. G., Brimacombe J. S., Hopwood D., Coghill G., Wormsley K. G. Carcinogenic effects of Di(2-hydroxypropyl)nitrosamine (DHPN) in male Wistar rats: promotion of pancreatic cancer by a raw soya flour diet. Scand J Gastroenterol. 1979;14(2):217–224. doi: 10.3109/00365527909179873. [DOI] [PubMed] [Google Scholar]
  24. Liener I. Significance for humans of biologically active factors in soybeans and other food legumes. J Am Oil Chem Soc. 1979 Mar;56(3):121–129. doi: 10.1007/BF02671433. [DOI] [PubMed] [Google Scholar]
  25. Lilja H. S., Curphey T. J., Yager J. D., Jr, Longnecker D. S. Persistence of DNA damage in rat pancreas following administration of three carcinogens and/or mutagens. Chem Biol Interact. 1978 Sep;22(2-3):287–295. doi: 10.1016/0009-2797(78)90133-3. [DOI] [PubMed] [Google Scholar]
  26. Longnecker D. S. Experimental pancreatic carcinogenesis. Lab Invest. 1982 May;46(5):543–544. [PubMed] [Google Scholar]
  27. Longnecker D. S., Roebuck B. D., Yager J. D., Jr, Lilja H. S., Siegmund B. Pancreatic carcinoma in azaserine-treated rats: induction, classification and dietary modulation of incidence. Cancer. 1981 Mar 15;47(6 Suppl):1562–1572. doi: 10.1002/1097-0142(19810315)47:6+<1562::aid-cncr2820471419>3.0.co;2-z. [DOI] [PubMed] [Google Scholar]
  28. McGuinness E. E., Hopwood D., Wormsley K. G. Further studies of the effects of raw soya flour on the rat pancreas. Scand J Gastroenterol. 1982 Mar;17(2):273–277. doi: 10.3109/00365528209182052. [DOI] [PubMed] [Google Scholar]
  29. McGuinness E. E., Hopwood D., Wormsley K. G. Potentiation of pancreatic carcinogenesis in the rat by DL-ethionine-induced pancreatitis. Scand J Gastroenterol. 1983 Mar;18(2):189–192. doi: 10.3109/00365528309181582. [DOI] [PubMed] [Google Scholar]
  30. McGuinness E. E., Morgan R. G., Levison D. A., Hopwood D., Wormsley K. G. Interaction of azaserine and raw soya flour on the rat pancreas. Scand J Gastroenterol. 1981;16(1):49–56. [PubMed] [Google Scholar]
  31. Melmed R. N., El-Aaser A. A., Holt S. J. Hypertrophy and hyperplasia of the neonatal rat exocrine pancreas induced by orally administered soybean trypsin inhibitor. Biochim Biophys Acta. 1976 Feb 24;421(2):280–288. doi: 10.1016/0304-4165(76)90294-4. [DOI] [PubMed] [Google Scholar]
  32. Milman H. A., Ward J. M., Chu K. C. Pancreatic carcinogenesis and naturally occurring pancreatic neoplasms of rats and mice in the NCI carcinogenesis testing program. J Environ Pathol Toxicol. 1978 Jul-Aug;1(6):829–840. [PubMed] [Google Scholar]
  33. Mohr U., Reznik G., Pour P. Carcinogenic effects of diisopropanolinitrosamine in Sprague-Dawley rats. J Natl Cancer Inst. 1977 Feb;58(2):361–366. doi: 10.1093/jnci/58.2.361. [DOI] [PubMed] [Google Scholar]
  34. Morgan R. G., Levinson D. A., Hopwood D., Saunders J. H., Wormsley K. G. Potentiation of the action of azaserine on the rat pancreas by raw soya bean flour. Cancer Lett. 1977 Jul;3(1-2):87–90. doi: 10.1016/s0304-3835(77)94455-x. [DOI] [PubMed] [Google Scholar]
  35. Morgan R. G., Wormsley K. G. Progress report. Cancer of the pancreas. Gut. 1977 Jul;18(7):580–596. doi: 10.1136/gut.18.7.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Naim M., Gertler A., Birk Y. The effect of dietary raw and autoclaved soya-bean protein fractions on growth, pancreatic enlargement and pancreatic enzymes in rats. Br J Nutr. 1982 Mar;47(2):281–288. doi: 10.1079/bjn19820037. [DOI] [PubMed] [Google Scholar]
