Abstract
Methods are being developed for derivatizing trace amounts of DNA adducts for ultimate determination by gas chromatographic techniques. High-pressure liquid chromatography is used to optimize appropriate derivatization reactions for the determination of cytosine. A single vial reaction scheme involves acylation with electrophoric pentafluorobenzoylchloride followed by alkylation with dimethyl sulfate. Currently, the overall yield for this reaction starting with 50 nmole of cytosine is 59 +/- 4.6%.
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Selected References
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- Mohamed G. B., Nazareth A., Hayes M. J., Giese R. W., Vouros P. Gas chromatography-mass spectrometry characteristics of methylated perfluoroacyl derivatives of cytosine and 5-methylcytosine. J Chromatogr. 1984 Nov 30;314:211–217. doi: 10.1016/s0021-9673(01)97735-0. [DOI] [PubMed] [Google Scholar]
- Nazareth A., Joppich M., Abdel-Baky S., O'Connell K., Sentissi A., Giese R. W. Electrophore-labeling and alkylation of standards of nucleic acid pyrimidine bases for analysis by gas chromatography with electron-capture detection. J Chromatogr. 1984 Nov 30;314:201–210. doi: 10.1016/s0021-9673(01)97734-9. [DOI] [PubMed] [Google Scholar]