Abstract
A recombinant phage containing an Arabidopsis thaliana cDNA sequence encoding a protein with 1L-myo-inositol 1-phosphate synthase (EC 5.5.1.4) activity has been isolated and used for transcriptional and translational studies. The identification of the recombinant phage relied on the observations that (a) the clone complements a mutation in the structural gene for 1L-myo-inositol 1-phosphate synthase in the yeast Saccharomyces cerevisiae, (b) the in vitro synthesized polypeptide enzymatically converts glucose 6-phosphate into inositol 1-phosphate, (c) in vitro transcription and translation of this cDNA sequence produces a polypeptide that is recognized by anti-yeast myo-inositol 1-phosphate synthase antiserum, and (d) inositol regulates the expression of the corresponding gene in Arabidopsis.
Full Text
The Full Text of this article is available as a PDF (1.6 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Berridge M. J. Inositol trisphosphate and calcium signalling. Nature. 1993 Jan 28;361(6410):315–325. doi: 10.1038/361315a0. [DOI] [PubMed] [Google Scholar]
- Berridge M. J., Irvine R. F. Inositol phosphates and cell signalling. Nature. 1989 Sep 21;341(6239):197–205. doi: 10.1038/341197a0. [DOI] [PubMed] [Google Scholar]
- Berridge M. V., Lane C. D. Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins. Cell. 1976 Jun;8(2):283–297. doi: 10.1016/0092-8674(76)90012-x. [DOI] [PubMed] [Google Scholar]
- Burgers P. M., Percival K. J. Transformation of yeast spheroplasts without cell fusion. Anal Biochem. 1987 Jun;163(2):391–397. doi: 10.1016/0003-2697(87)90240-5. [DOI] [PubMed] [Google Scholar]
- Chen I. W., Charalampous C. F. Biochemical studies on inositol. IX. D-Inositol 1-phosphate as intermediate in the biosynthesis of inositol from glucose 6-phosphate, and characteristics of two reactions in this biosynthesis. J Biol Chem. 1966 May 25;241(10):2194–2199. [PubMed] [Google Scholar]
- Culbertson M. R., Donahue T. F., Henry S. A. Control of inositol biosynthesis in Saccharomyces cerevisiae: properties of a repressible enzyme system in extracts of wild-type (Ino+) cells. J Bacteriol. 1976 Apr;126(1):232–242. doi: 10.1128/jb.126.1.232-242.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dean-Johnson M., Henry S. A. Biosynthesis of inositol in yeast. Primary structure of myo-inositol-1-phosphate synthase (EC 5.5.1.4) and functional analysis of its structural gene, the INO1 locus. J Biol Chem. 1989 Jan 15;264(2):1274–1283. [PubMed] [Google Scholar]
- Donahue T. F., Henry S. A. myo-Inositol-1-phosphate synthase. Characteristics of the enzyme and identification of its structural gene in yeast. J Biol Chem. 1981 Jul 10;256(13):7077–7085. [PubMed] [Google Scholar]
- Elledge S. J., Mulligan J. T., Ramer S. W., Spottswood M., Davis R. W. Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1731–1735. doi: 10.1073/pnas.88.5.1731. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hokin L. E. Receptors and phosphoinositide-generated second messengers. Annu Rev Biochem. 1985;54:205–235. doi: 10.1146/annurev.bi.54.070185.001225. [DOI] [PubMed] [Google Scholar]
- Johnson M. D. The arabidopsis thaliana myo-inositol 1-phosphate synthase (EC 5.5.1.4). Plant Physiol. 1994 Jul;105(3):1023–1024. doi: 10.1104/pp.105.3.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kiely D. E., Sherman W. R. A chemical model for the cyclization step in the biosynthesis of L-myo-inositol 1-phosphate. J Am Chem Soc. 1975 Nov 12;97(23):6810–6814. doi: 10.1021/ja00856a035. [DOI] [PubMed] [Google Scholar]