  37. Oates P. S., Morgan R. G. Pancreatic growth and cell turnover in the rat fed raw soya flour. Am J Pathol. 1982 Aug;108(2):217–224. [PMC free article] [PubMed] [Google Scholar]
  38. Pugh T. D., King J. H., Koen H., Nychka D., Chover J., Wahba G., He Y. H., Goldfarb S. Reliable stereological method for estimating the number of microscopic hepatocellular foci from their transections. Cancer Res. 1983 Mar;43(3):1261–1268. [PubMed] [Google Scholar]
  39. Rackis J. J. Physiological properties of soybean trypsin inhibitors and their relationship to pancreatic hypertrophy and growth inhibition of rats. Fed Proc. 1965 Nov-Dec;24(6):1488–1493. [PubMed] [Google Scholar]
  40. Rackis J. J. Soybean protein: uses, problems, and potential. J Am Oil Chem Soc. 1977 Apr;54(4):290A–294A. doi: 10.1007/BF02670805. [DOI] [PubMed] [Google Scholar]
  41. Rall D. P. The role of laboratory animal studies in estimating carcinogenic risks for man. IARC Sci Publ. 1979;(25):179–189. [PubMed] [Google Scholar]
  42. Roebuck B. D., Yager J. D., Jr, Longnecker D. S. Dietary modulation of azaserine-induced pancreatic carcinogenesis in the rat. Cancer Res. 1981 Mar;41(3):888–893. [PubMed] [Google Scholar]
  43. Roebuck B. D., Yager J. D., Jr, Longnecker D. S., Wilpone S. A. Promotion by unsaturated fat of azaserine-induced pancreatic carcinogenesis in the rat. Cancer Res. 1981 Oct;41(10):3961–3966. [PubMed] [Google Scholar]
  44. Ryser H. J. Chemical carcinogenesis. N Engl J Med. 1971 Sep 23;285(13):721–734. doi: 10.1056/NEJM197109232851305. [DOI] [PubMed] [Google Scholar]
  45. Saffiotti U. Identification and definition of chemical carcinogens: review of criteria and research needs. J Toxicol Environ Health. 1980 Sep-Nov;6(5-6):1029–1057. doi: 10.1080/15287398009529925. [DOI] [PubMed] [Google Scholar]
  46. Silverman J., Adams J. D. N-nitrosamines in laboratory animal feed and bedding. Lab Anim Sci. 1983 Apr;33(2):161–164. [PubMed] [Google Scholar]
  47. Thomson W. A. Infant formulas and the use of vegetable protein. J Am Oil Chem Soc. 1979 Mar;56(3):386–388. doi: 10.1007/BF02671511. [DOI] [PubMed] [Google Scholar]
  48. Tomatis L. The predictive value of rodent carcinogenicity tests in the evaluation of human risks. Annu Rev Pharmacol Toxicol. 1979;19:511–530. doi: 10.1146/annurev.pa.19.040179.002455. [DOI] [PubMed] [Google Scholar]
  49. WEIBEL E. R. Principles and methods for the morphometric study of the lung and other organs. Lab Invest. 1963 Feb;12:131–155. [PubMed] [Google Scholar]
  50. Wolf W. J. Physical and chemical properties of soybean proteins. J Am Oil Chem Soc. 1977 Feb;54(2):112A–117A. doi: 10.1007/BF02912385. [DOI] [PubMed] [Google Scholar]
  51. Yanatori Y., Fujita T. Hypertrophy and hyperplasia in the endocrine and exocrine pancreas of rats fed soybean trypsin inhibitor or repeatedly injected with pancreozymin. Arch Histol Jpn. 1976 Mar;39(1):67–78. doi: 10.1679/aohc1950.39.67. [DOI] [PubMed] [Google Scholar]
  52. Young V. R., Scrimshaw N. S., Torun B., Viteri F. Soybean protein in human nutrition: an overview. J Am Oil Chem Soc. 1979 Mar;56(3):110–120. doi: 10.1007/BF02671432. [DOI] [PubMed] [Google Scholar]

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