Skip to main content
NCBI home page
The Plant Cell logoLink to The Plant Cell
. 1997 May;9(5):731–743. doi: 10.1105/tpc.9.5.731

RED1 is necessary for phytochrome B-mediated red light-specific signal transduction in Arabidopsis.

D Wagner 1, U Hoecker 1, P H Quail 1
PMCID: PMC156952  PMID: 9165750

Abstract

Seedlings of a transgenic Arabidopsis line (ABO) that overexpresses phytochrome B (phyB) display enhanced deetiolation specifically in red light. To identify genetic loci necessary for phytochrome signal transduction in red light, we chemically mutagenized ABO seeds and screened M2 seedlings for revertants of the enhanced deetiolation response. One recessive, red light-specific extragenic revertant, designated red1, was isolated. The mutant phenotype was expressed in the original ABO background as well as in the nontransgenic Nossen (No-0) progenitor background. red1 is also deficient in several other aspects of red light-induced responses known to be mediated by phyB, such as inhibition of petiole elongation and the shade avoidance response. red1 was mapped to the bottom of chromosome 4 at a position distinct from all known photoreceptor loci. Together with complementation analysis, the data show that red1 is a novel photomorphogenic mutant. The evidence suggests that red1 represents a putative phytochrome signal transduction mutant potentially specific to the phyB pathway.