- Klig L. S., Henry S. A. Isolation of the yeast INO1 gene: located on an autonomously replicating plasmid, the gene is fully regulated. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3816–3820. doi: 10.1073/pnas.81.12.3816. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOEWUS F. A., KELLY S. Conversion of glucose to inositol in parsley leaves. Biochem Biophys Res Commun. 1962 Apr 20;7:204–208. doi: 10.1016/0006-291x(62)90175-4. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Loewus M. W., Loewus F. The Isolation and Characterization of d-Glucose 6-Phosphate Cycloaldolase (NAD-Dependent) from Acer pseudoplatanus L. Cell Cultures: Its Occurrence in Plants. Plant Physiol. 1971 Sep;48(3):255–260. doi: 10.1104/pp.48.3.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loewy B. S., Henry S. A. The INO2 and INO4 loci of Saccharomyces cerevisiae are pleiotropic regulatory genes. Mol Cell Biol. 1984 Nov;4(11):2479–2485. doi: 10.1128/mcb.4.11.2479. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maeda T., Eisenberg F., Jr Purification, structure, and catalytic properties of L-myo-inositol-1-phosphate synthase from rat testis. J Biol Chem. 1980 Sep 25;255(18):8458–8464. [PubMed] [Google Scholar]
- Meinke D. W., Sussex I. M. Embryo-lethal mutants of Arabidopsis thaliana. A model system for genetic analysis of plant embryo development. Dev Biol. 1979 Sep;72(1):50–61. doi: 10.1016/0012-1606(79)90097-6. [DOI] [PubMed] [Google Scholar]
- Morse M. J., Crain R. C., Satter R. L. Phosphatidylinositol Cycle Metabolites in Samanea saman Pulvini. Plant Physiol. 1987 Mar;83(3):640–644. doi: 10.1104/pp.83.3.640. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakanishi T., Turner R. J., Burg M. B. Osmoregulatory changes in myo-inositol transport by renal cells. Proc Natl Acad Sci U S A. 1989 Aug;86(15):6002–6006. doi: 10.1073/pnas.86.15.6002. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ogunyemi E. O., Pittner F., Hoffmann-Ostenhof O. Studies on the biosynthesis of cyclitols, XXXVI. Purification of myo-inositol-1-phosphate synthase of the duckweed, Lemna gibba, to homogeneity by affinity chromatography on NAD-Sepharose molecular and catalytic properties of the enzyme. Hoppe Seylers Z Physiol Chem. 1978 May;359(5):613–616. [PubMed] [Google Scholar]
- Robzyk K., Kassir Y. A simple and highly efficient procedure for rescuing autonomous plasmids from yeast. Nucleic Acids Res. 1992 Jul 25;20(14):3790–3790. doi: 10.1093/nar/20.14.3790. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sacher R. F., Staples R. C. Inositol and sugars in adaptation of tomato to salt. Plant Physiol. 1985 Jan;77(1):206–210. doi: 10.1104/pp.77.1.206. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sherman W. R., Rasheed A., Mauck L. A., Wiecko J. Incubations of testis myo-inositol-1-phosphate synthase with D-(5-18O)glucose 6-phosphate and with H218O show no evidence of Schiff base formation. J Biol Chem. 1977 Aug 25;252(16):5672–5676. [PubMed] [Google Scholar]
- Smart C. C., Fleming A. J. A plant gene with homology to D-myo-inositol-3-phosphate synthase is rapidly and spatially up-regulated during an abscisic-acid-induced morphogenic response in Spirodela polyrrhiza. Plant J. 1993 Aug;4(2):279–293. doi: 10.1046/j.1365-313x.1993.04020279.x. [DOI] [PubMed] [Google Scholar]
- TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
- Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wong Y. H., Sherman W. R. Anomeric and other substrate specificity studies with myo-inositol-1-P synthase. J Biol Chem. 1985 Sep 15;260(20):11083–11090. [PubMed] [Google Scholar]
- des Francs C. C., Thiellement H., de Vienne D. Analysis of Leaf Proteins by Two-Dimensional Gel Electrophoresis: Protease Action as Exemplified by Ribulose Bisphosphate Carboxylase/ Oxygenase Degradation and Procedure to Avoid Proteolysis during Extraction. Plant Physiol. 1985 May;78(1):178–182. doi: 10.1104/pp.78.1.178. [DOI] [PMC free article] [PubMed] [Google Scholar]