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. 2006 Sep;174(1):29–39. doi: 10.1534/genetics.106.060152

TABLE 1.

Characteristics of genetic markers in this linkage map

Probe Marker type Source No. of probes No. of loci Status Reference
RFLP EST_BIF Immature flower library of B. rapa cv. Jangwon 114 157 pBluescript Kim et al. (1996); Lim et al. (2000)
EST_BAN Anther library of B. rapa cv. Jangwon 176 235 pBluescript
EST_BDS Dark-grown seeding library of B. rapa cv. Jangwon 45 55 pBluescript
EST_BR Root library of B. rapa cv. Jangwon 24 27 pBluescript
At4GTS Chromosome 4-located genes of Arabidopsis cv. Colombia 10 10 pGemT GenBank
At5GTS Chromosome 5-located genes of Arabidopsis cv. Colombia 14 17 pGemT GenBank
AtGene Full length of expressed gene from A. thaliana 2 2 pGemT GenBank
BrGene Full length of expressed gene from B.rapa 11 17 pGemT BrFLC_AY273164; BrAGL20_AY345237, etc.
PCR SSR R1–R10-located SSR markers 11 11 Primer Lowe et al. (2004)
SSR R1–R10-located SSR markers 10 10 Primer Suwabe et al. (2002)
BrH80A08_FLC1 5′-ttcccaagcttgctggtact-3′ 5′-gagatttccctcgcttgatg-3′ 1 1 Primer KBrH80A08_AC155344
BrH04D11_FLC2 5′-gcgccaattataaatttgattttc-3′ 5′-tcctcctgaacctggtcttg-3′ 1 1 Primer KBrH04D11_AC155341
BrH80C09_MAF 5′-cagtgaagttcaaccgcagta-3′ 5′-catgagtgaacataaaacagtgaaa-3′ 1 1 Primer KBrH80C09_A166741
Random primer Operon primer S14 1 1 Primer BM commercial product
Total 421 545