Abstract
The mutagenic/carcinogenic activities of DEHP and MEHP were studied in bacteria and mammalian cells. MEHP but not DEHP exerted a dose-dependent DNA damaging effect to B. subtilis in Rec-assay. DEHP and MEHP showed mutagenic activities to S. typhimurium TA-100, with and without S-9 mix, respectively. MEHP produced not only the mutation in E. coli WP2B/r but also sister chromatid exchanges (SCE) in Chinese hamster V79 cells. It also induced 8AG/6TG-resistant gene mutations and chromosomal aberrations in the V79 cells. Transplacental administration of DEHP or MEHP to the Syrian golden hamster embryos was carried out by administering DEHP or MEHP to gravid animals on day 11 of gestation, followed by the cultivation of embryonic cells for 15-20 days. Both DEHP and MEHP induced 8AG/6TG-resistant mutation, chromosomal aberrations and morphological transformation in the embryonic cells of the Syrian golden hamster.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abe S., Sasaki M. Chromosome aberrations and sister chromatid exchanges in Chinese hamster cells exposed to various chemicals. J Natl Cancer Inst. 1977 Jun;58(6):1635–1641. doi: 10.1093/jnci/58.6.1635. [DOI] [PubMed] [Google Scholar]
- Inui N., Kaketomi M., Nishi Y. Mutagenic effects of AF-2, a food additive, on embryonic cells of the Syrian golden hamster on transplacental application. Mutat Res. 1976 Dec;41(2-3):351–360. doi: 10.1016/0027-5107(76)90108-1. [DOI] [PubMed] [Google Scholar]
- Ishidate M., Jr, Odashima S. Chromosome tests with 134 compounds on Chinese hamster cells in vitro--a screening for chemical carcinogens. Mutat Res. 1977 Jul;48(3-4):337–353. doi: 10.1016/0027-5107(77)90177-4. [DOI] [PubMed] [Google Scholar]
- Kada T., Tutikawa K., Sadaie Y. In vitro and host-mediated "rec-assay" procedures for screening chemical mutagens; and phloxine, a mutagenic red dye detected. Mutat Res. 1972 Oct;16(2):165–174. doi: 10.1016/0027-5107(72)90177-7. [DOI] [PubMed] [Google Scholar]
- Kuroki T., Drevon C., Montesano R. Microsome-mediated mutagenesis in V79 Chinese hamster cells by various nitrosamines. Cancer Res. 1977 Apr;37(4):1044–1050. [PubMed] [Google Scholar]
- Perry P., Evans H. J. Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange. Nature. 1975 Nov 13;258(5531):121–125. doi: 10.1038/258121a0. [DOI] [PubMed] [Google Scholar]
- Perry P., Wolff S. New Giemsa method for the differential staining of sister chromatids. Nature. 1974 Sep 13;251(5471):156–158. doi: 10.1038/251156a0. [DOI] [PubMed] [Google Scholar]
- Reznikoff C. A., Bertram J. S., Brankow D. W., Heidelberger C. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 1973 Dec;33(12):3239–3249. [PubMed] [Google Scholar]
- Singh A. R., Lawrence W. H., Autian J. Mutagenic and antifertility sensitivities of mice to di-2-ethylhexyl phthalate (DEHP) and dimethoxyethyl phthalate (DMEP). Toxicol Appl Pharmacol. 1974 Jul;29(1):35–46. doi: 10.1016/0041-008x(74)90159-8. [DOI] [PubMed] [Google Scholar]
- Stenchever M. A., Allen M. A., Jerominski L., Petersen R. V. Effects of bis(2-ethylhexyl) phthalate on chromosomes of human leukocytes and human fetal lung cells. J Pharm Sci. 1976 Nov;65(11):1648–1651. doi: 10.1002/jps.2600651121. [DOI] [PubMed] [Google Scholar]
- Yahagi T., Nagao M., Seino Y., Matsushima T., Sugimura T. Mutagenicities of N-nitrosamines on Salmonella. Mutat Res. 1977 Apr;48(2):121–129. doi: 10.1016/0027-5107(77)90151-8. [DOI] [PubMed] [Google Scholar]