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. 1997 Aug;9(8):1425–1433. doi: 10.1105/tpc.9.8.1425

Specific Binding of the Syringolide Elicitors to a Soluble Protein Fraction from Soybean Leaves.

C Ji 1, Y Okinaka 1, Y Takeuchi 1, T Tsurushima 1, R I Buzzell 1, J J Sims 1, S L Midland 1, D Slaymaker 1, M Yoshikawa 1, N Yamaoka 1, N T Keen 1
PMCID: PMC157008  PMID: 12237390

Abstract

Syringolides are glycolipid elicitors produced by Gram-negative bacteria expressing Pseudomonas syringae avirulence gene D. The syringolides mediate gene-for-gene complementarity, inducing the hypersensitive response only in soybean plants carrying the Rpg4 disease resistance gene. A site(s) for 125I-syringolide 1 was detected in the soluble protein fraction from soybean leaves, but no evidence for ligand-specific binding to the microsomal fraction was obtained. The Kd value for syringolide 1 binding with the soluble fraction was 8.7 nM, and binding was greatly reduced by prior protease treatment or heating. A native gel assay was also used to demonstrate ligand-specific binding of labeled syringolide 1 with a soluble protein(s). Competition studies with 125I-syringolide 1 and several structural derivatives demonstrated a direct correlation between binding affinity to the soluble fraction and elicitor activity. However, differential competition binding studies disclosed no differences in syringolide binding to soluble fractions from Rpg4/Rpg4 or rpg4/rpg4 soybean leaves. Thus, the observed binding site fulfills several criteria expected of an intracellular receptor for the syringolides, but it is most likely not encoded by the Rpg4 gene. Instead, the Rpg4 gene product may function subsequent to elicitor binding, possibly in intracellular signal transduction.

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Selected References

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  1. Atkinson M. M., Midland S. L., Sims J. J., Keen N. T. Syringolide 1 Triggers Ca2+ Influx, K+ Efflux, and Extracellular Alkalization in Soybean Cells Carrying the Disease-Resistance Gene Rpg4. Plant Physiol. 1996 Sep;112(1):297–302. doi: 10.1104/pp.112.1.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bent A. F. Plant Disease Resistance Genes: Function Meets Structure. Plant Cell. 1996 Oct;8(10):1757–1771. doi: 10.1105/tpc.8.10.1757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bruns R. F., Lawson-Wendling K., Pugsley T. A. A rapid filtration assay for soluble receptors using polyethylenimine-treated filters. Anal Biochem. 1983 Jul 1;132(1):74–81. doi: 10.1016/0003-2697(83)90427-x. [DOI] [PubMed] [Google Scholar]
  4. Gopalan S., Bauer D. W., Alfano J. R., Loniello A. O., He S. Y., Collmer A. Expression of the Pseudomonas syringae avirulence protein AvrB in plant cells alleviates its dependence on the hypersensitive response and pathogenicity (Hrp) secretion system in eliciting genotype-specific hypersensitive cell death. Plant Cell. 1996 Jul;8(7):1095–1105. doi: 10.1105/tpc.8.7.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grant M. R., Godiard L., Straube E., Ashfield T., Lewald J., Sattler A., Innes R. W., Dangl J. L. Structure of the Arabidopsis RPM1 gene enabling dual specificity disease resistance. Science. 1995 Aug 11;269(5225):843–846. doi: 10.1126/science.7638602. [DOI] [PubMed] [Google Scholar]
  6. Hahn M. G. Microbial elicitors and their receptors in plants. Annu Rev Phytopathol. 1996;34:387–412. doi: 10.1146/annurev.phyto.34.1.387. [DOI] [PubMed] [Google Scholar]
  7. Keen N. T. Gene-for-gene complementarity in plant-pathogen interactions. Annu Rev Genet. 1990;24:447–463. doi: 10.1146/annurev.ge.24.120190.002311. [DOI] [PubMed] [Google Scholar]
  8. Kooman-Gersmann M., Honee G., Bonnema G., De Wit PJGM. A High-Affinity Binding Site for the AVR9 Peptide Elicitor of Cladosporium fulvum Is Present on Plasma Membranes of Tomato and Other Solanaceous Plants. Plant Cell. 1996 May;8(5):929–938. doi: 10.1105/tpc.8.5.929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leach J. E., White F. F. Bacterial avirulence genes. Annu Rev Phytopathol. 1996;34:153–179. doi: 10.1146/annurev.phyto.34.1.153. [DOI] [PubMed] [Google Scholar]
  10. Méster J., Rbertson D. M., Feherty P., Kellie A. E. Determination of high-affinity oestrogen receptor sites in uterine supernatant preparations. Biochem J. 1970 Dec;120(4):831–836. doi: 10.1042/bj1200831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Navarre D. A., Wolpert T. J. Inhibition of the glycine decarboxylase multienzyme complex by the host-selective toxin victorin. Plant Cell. 1995 Apr;7(4):463–471. doi: 10.1105/tpc.7.4.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Scofield SR, Tobias CM, Rathjen JP, Chang JH, Lavelle DT, Michelmore RW, Staskawicz BJ. Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of Tomato. Science. 1996 Dec 20;274(5295):2063–2065. doi: 10.1126/science.274.5295.2063. [DOI] [PubMed] [Google Scholar]
  13. Staskawicz B. J., Ausubel F. M., Baker B. J., Ellis J. G., Jones J. D. Molecular genetics of plant disease resistance. Science. 1995 May 5;268(5211):661–667. doi: 10.1126/science.7732374. [DOI] [PubMed] [Google Scholar]
  14. Tang X, Frederick RD, Zhou J, Halterman DA, Jia Y, Martin GB. Initiation of Plant Disease Resistance by Physical Interaction of AvrPto and Pto Kinase. Science. 1996 Dec 20;274(5295):2060–2063. doi: 10.1126/science.274.5295.2060. [DOI] [PubMed] [Google Scholar]
  15. Van den Ackerveken G., Marois E., Bonas U. Recognition of the bacterial avirulence protein AvrBs3 occurs inside the host plant cell. Cell. 1996 Dec 27;87(7):1307–1316. doi: 10.1016/s0092-8674(00)81825-5. [DOI] [PubMed] [Google Scholar]
  16. Yang Y., Gabriel D. W. Xanthomonas avirulence/pathogenicity gene family encodes functional plant nuclear targeting signals. Mol Plant Microbe Interact. 1995 Jul-Aug;8(4):627–631. doi: 10.1094/mpmi-8-0627. [DOI] [PubMed] [Google Scholar]
  17. Yoshikawa M., Keen N. T., Wang M. C. A receptor on soybean membranes for a fungal elicitor of phytoalexin accumulation. Plant Physiol. 1983 Oct;73(2):497–506. doi: 10.1104/pp.73.2.497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yucel I., Boyd C., Debnam Q., Keen N. T. Two different classes of avrD alleles occur in pathovars of Pseudomonas syringae. Mol Plant Microbe Interact. 1994 Jan-Feb;7(1):131–139. doi: 10.1094/mpmi-7-0131. [DOI] [PubMed] [Google Scholar]
  19. Yucel I., Midland S. L., Sims J. J., Keen N. T. Class I and class II avrD alleles direct the production of different products in gram-negative bacteria. Mol Plant Microbe Interact. 1994 Jan-Feb;7(1):148–150. doi: 10.1094/mpmi-7-0148. [DOI] [PubMed] [Google Scholar]

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