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. 1997 Dec;9(12):2261–2270. doi: 10.1105/tpc.9.12.2261

Protein binding to the abscisic acid-responsive element is independent of VIVIPAROUS1 in vivo.

P K Busk 1, M Pagès 1
PMCID: PMC157072  PMID: 11407411

Abstract

The plant hormone abscisic acid and the transcriptional activator VIVIPAROUS1 have a synergistic effect on transcription during embryo development. An abscisic acid-responsive element (ABRE) mediates induction by abscisic acid and VIVIPAROUS1, but the mechanism involved has not been determined. In this study, we explore the interaction between abscisic acid and VIVIPAROUS1 and its effect on the ABRE from the maize rab28 gene. In transient transformation experiments, abscisic acid stimulated transcription via several elements, whereas activation by VIVIPAROUS1 was mediated exclusively through the ABRE. In vivo footprinting showed only minor differences in binding to the ABRE between wild-type and VIVIPAROUS1-deficient embryos, suggesting that VIVIPAROUS1 stimulates transcription through the ABRE without major changes in protein-DNA interactions. A factor that bound to the ABRE in electrophoretic mobility shift assays was present at the same developmental stages as rab28 mRNA and had binding characteristics similar to those observed by in vivo footprinting. This suggests that the factor binds to the ABRE in the rab28 promoter in vivo. We discuss the constraints that our results put on the possible mechanism for action of VIVIPAROUS1 in vivo.

