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. 1997 Dec;9(12):2119–2134. doi: 10.1105/tpc.9.12.2119

Regulatory sequences of Arabidopsis drive reporter gene expression in nematode feeding structures.

N Barthels 1, F M van der Lee 1, J Klap 1, O J Goddijn 1, M Karimi 1, P Puzio 1, F M Grundler 1, S A Ohl 1, K Lindsey 1, L Robertson 1, W M Robertson 1, M Van Montagu 1, G Gheysen 1, P C Sijmons 1
PMCID: PMC157062  PMID: 9437858

Abstract

In the quest for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures, we show that promoter tagging is a valuable tool. A large collection of transgenic Arabidopsis plants was generated. They were transformed with a beta-glucuronidase gene functioning as a promoter tag. Three T-DNA constructs, pGV1047, p delta gusBin19, and pMOG553, were used. Early responses to nematode invasion were of primary interest. Six lines exhibiting beta-glucuronidase activity in syncytia induced by the beet cyst nematode were studied. Reporter gene activation was also identified in galls induced by root knot and ectoparasitic nematodes. Time-course studies revealed that all six tags were differentially activated during the development of the feeding structure. T-DNA-flanking regions responsible for the observed responses after nematode infection were isolated and characterized for promoter activity.

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Selected References

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