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. 1997 Apr;9(4):479–489. doi: 10.1105/tpc.9.4.479

The Promoter of the Gene Encoding the C4 Form of Phosphoenolpyruvate Carboxylase Directs Mesophyll-Specific Expression in Transgenic C4 Flaveria spp.

J Stockhaus 1, U Schlue 1, M Koczor 1, J A Chitty 1, W C Taylor 1, P Westhoff 1
PMCID: PMC156933  PMID: 12237362

Abstract

The function of the C4 mechanism of photosynthesis depends on the strict compartmentation of the enzymes involved. Here, we investigate the regulatory mechanisms that ensure the mesophyll-specific expression of the C4 isoform of phosphoenolpyruvate carboxylase. We show that 2 kb of the 5[prime] flanking region of the Flaveria trinervia C4 PpcA1 gene is sufficient to direct mesophyll-specific expression of the [beta]-glucuronidase reporter gene in transgenic F. bidentis (C4) plants. In young leaves of seedlings, the activity of this promoter is dependent on the developmental stage of the mesophyll cells. It is induced in a basipetal fashion (leaf tip to base) during leaf development. The promoter region of the orthologous nonphotosynthetic Ppc gene of F. pringlei (C3) induces reporter gene expression mainly in the vascular tissue of leaves and stems as well as in mesophyll cells of transgenic F. bidentis plants. Our experiments demonstrate that during the evolution of the C4 Flaveria species, cis-acting elements of the C4 Ppc gene must have been altered to achieve mesophyll-specific expression.

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Selected References

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  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  2. Hatch M. D. Regulation of enzymes in C4 photosynthesis. Curr Top Cell Regul. 1978;14:1–27. [PubMed] [Google Scholar]
  3. Hermans J., Westhoff P. Analysis of expression and evolutionary relationships of phosphoenolpyruvate carboxylase genes in Flaveria trinervia (C4) and F. pringlei (C3). Mol Gen Genet. 1990 Dec;224(3):459–468. doi: 10.1007/BF00262441. [DOI] [PubMed] [Google Scholar]
  4. Hermans J., Westhoff P. Homologous genes for the C4 isoform of phosphoenolpyruvate carboxylase in a C3 and a C4 Flaveria species. Mol Gen Genet. 1992 Aug;234(2):275–284. doi: 10.1007/BF00283848. [DOI] [PubMed] [Google Scholar]
  5. Jefferson R. A., Kavanagh T. A., Bevan M. W. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987 Dec 20;6(13):3901–3907. doi: 10.1002/j.1460-2075.1987.tb02730.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Langdale J. A., Nelson T. Spatial regulation of photosynthetic development in C4 plants. Trends Genet. 1991 Jun;7(6):191–196. doi: 10.1016/0168-9525(91)90435-s. [DOI] [PubMed] [Google Scholar]
  7. Langdale J. A., Zelitch I., Miller E., Nelson T. Cell position and light influence C4 versus C3 patterns of photosynthetic gene expression in maize. EMBO J. 1988 Dec 1;7(12):3643–3651. doi: 10.1002/j.1460-2075.1988.tb03245.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lazo G. R., Stein P. A., Ludwig R. A. A DNA transformation-competent Arabidopsis genomic library in Agrobacterium. Biotechnology (N Y) 1991 Oct;9(10):963–967. doi: 10.1038/nbt1091-963. [DOI] [PubMed] [Google Scholar]
  9. Matsuoka M., Kyozuka J., Shimamoto K., Kano-Murakami Y. The promoters of two carboxylases in a C4 plant (maize) direct cell-specific, light-regulated expression in a C3 plant (rice). Plant J. 1994 Sep;6(3):311–319. doi: 10.1046/j.1365-313x.1994.06030311.x. [DOI] [PubMed] [Google Scholar]
  10. Schäffner A. R., Sheen J. Maize C4 photosynthesis involves differential regulation of phosphoenolpyruvate carboxylase genes. Plant J. 1992 Mar;2(2):221–232. doi: 10.1046/j.1365-313x.1992.t01-44-00999.x. [DOI] [PubMed] [Google Scholar]
  11. Stockhaus J., Poetsch W., Steinmüller K., Westhoff P. Evolution of the C4 phosphoenolpyruvate carboxylase promoter of the C4 dicot Flaveria trinervia: an expression analysis in the C3 plant tobacco. Mol Gen Genet. 1994 Nov 1;245(3):286–293. doi: 10.1007/BF00290108. [DOI] [PubMed] [Google Scholar]
  12. Wang J. L., Turgeon R., Carr J. P., Berry J. O. Carbon Sink-to-Source Transition Is Coordinated with Establishment of Cell-Specific Gene Expression in a C4 Plant. Plant Cell. 1993 Mar;5(3):289–296. doi: 10.1105/tpc.5.3.289. [DOI] [PMC free article] [PubMed] [Google Scholar]

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