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. 1997 Mar;9(3):335–353. doi: 10.1105/tpc.9.3.335

TOUSLED participates in apical tissue formation during gynoecium development in Arabidopsis.

J L Roe 1, J L Nemhauser 1, P C Zambryski 1
PMCID: PMC156922  PMID: 9090879

Abstract

Mutations at the TOUSLED (TSL) protein kinase locus in Arabidopsis cause reduced differentiation of apical gynoecial tissues and eliminate the fusion of the style and septum. TSL expression becomes confined to the developing style by stage 13, where it may promote expansion of tissues. Double mutant analysis suggests that ETTIN interacts with TSL, possibly by restricting TSL expression to apical regions. TSL, LEUNIG, and PERIANTHIA appear to participate in pathways of redundant function during the development of specific gynoecial tissues. TSL and LEUNIG most likely function in similar pathways during ovule development. TSL acts independently of the function of the organ identity genes AGAMOUS and APETALA2, and it is required for the formation of specific tissues in ectopic carpels. Mutations in TSL, ETTIN, PERIANTHIA, and LEUNIG all affect floral organ number as well as gynoecium morphology. Their respective wild-type loci must therefore play important roles in early floral meristem development during initiation of organ primordia in addition to their functions during regional differentiation within developing gynoecial primordia.

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Selected References

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  1. Becker D., Kemper E., Schell J., Masterson R. New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol Biol. 1992 Dec;20(6):1195–1197. doi: 10.1007/BF00028908. [DOI] [PubMed] [Google Scholar]
  2. Bowman J. L., Drews G. N., Meyerowitz E. M. Expression of the Arabidopsis floral homeotic gene AGAMOUS is restricted to specific cell types late in flower development. Plant Cell. 1991 Aug;3(8):749–758. doi: 10.1105/tpc.3.8.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bowman J. L., Smyth D. R., Meyerowitz E. M. Genes directing flower development in Arabidopsis. Plant Cell. 1989 Jan;1(1):37–52. doi: 10.1105/tpc.1.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clark S. E., Running M. P., Meyerowitz E. M. CLAVATA1, a regulator of meristem and flower development in Arabidopsis. Development. 1993 Oct;119(2):397–418. doi: 10.1242/dev.119.2.397. [DOI] [PubMed] [Google Scholar]
  5. Drews G. N., Bowman J. L., Meyerowitz E. M. Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product. Cell. 1991 Jun 14;65(6):991–1002. doi: 10.1016/0092-8674(91)90551-9. [DOI] [PubMed] [Google Scholar]
  6. Elliott R. C., Betzner A. S., Huttner E., Oakes M. P., Tucker W. Q., Gerentes D., Perez P., Smyth D. R. AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth. Plant Cell. 1996 Feb;8(2):155–168. doi: 10.1105/tpc.8.2.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gaiser J. C., Robinson-Beers K., Gasser C. S. The Arabidopsis SUPERMAN Gene Mediates Asymmetric Growth of the Outer Integument of Ovules. Plant Cell. 1995 Mar;7(3):333–345. doi: 10.1105/tpc.7.3.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gasser C. S., Robinson-Beers K. Pistil Development. Plant Cell. 1993 Oct;5(10):1231–1239. doi: 10.1105/tpc.5.10.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Honma M. A., Baker B. J., Waddell C. S. High-frequency germinal transposition of DsALS in Arabidopsis. Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6242–6246. doi: 10.1073/pnas.90.13.6242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jack T., Brockman L. L., Meyerowitz E. M. The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens. Cell. 1992 Feb 21;68(4):683–697. doi: 10.1016/0092-8674(92)90144-2. [DOI] [PubMed] [Google Scholar]
  11. Jofuku K. D., den Boer B. G., Van Montagu M., Okamuro J. K. Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. Plant Cell. 1994 Sep;6(9):1211–1225. doi: 10.1105/tpc.6.9.1211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Klucher K. M., Chow H., Reiser L., Fischer R. L. The AINTEGUMENTA gene of Arabidopsis required for ovule and female gametophyte development is related to the floral homeotic gene APETALA2. Plant Cell. 1996 Feb;8(2):137–153. doi: 10.1105/tpc.8.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kunst L., Klenz J. E., Martinez-Zapater J., Haughn G. W. AP2 Gene Determines the Identity of Perianth Organs in Flowers of Arabidopsis thaliana. Plant Cell. 1989 Dec;1(12):1195–1208. doi: 10.1105/tpc.1.12.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Liu Z., Meyerowitz E. M. LEUNIG regulates AGAMOUS expression in Arabidopsis flowers. Development. 1995 Apr;121(4):975–991. doi: 10.1242/dev.121.4.975. [DOI] [PubMed] [Google Scholar]
  15. Mizukami Y., Ma H. Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity. Cell. 1992 Oct 2;71(1):119–131. doi: 10.1016/0092-8674(92)90271-d. [DOI] [PubMed] [Google Scholar]
  16. Modrusan Z., Reiser L., Feldmann K. A., Fischer R. L., Haughn G. W. Homeotic Transformation of Ovules into Carpel-like Structures in Arabidopsis. Plant Cell. 1994 Mar;6(3):333–349. doi: 10.1105/tpc.6.3.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robinson-Beers K., Pruitt R. E., Gasser C. S. Ovule Development in Wild-Type Arabidopsis and Two Female-Sterile Mutants. Plant Cell. 1992 Oct;4(10):1237–1249. doi: 10.1105/tpc.4.10.1237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Roe J. L., Rivin C. J., Sessions R. A., Feldmann K. A., Zambryski P. C. The Tousled gene in A. thaliana encodes a protein kinase homolog that is required for leaf and flower development. Cell. 1993 Dec 3;75(5):939–950. doi: 10.1016/0092-8674(93)90537-z. [DOI] [PubMed] [Google Scholar]
  19. Running M. P., Meyerowitz E. M. Mutations in the PERIANTHIA gene of Arabidopsis specifically alter floral organ number and initiation pattern. Development. 1996 Apr;122(4):1261–1269. doi: 10.1242/dev.122.4.1261. [DOI] [PubMed] [Google Scholar]
  20. Sessions R. A., Zambryski P. C. Arabidopsis gynoecium structure in the wild and in ettin mutants. Development. 1995 May;121(5):1519–1532. doi: 10.1242/dev.121.5.1519. [DOI] [PubMed] [Google Scholar]
  21. Smyth D. R., Bowman J. L., Meyerowitz E. M. Early flower development in Arabidopsis. Plant Cell. 1990 Aug;2(8):755–767. doi: 10.1105/tpc.2.8.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Toriyama K., Thorsness M. K., Nasrallah J. B., Nasrallah M. E. A Brassica S locus gene promoter directs sporophytic expression in the anther tapetum of transgenic Arabidopsis. Dev Biol. 1991 Feb;143(2):427–431. doi: 10.1016/0012-1606(91)90094-j. [DOI] [PubMed] [Google Scholar]
  23. Weigel D., Meyerowitz E. M. The ABCs of floral homeotic genes. Cell. 1994 Jul 29;78(2):203–209. doi: 10.1016/0092-8674(94)90291-7. [DOI] [PubMed] [Google Scholar]

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