Abstract
Three nuclear localization signals (NLS), including an unusual Mat alpha 2-like NLS from maize (Zea mays) R, were found to compete for binding to plant nuclei. In addition, the authentic yeast Mat alpha 2 NLS, which does not function in mammals, was shown to function in plants in vivo. Our results indicate that plants possess a site at the nuclear pore complex that recognizes the three known classes of NLSs.
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Selected References
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- Carrington J. C., Freed D. D., Leinicke A. J. Bipartite signal sequence mediates nuclear translocation of the plant potyviral NIa protein. Plant Cell. 1991 Sep;3(9):953–962. doi: 10.1105/tpc.3.9.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chelsky D., Ralph R., Jonak G. Sequence requirements for synthetic peptide-mediated translocation to the nucleus. Mol Cell Biol. 1989 Jun;9(6):2487–2492. doi: 10.1128/mcb.9.6.2487. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Citovsky V., Zambryski P. Transport of nucleic acids through membrane channels: snaking through small holes. Annu Rev Microbiol. 1993;47:167–197. doi: 10.1146/annurev.mi.47.100193.001123. [DOI] [PubMed] [Google Scholar]
- Dombrowski J. E., Schroeder M. R., Bednarek S. Y., Raikhel N. V. Determination of the functional elements within the vacuolar targeting signal of barley lectin. Plant Cell. 1993 May;5(5):587–596. doi: 10.1105/tpc.5.5.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forbes D. J. Structure and function of the nuclear pore complex. Annu Rev Cell Biol. 1992;8:495–527. doi: 10.1146/annurev.cb.08.110192.002431. [DOI] [PubMed] [Google Scholar]
- Garcia-Bustos J., Heitman J., Hall M. N. Nuclear protein localization. Biochim Biophys Acta. 1991 Mar 7;1071(1):83–101. doi: 10.1016/0304-4157(91)90013-m. [DOI] [PubMed] [Google Scholar]
- Harter K., Kircher S., Frohnmeyer H., Krenz M., Nagy F., Schäfer E. Light-regulated modification and nuclear translocation of cytosolic G-box binding factors in parsley. Plant Cell. 1994 Apr;6(4):545–559. doi: 10.1105/tpc.6.4.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hicks G. R., Raikhel N. V. Specific binding of nuclear localization sequences to plant nuclei. Plant Cell. 1993 Aug;5(8):983–994. doi: 10.1105/tpc.5.8.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moore M. S., Blobel G. A G protein involved in nucleocytoplasmic transport: the role of Ran. Trends Biochem Sci. 1994 May;19(5):211–216. doi: 10.1016/0968-0004(94)90024-8. [DOI] [PubMed] [Google Scholar]
- Shieh M. W., Wessler S. R., Raikhel N. V. Nuclear targeting of the maize R protein requires two nuclear localization sequences. Plant Physiol. 1993 Feb;101(2):353–361. doi: 10.1104/pp.101.2.353. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Varagona M. J., Raikhel N. V. The basic domain in the bZIP regulatory protein Opaque2 serves two independent functions: DNA binding and nuclear localization. Plant J. 1994 Feb;5(2):207–214. doi: 10.1046/j.1365-313x.1994.05020207.x. [DOI] [PubMed] [Google Scholar]
- Varagona M. J., Schmidt R. J., Raikhel N. V. Nuclear localization signal(s) required for nuclear targeting of the maize regulatory protein Opaque-2. Plant Cell. 1992 Oct;4(10):1213–1227. doi: 10.1105/tpc.4.10.1213. [DOI] [PMC free article] [PubMed] [Google Scholar]