Full Text

The Full Text of this article is available as a PDF (2.3 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahmad M., Cashmore A. R. HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor. Nature. 1993 Nov 11;366(6451):162–166. doi: 10.1038/366162a0. [DOI] [PubMed] [Google Scholar]
  2. Ahmad M., Cashmore A. R. The pef mutants of Arabidopsis thaliana define lesions early in the phytochrome signaling pathway. Plant J. 1996 Dec;10(6):1103–1110. doi: 10.1046/j.1365-313x.1996.10061103.x. [DOI] [PubMed] [Google Scholar]
  3. Ang L. H., Deng X. W. Regulatory hierarchy of photomorphogenic loci: allele-specific and light-dependent interaction between the HY5 and COP1 loci. Plant Cell. 1994 May;6(5):613–628. doi: 10.1105/tpc.6.5.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barnes S. A., Quaggio R. B., Chua N. H. Phytochrome signal-transduction: characterization of pathways and isolation of mutants. Philos Trans R Soc Lond B Biol Sci. 1995 Oct 30;350(1331):67–74. doi: 10.1098/rstb.1995.0139. [DOI] [PubMed] [Google Scholar]
  5. Barnes W. M. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2216–2220. doi: 10.1073/pnas.91.6.2216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bell C. J., Ecker J. R. Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics. 1994 Jan 1;19(1):137–144. doi: 10.1006/geno.1994.1023. [DOI] [PubMed] [Google Scholar]
  7. Boylan M., Douglas N., Quail P. H. Dominant negative suppression of arabidopsis photoresponses by mutant phytochrome A sequences identifies spatially discrete regulatory domains in the photoreceptor. Plant Cell. 1994 Mar;6(3):449–460. doi: 10.1105/tpc.6.3.449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Castle L. A., Meinke D. W. A FUSCA gene of Arabidopsis encodes a novel protein essential for plant development. Plant Cell. 1994 Jan;6(1):25–41. doi: 10.1105/tpc.6.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cherry J. R., Hondred D., Walker J. M., Vierstra R. D. Phytochrome requires the 6-kDa N-terminal domain for full biological activity. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5039–5043. doi: 10.1073/pnas.89.11.5039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Chory J., Peto C., Feinbaum R., Pratt L., Ausubel F. Arabidopsis thaliana mutant that develops as a light-grown plant in the absence of light. Cell. 1989 Sep 8;58(5):991–999. doi: 10.1016/0092-8674(89)90950-1. [DOI] [PubMed] [Google Scholar]
  11. Chory J. Plant phototransduction. Phytochrome signal transduction. Curr Biol. 1994 Sep 1;4(9):844–846. doi: 10.1016/s0960-9822(00)00189-5. [DOI] [PubMed] [Google Scholar]
  12. Clack T., Mathews S., Sharrock R. A. The phytochrome apoprotein family in Arabidopsis is encoded by five genes: the sequences and expression of PHYD and PHYE. Plant Mol Biol. 1994 Jun;25(3):413–427. doi: 10.1007/BF00043870. [DOI] [PubMed] [Google Scholar]
  13. Dehesh K., Franci C., Parks B. M., Seeley K. A., Short T. W., Tepperman J. M., Quail P. H. Arabidopsis HY8 locus encodes phytochrome A. Plant Cell. 1993 Sep;5(9):1081–1088. doi: 10.1105/tpc.5.9.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Deng X. W., Caspar T., Quail P. H. cop1: a regulatory locus involved in light-controlled development and gene expression in Arabidopsis. Genes Dev. 1991 Jul;5(7):1172–1182. doi: 10.1101/gad.5.7.1172. [DOI] [PubMed] [Google Scholar]
  15. Deng X. W. Fresh view of light signal transduction in plants. Cell. 1994 Feb 11;76(3):423–426. doi: 10.1016/0092-8674(94)90107-4. [DOI] [PubMed] [Google Scholar]
  16. Deng X. W., Matsui M., Wei N., Wagner D., Chu A. M., Feldmann K. A., Quail P. H. COP1, an Arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a G beta homologous domain. Cell. 1992 Nov 27;71(5):791–801. doi: 10.1016/0092-8674(92)90555-q. [DOI] [PubMed] [Google Scholar]
  17. Edwards K., Johnstone C., Thompson C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res. 1991 Mar 25;19(6):1349–1349. doi: 10.1093/nar/19.6.1349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kim B. C., Soh M. C., Kang B. J., Furuya M., Nam H. G. Two dominant photomorphogenic mutations of Arabidopsis thaliana identified as suppressor mutations of hy2. Plant J. 1996 Apr;9(4):441–456. doi: 10.1046/j.1365-313x.1996.09040441.x. [DOI] [PubMed] [Google Scholar]
  19. Konieczny A., Ausubel F. M. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J. 1993 Aug;4(2):403–410. doi: 10.1046/j.1365-313x.1993.04020403.x. [DOI] [PubMed] [Google Scholar]
  20. Li J., Nagpal P., Vitart V., McMorris T. C., Chory J. A role for brassinosteroids in light-dependent development of Arabidopsis. Science. 1996 Apr 19;272(5260):398–401. doi: 10.1126/science.272.5260.398. [DOI] [PubMed] [Google Scholar]
  21. Millar A. J., McGrath R. B., Chua N. H. Phytochrome phototransduction pathways. Annu Rev Genet. 1994;28:325–349. doi: 10.1146/annurev.ge.28.120194.001545. [DOI] [PubMed] [Google Scholar]
