Abstract
During fine structure analyses of artificially induced waxy mutants in maize by use of Nelson's pollen analysis methods, it was noticed that some mutants showed a phenotype intermediate between waxy and normal nonwaxy. Such intermediate or leaky waxy mutants were frequent among mutants induced by a chemical mutagen, EMS. They seemed to be located evenly within the waxy locus. This would suggest that they might have been missense mutations, which would produce full-sized but partially inactivated enzymes, rather than deletions or frameshift mutations. To confirm the intermediate phenotype, a rapid measuring system was developed to measure waxiness of endosperm quantitatively. It was based on the blue value method and is applicable to a single grain of rice. Among the 27 waxy mutant lines of maize, including 11 EMS-induced, two of the EMS-induced mutants were clearly intermediate. Eighteen EMS induced wx mutants of rice were also examined, and nine were intermediate.
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- Amano E. Comparison of ethyl methanesulfonate- and radiation-induced waxy mutants in maize. Mutat Res. 1968 Jan-Feb;5(1):41–46. doi: 10.1016/0027-5107(68)90079-1. [DOI] [PubMed] [Google Scholar]
- Amano E., Smith H. H. Mutations induced by ethyl methanesulfonate in maize. Mutat Res. 1965 Aug;2(4):344–351. doi: 10.1016/0027-5107(65)90070-9. [DOI] [PubMed] [Google Scholar]
- Brookes P., Lawley P. D. The reaction of mono- and di-functional alkylating agents with nucleic acids. Biochem J. 1961 Sep;80(3):496–503. doi: 10.1042/bj0800496. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chourey P. S., Schwartz D. Ethyl methanesulfonate-induced mutations of the Sh1 protein in maize. Mutat Res. 1971 Jun;12(2):151–157. doi: 10.1016/0027-5107(71)90136-9. [DOI] [PubMed] [Google Scholar]
- Creech R G. Genetic Control of Carbohydrate Synthesis in Maize Endosperm. Genetics. 1965 Dec;52(6):1175–1186. doi: 10.1093/genetics/52.6.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kleinhofs A., Owais W. M., Nilan R. A. Azide. Mutat Res. 1978;55(3-4):165–195. doi: 10.1016/0165-1110(78)90003-9. [DOI] [PubMed] [Google Scholar]
- Loveless A. Possible relevance of O-6 alkylation of deoxyguanosine to the mutagenicity and carcinogenicity of nitrosamines and nitrosamides. Nature. 1969 Jul 12;223(5202):206–207. doi: 10.1038/223206a0. [DOI] [PubMed] [Google Scholar]
- Moore C. W., Creech R. G. Genetic Fine Structure Analysis of the AMYLOSE-EXTENDER Locus in ZEA MAYS L. Genetics. 1972 Apr;70(4):611–619. doi: 10.1093/genetics/70.4.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moore C. W., Creech R. G. Genetic Fine Structure Analysis of the AMYLOSE-EXTENDER Locus in ZEA MAYS L. Genetics. 1972 Apr;70(4):611–619. doi: 10.1093/genetics/70.4.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson O E. The Waxy Locus in Maize. I. Intralocus Recombination Frequency Estimates by Pollen and by Conventional Analyses. Genetics. 1962 Jun;47(6):737–742. doi: 10.1093/genetics/47.6.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson O. E. The Waxy Locus in Maize III. Effect of Structural Heterozygosity on Intragenic Recombination and Flanking Marker Assortment. Genetics. 1975 Jan;79(1):31–44. doi: 10.1093/genetics/79.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stadler L. J., Roman H. The Effect of X-Rays upon Mutation of the Gene A in Maize. Genetics. 1948 May;33(3):273–303. doi: 10.1093/genetics/33.3.273. [DOI] [PMC free article] [PubMed] [Google Scholar]