Abstract
The ultrastructural effects of mineral dusts on a macrophagelike cell line (P388D1) were studied by transmission electron microscopy. The cells were found to contain virus particles which probably did not exert a cytopathic effect toward the cells. During mitosis the asbestos fibers appeared to be pushed to the periphery of the cell. The distribution of the asbestos in the P388D1 cells was similar to that seen previously in cultured peritoneal macrophages, and it was concluded that the cytotoxic effect of asbestos towards the cells was due to mechanical effects, e.g., by penetrating structures such as the nucleus or phagosomes, by preventing the ordered movement of organelles with the cell or by disrupting the cytoskeletal framework of the cell.
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- ALLISON A. C., MALLUCCI L. HISTOCHEMICAL STUDIES OF LYSOSOMES AND LYSOSOMAL ENZYMES IN VIRUS-INFECTED CELL CULTURES. J Exp Med. 1965 Mar 1;121:463–476. doi: 10.1084/jem.121.3.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Allison A. C. Lysosomes and the toxicity of particulate pollutants. Arch Intern Med. 1971 Jul;128(1):131–139. [PubMed] [Google Scholar]
- Bernhard W. A new staining procedure for electron microscopical cytology. J Ultrastruct Res. 1969 May;27(3):250–265. doi: 10.1016/s0022-5320(69)80016-x. [DOI] [PubMed] [Google Scholar]
- Johnson N. F., Davies R. An ultrastructural study of the effects of asbestos fibres on cultured peritoneal macrophages. Br J Exp Pathol. 1981 Dec;62(6):559–570. [PMC free article] [PubMed] [Google Scholar]
- Koren H. S., Handwerger B. S., Wunderlich J. R. Identification of macrophage-like characteristics in a cultured murine tumor line. J Immunol. 1975 Feb;114(2 Pt 2):894–897. [PubMed] [Google Scholar]
- Morgan D. M., Vint S., Rideout J. M. Continuous flow fluorimetric assay of lysosomal enzymes. Med Lab Sci. 1978 Oct;35(4):335–341. [PubMed] [Google Scholar]
- Robock K. Standard quartz dq12 greater than 5 micro m for experimental pneumoconiosis research projects in the Federal Republic of Germany. Ann Occup Hyg. 1973 Apr;16(1):63–66. doi: 10.1093/annhyg/16.1.63. [DOI] [PubMed] [Google Scholar]
- Smith R. E., Fishman W. H. p-(acetoxymercuric) aniline diazotate, a reagent for visualizing the naphthol AS-BI product of acid hydrolase action at the level of the light and electron microscope. J Histochem Cytochem. 1969 Jan;17(1):1–22. doi: 10.1177/17.1.1. [DOI] [PubMed] [Google Scholar]