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Plant Physiology logoLink to Plant Physiology
. 1995 Feb;107(2):613–619. doi: 10.1104/pp.107.2.613

1 L-myo-Inositol 1-Phosphate Synthase from Arabidopsis thaliana.

M D Johnson 1, I M Sussex 1
PMCID: PMC157165  PMID: 12228386

Abstract

A recombinant phage containing an Arabidopsis thaliana cDNA sequence encoding a protein with 1L-myo-inositol 1-phosphate synthase (EC 5.5.1.4) activity has been isolated and used for transcriptional and translational studies. The identification of the recombinant phage relied on the observations that (a) the clone complements a mutation in the structural gene for 1L-myo-inositol 1-phosphate synthase in the yeast Saccharomyces cerevisiae, (b) the in vitro synthesized polypeptide enzymatically converts glucose 6-phosphate into inositol 1-phosphate, (c) in vitro transcription and translation of this cDNA sequence produces a polypeptide that is recognized by anti-yeast myo-inositol 1-phosphate synthase antiserum, and (d) inositol regulates the expression of the corresponding gene in Arabidopsis.

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Selected References

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