  22. Nagatani A., Reed J. W., Chory J. Isolation and Initial Characterization of Arabidopsis Mutants That Are Deficient in Phytochrome A. Plant Physiol. 1993 May;102(1):269–277. doi: 10.1104/pp.102.1.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Parks B. M., Quail P. H. hy8, a new class of arabidopsis long hypocotyl mutants deficient in functional phytochrome A. Plant Cell. 1993 Jan;5(1):39–48. doi: 10.1105/tpc.5.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Pepper A., Delaney T., Washburn T., Poole D., Chory J. DET1, a negative regulator of light-mediated development and gene expression in arabidopsis, encodes a novel nuclear-localized protein. Cell. 1994 Jul 15;78(1):109–116. doi: 10.1016/0092-8674(94)90577-0. [DOI] [PubMed] [Google Scholar]
  25. Quail P. H., Boylan M. T., Parks B. M., Short T. W., Xu Y., Wagner D. Phytochromes: photosensory perception and signal transduction. Science. 1995 May 5;268(5211):675–680. doi: 10.1126/science.7732376. [DOI] [PubMed] [Google Scholar]
  26. Quail P. H., Briggs W. R., Chory J., Hangarter R. P., Harberd N. P., Kendrick R. E., Koornneef M., Parks B., Sharrock R. A., Schafer E. Spotlight on Phytochrome Nomenclature. Plant Cell. 1994 Apr;6(4):468–471. doi: 10.1105/tpc.6.4.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Quail P. H. Photosensory perception and signal transduction in plants. Curr Opin Genet Dev. 1994 Oct;4(5):652–661. doi: 10.1016/0959-437x(94)90131-l. [DOI] [PubMed] [Google Scholar]
  28. Reed J. W., Nagpal P., Poole D. S., Furuya M., Chory J. Mutations in the gene for the red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development. Plant Cell. 1993 Feb;5(2):147–157. doi: 10.1105/tpc.5.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Schmidt R., West J., Cnops G., Love K., Balestrazzi A., Dean C. Detailed description of four YAC contigs representing 17 Mb of chromosome 4 of Arabidopsis thaliana ecotype Columbia. Plant J. 1996 May;9(5):755–765. doi: 10.1046/j.1365-313x.1996.9050755.x. [DOI] [PubMed] [Google Scholar]
  30. Sharrock R. A., Quail P. H. Novel phytochrome sequences in Arabidopsis thaliana: structure, evolution, and differential expression of a plant regulatory photoreceptor family. Genes Dev. 1989 Nov;3(11):1745–1757. doi: 10.1101/gad.3.11.1745. [DOI] [PubMed] [Google Scholar]
  31. Somers D. E., Sharrock R. A., Tepperman J. M., Quail P. H. The hy3 Long Hypocotyl Mutant of Arabidopsis Is Deficient in Phytochrome B. Plant Cell. 1991 Dec;3(12):1263–1274. doi: 10.1105/tpc.3.12.1263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Szekeres M., Németh K., Koncz-Kálmán Z., Mathur J., Kauschmann A., Altmann T., Rédei G. P., Nagy F., Schell J., Koncz C. Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell. 1996 Apr 19;85(2):171–182. doi: 10.1016/s0092-8674(00)81094-6. [DOI] [PubMed] [Google Scholar]
  33. Takahashi T., Gasch A., Nishizawa N., Chua N. H. The DIMINUTO gene of Arabidopsis is involved in regulating cell elongation. Genes Dev. 1995 Jan 1;9(1):97–107. doi: 10.1101/gad.9.1.97. [DOI] [PubMed] [Google Scholar]
  34. Valvekens D., Van Montagu M., Van Lijsebettens M. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536–5540. doi: 10.1073/pnas.85.15.5536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Wagner D., Fairchild C. D., Kuhn R. M., Quail P. H. Chromophore-bearing NH2-terminal domains of phytochromes A and B determine their photosensory specificity and differential light lability. Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4011–4015. doi: 10.1073/pnas.93.9.4011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Wagner D., Koloszvari M., Quail P. H. Two Small Spatially Distinct Regions of Phytochrome B Are Required for Efficient Signaling Rates. Plant Cell. 1996 May;8(5):859–871. doi: 10.1105/tpc.8.5.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Wagner D., Quail P. H. Mutational analysis of phytochrome B identifies a small COOH-terminal-domain region critical for regulatory activity. Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8596–8600. doi: 10.1073/pnas.92.19.8596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Wagner D., Tepperman J. M., Quail P. H. Overexpression of Phytochrome B Induces a Short Hypocotyl Phenotype in Transgenic Arabidopsis. Plant Cell. 1991 Dec;3(12):1275–1288. doi: 10.1105/tpc.3.12.1275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Wei N., Chamovitz D. A., Deng X. W. Arabidopsis COP9 is a component of a novel signaling complex mediating light control of development. Cell. 1994 Jul 15;78(1):117–124. doi: 10.1016/0092-8674(94)90578-9. [DOI] [PubMed] [Google Scholar]
  40. Whitelam G. C., Johnson E., Peng J., Carol P., Anderson M. L., Cowl J. S., Harberd N. P. Phytochrome A null mutants of Arabidopsis display a wild-type phenotype in white light. Plant Cell. 1993 Jul;5(7):757–768. doi: 10.1105/tpc.5.7.757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Xu Y., Parks B. M., Short T. W., Quail P. H. Missense mutations define a restricted segment in the C-terminal domain of phytochrome A critical to its regulatory activity. Plant Cell. 1995 Sep;7(9):1433–1443. doi: 10.1105/tpc.7.9.1433. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Plant Cell are provided here courtesy of Oxford University Press

RESOURCES