Abstract
Rates of C2H2 reduction and CO2 evolution by nodules were measured in a flowthrough system using intact plants of Myrica gale L. Both activities increased linearly with increasing partial pressure of O2 (pO2) up to 18 kPa. The linear relationship between CO2 evolution and pO2 at pO2 values between 6 and 18 kPa suggests that the diffusion barrier has a constant resistance. The lack of a variable resistance was further supported by sustained increases and decreases in nodule activities in response to changes in pO2 in the range of 6 to 20 kPa O2. When pO2 was increased above 20 kPa, C2H2 reduction and CO2 evolution continually declined with time. These results confirm that the diffusion barrier in nodules of M. gale is not variable in response to changes in pO2. The effect of temperature was examined at 8 and 20 kPa O2. Rates of C2H2 reduction and CO2 evolution increased with increasing temperature from 10 to 30[deg]C at both pO2 values. These results indicate that the diffusion resistance of the barrier changes as temperature changes, with the resistance decreasing as temperature increases.
Full Text
The Full Text of this article is available as a PDF (786.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Monroe J. D., Larue T. A. Effects of carbohydrate on the internal oxygen concentration, oxygen uptake, and nitrogenase activity in detached pea nodules. Plant Physiol. 1989 Oct;91(2):603–609. doi: 10.1104/pp.91.2.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Silvester W. B., Winship L. J. Transient responses of nitrogenase to acetylene and oxygen in actinorhizal nodules and cultured frankia. Plant Physiol. 1990 Feb;92(2):480–486. doi: 10.1104/pp.92.2.480. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tjepkema J. D., Schwintzer C. R. Factors Affecting the Acetylene-Induced Decline during Nitrogenase Assays in Root Nodules of Myrica gale L. Plant Physiol. 1992 Apr;98(4):1451–1459. doi: 10.1104/pp.98.4.1451. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tjepkema J. D., Schwintzer C. R., Monz C. A. Time course of acetylene reduction in nodules of five actinorhizal genera. Plant Physiol. 1988 Feb;86(2):581–583. doi: 10.1104/pp.86.2.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weisz P. R., Sinclair T. R. Regulation of Soybean Nitrogen Fixation in Response to Rhizosphere Oxygen: II. Quantification of Nodule Gas Permeability. Plant Physiol. 1987 Jul;84(3):906–910. doi: 10.1104/pp.84.3.906. [DOI] [PMC free article] [PubMed] [Google Scholar]
