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. 1980 Feb;34:103–111. doi: 10.1289/ehp.8034103

Putative inhibitory effects of chrysotile, crocidolite, and amosite mineral fibers on the more complex surface membrane glycolipids and glycoproteins

Howard A I Newman, Yeheskel A Saat, Ronald W Hart
PMCID: PMC1568522  PMID: 7389680

Abstract

Syrian hamster embryo cells were treated with galactose oxidase, followed by reduction with tritiated sodium borohydride at pH 7.4. The labeling patterns of galactosyl and N-acetyl galactosaminyl residues on the cell surface were altered in comparing scraped vs. unscraped and buffer vs. media-soaked cells treated with galactose oxidase. From these preliminary studies, the procedure to be used in most of the asbestos treatment studies was to treat cells in situ, in buffer with galactose oxidase, and then to label treated scraped cells with NaB3H4. After 20 hr interaction between chrysotile asbestos and Syrian hamster cell cultures, an alteration in surface labeling of glycolipids and glycoproteins was observed. Tritiated disialogangliosides (GDla) and the higher molecular weight labeled glycoproteins were significantly reduced by asbestos treatment.

Similar chrysotile asbestos-treated cultures were grown in monolayers in MEM (Eagles) with 10% fetal bovine serum for 2, 24, 48, and 72 hr and then surface-labeled with galactose oxidase–. NaB3H4 in phosphate buffer. Little or no difference was observed between surface-labeled lipid or protein distribution in untreated cells and those treated with asbestos for 2 hr. Asbestos-induced polar and neutral glycolipid pattern changes were observed at 24, 48, and 72 hr. Disialo- and trisialogangliosides (the more complex gangliosides) were decreased 85%, whereas globoside GL-4 was decreased by 60% at 72 hr. An overall decrease of labeled glycoproteins was observed at 24-48 hr. By 72 hr there was a complete loss of labeled protein bands with 80,000 dalton molecular mass. Since the changes in glycoproteins and glycolipids occur only after extended exposure of the cells to asbestos, the present studies support the concept that a metabolic rather than immediate masking effect is involved.

Comparisons of treatment of Syrian hamster embryo cells with various asbestos fibers for 48 hr in the order of decreasing reduction in complex gangliosides were crocidolite>chrysotile (intermediate)>amosite. Effects of the above fibers on high molecular weight glycoproteins labeling followed the same order. The labeling pattern is reminiscent of the increased simplification of glycolipids and glycoproteins found in transformed cells. In the case of asbestos which appears to have no independent mutagenic capability, it is more likely that the membrane changes induced by asbestos serve to allow other mutagens to pass into the cell so as to act on the nuclear structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brady R. O., Borek C., Bradley R. M. Composition and synthesis of gangliosides in rat hepatocyte and hepatoma cell lines. J Biol Chem. 1969 Dec 10;244(23):6552–6554. [PubMed] [Google Scholar]
  2. Chamberlain M., Tarmy E. M. Asbestos and glass fibres in bacterial mutation tests. Mutat Res. 1977 May;43(2):159–164. doi: 10.1016/0027-5107(77)90001-x. [DOI] [PubMed] [Google Scholar]
  3. Cheema P., Yogeeswaran G., Morris H. P., Murray R. K. Ganglioside patterns of three morris minimal deviation hepatomas. FEBS Lett. 1970 Dec;11(3):181–184. doi: 10.1016/0014-5793(70)80523-3. [DOI] [PubMed] [Google Scholar]
  4. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  5. Gahmberg C. G., Hakomori S. I. Altered growth behavior of malignant cells associated with changes in externally labeled glycoprotein and glycolipid. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3329–3333. doi: 10.1073/pnas.70.12.3329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gahmberg C. G., Hakomori S. Surface carbohydrates of hamster fibroblasts. II. Interaction of hamster NIL cell surfaces with Ricinus communis lectin and concanavalin A as revealed by surface galactosyl label. J Biol Chem. 1975 Apr 10;250(7):2447–2451. [PubMed] [Google Scholar]
  7. Hakomori S. Cell density-dependent changes of glycolipid concentrations in fibroblasts, and loss of this response in virus-transformed cells. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1741–1747. doi: 10.1073/pnas.67.4.1741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harington J. S., Allison A. C., Badami D. V. Mineral fibers: chemical, physicochemical, and biological properties. Adv Pharmacol Chemother. 1975;12(0):291–402. doi: 10.1016/s1054-3589(08)60223-9. [DOI] [PubMed] [Google Scholar]
  9. Laine R. A., Yoggeswaran G., Hakorori S. Glycosphingolipids covalently linked to agarose gel or glass beads. Use of the compounds for purification of antibodies directed against globoside and hematoside. J Biol Chem. 1974 Jul 25;249(14):4460–4466. [PubMed] [Google Scholar]
  10. Miller K., Kagan E. The in vivo effects of asbestos on macrophage membrane structure and population characteristics of macrophages: a scanning electron microscope study. J Reticuloendothel Soc. 1976 Aug;20(2):159–170. [PubMed] [Google Scholar]
  11. Sakiyama H., Gross S. K., Robbins P. W. Glycolipid synthesis in normal and virus-transformed hamster cell lines. Proc Natl Acad Sci U S A. 1972 Apr;69(4):872–876. doi: 10.1073/pnas.69.4.872. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  13. Yogeeswaran G., Laine R. A., Hakomori S. Mechanism of cell contact-dependent glycolipid synthesis: further studies with glycolipid-glass complex. Biochem Biophys Res Commun. 1974 Jul 24;59(2):591–599. doi: 10.1016/s0006-291x(74)80021-5. [DOI] [PubMed] [Google Scholar]
  14. Yogeeswaran G., Sheinin R., Wherrett J. R., Murray R. K. Studies on the glycosphingolipids of normal and virally transformed 3T3 mouse fibroblasts. J Biol Chem. 1972 Aug 25;247(16):5146–5148. [PubMed] [Google Scholar]

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