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Selected References

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  1. Busk P. K., Jensen A. B., Pagès M. Regulatory elements in vivo in the promoter of the abscisic acid responsive gene rab17 from maize. Plant J. 1997 Jun;11(6):1285–1295. doi: 10.1046/j.1365-313x.1997.11061285.x. [DOI] [PubMed] [Google Scholar]
  2. Chern M. S., Bobb A. J., Bustos M. M. The regulator of MAT2 (ROM2) protein binds to early maturation promoters and represses PvALF-activated transcription. Plant Cell. 1996 Feb;8(2):305–321. doi: 10.1105/tpc.8.2.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Giuliano G., Pichersky E., Malik V. S., Timko M. P., Scolnik P. A., Cashmore A. R. An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7089–7093. doi: 10.1073/pnas.85.19.7089. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Guiltinan M. J., Marcotte W. R., Jr, Quatrano R. S. A plant leucine zipper protein that recognizes an abscisic acid response element. Science. 1990 Oct 12;250(4978):267–271. doi: 10.1126/science.2145628. [DOI] [PubMed] [Google Scholar]
  5. Hattori T., Terada T., Hamasuna S. Regulation of the Osem gene by abscisic acid and the transcriptional activator VP1: analysis of cis-acting promoter elements required for regulation by abscisic acid and VP1. Plant J. 1995 Jun;7(6):913–925. doi: 10.1046/j.1365-313x.1995.07060913.x. [DOI] [PubMed] [Google Scholar]
  6. Hill A., Nantel A., Rock C. D., Quatrano R. S. A conserved domain of the viviparous-1 gene product enhances the DNA binding activity of the bZIP protein EmBP-1 and other transcription factors. J Biol Chem. 1996 Feb 16;271(7):3366–3374. doi: 10.1074/jbc.271.7.3366. [DOI] [PubMed] [Google Scholar]
  7. Hughes D. W., Galau G. A. Developmental and environmental induction of Lea and LeaA mRNAs and the postabscission program during embryo culture. Plant Cell. 1991 Jun;3(6):605–618. doi: 10.1105/tpc.3.6.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ingram J., Bartels D. THE MOLECULAR BASIS OF DEHYDRATION TOLERANCE IN PLANTS. Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47(NaN):377–403. doi: 10.1146/annurev.arplant.47.1.377. [DOI] [PubMed] [Google Scholar]
  9. Izawa T., Foster R., Chua N. H. Plant bZIP protein DNA binding specificity. J Mol Biol. 1993 Apr 20;230(4):1131–1144. doi: 10.1006/jmbi.1993.1230. [DOI] [PubMed] [Google Scholar]
  10. Knetsch MLW., Wang M., Snaar-Jagalska B. E., Heimovaara-Dijkstra S. Abscisic Acid Induces Mitogen-Activated Protein Kinase Activation in Barley Aleurone Protoplasts. Plant Cell. 1996 Jun;8(6):1061–1067. doi: 10.1105/tpc.8.6.1061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leung J., Bouvier-Durand M., Morris P. C., Guerrier D., Chefdor F., Giraudat J. Arabidopsis ABA response gene ABI1: features of a calcium-modulated protein phosphatase. Science. 1994 Jun 3;264(5164):1448–1452. doi: 10.1126/science.7910981. [DOI] [PubMed] [Google Scholar]
  12. Lu G., Paul A. L., McCarty D. R., Ferl R. J. Transcription factor veracity: is GBF3 responsible for ABA-regulated expression of Arabidopsis Adh? Plant Cell. 1996 May;8(5):847–857. doi: 10.1105/tpc.8.5.847. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Marcotte W. R., Jr, Russell S. H., Quatrano R. S. Abscisic acid-responsive sequences from the em gene of wheat. Plant Cell. 1989 Oct;1(10):969–976. doi: 10.1105/tpc.1.10.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McCarty D. R., Hattori T., Carson C. B., Vasil V., Lazar M., Vasil I. K. The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator. Cell. 1991 Sep 6;66(5):895–905. doi: 10.1016/0092-8674(91)90436-3. [DOI] [PubMed] [Google Scholar]
  15. Meyer K., Leube M. P., Grill E. A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana. Science. 1994 Jun 3;264(5164):1452–1455. doi: 10.1126/science.8197457. [DOI] [PubMed] [Google Scholar]
  16. Niogret M. F., Culiáez-Macià F. A., Goday A., Mar Albà M., Pagès M. Expression and cellular localization of rab28 mRNA and Rab28 protein during maize embryogenesis. Plant J. 1996 Apr;9(4):549–557. doi: 10.1046/j.1365-313x.1996.09040549.x. [DOI] [PubMed] [Google Scholar]
  17. Niu X., Adams C. C., Workman J. L., Guiltinan M. J. Binding of the wheat basic leucine zipper protein EmBP-1 to nucleosomal binding sites is modulated by nucleosome positioning. Plant Cell. 1996 Sep;8(9):1569–1587. doi: 10.1105/tpc.8.9.1569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Niu X., Guiltinan M. J. DNA binding specificity of the wheat bZIP protein EmBP-1. Nucleic Acids Res. 1994 Nov 25;22(23):4969–4978. doi: 10.1093/nar/22.23.4969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Parcy F., Valon C., Raynal M., Gaubier-Comella P., Delseny M., Giraudat J. Regulation of gene expression programs during Arabidopsis seed development: roles of the ABI3 locus and of endogenous abscisic acid. Plant Cell. 1994 Nov;6(11):1567–1582. doi: 10.1105/tpc.6.11.1567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pla M., Gómez J., Goday A., Pagès M. Regulation of the abscisic acid-responsive gene rab28 in maize viviparous mutants. Mol Gen Genet. 1991 Dec;230(3):394–400. doi: 10.1007/BF00280296. [DOI] [PubMed] [Google Scholar]
  21. Pla M., Vilardell J., Guiltinan M. J., Marcotte W. R., Niogret M. F., Quatrano R. S., Pagès M. The cis-regulatory element CCACGTGG is involved in ABA and water-stress responses of the maize gene rab28. Plant Mol Biol. 1993 Jan;21(2):259–266. doi: 10.1007/BF00019942. [DOI] [PubMed] [Google Scholar]
  22. Rigaud G., Roux J., Pictet R., Grange T. In vivo footprinting of rat TAT gene: dynamic interplay between the glucocorticoid receptor and a liver-specific factor. Cell. 1991 Nov 29;67(5):977–986. doi: 10.1016/0092-8674(91)90370-e. [DOI] [PubMed] [Google Scholar]
  23. Salinas J., Oeda K., Chua N. H. Two G-box-related sequences confer different expression patterns in transgenic tobacco. Plant Cell. 1992 Dec;4(12):1485–1493. doi: 10.1105/tpc.4.12.1485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schultz T. F., Spiker S., Quatrano R. S. Histone H1 enhances the DNA binding activity of the transcription factor EmBP-1. J Biol Chem. 1996 Oct 18;271(42):25742–25745. doi: 10.1074/jbc.271.42.25742. [DOI] [PubMed] [Google Scholar]
  25. Shen Q., Ho T. H. Functional dissection of an abscisic acid (ABA)-inducible gene reveals two independent ABA-responsive complexes each containing a G-box and a novel cis-acting element. Plant Cell. 1995 Mar;7(3):295–307. doi: 10.1105/tpc.7.3.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shen Q., Zhang P., Ho T. H. Modular nature of abscisic acid (ABA) response complexes: composite promoter units that are necessary and sufficient for ABA induction of gene expression in barley. Plant Cell. 1996 Jul;8(7):1107–1119. doi: 10.1105/tpc.8.7.1107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Suzuki M., Kao C. Y., McCarty D. R. The conserved B3 domain of VIVIPAROUS1 has a cooperative DNA binding activity. Plant Cell. 1997 May;9(5):799–807. doi: 10.1105/tpc.9.5.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vasil V., Marcotte W. R., Jr, Rosenkrans L., Cocciolone S. M., Vasil I. K., Quatrano R. S., McCarty D. R. Overlap of Viviparous1 (VP1) and abscisic acid response elements in the Em promoter: G-box elements are sufficient but not necessary for VP1 transactivation. Plant Cell. 1995 Sep;7(9):1511–1518. doi: 10.1105/tpc.7.9.1511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Vilardell J., Goday A., Freire M. A., Torrent M., Martínez M. C., Torné J. M., Pagès M. Gene sequence, developmental expression, and protein phosphorylation of RAB-17 in maize. Plant Mol Biol. 1990 Mar;14(3):423–432. doi: 10.1007/BF00028778. [DOI] [PubMed] [Google Scholar]
  30. Williamson J. D., Scandalios J. G. Differential response of maize catalases to abscisic acid: Vp1 transcriptional activator is not required for abscisic acid-regulated Cat1 expression. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8842–8846. doi: 10.1073/pnas.89.18.8842. [DOI] [PMC free article] [PubMed] [Google Scholar]

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