Abstract
Fructose-2,6-bisphosphate (Fru-2,6-bisP) was evaluated as a potential marker for the dormancy-breaking phase or the germination phase before pericarp splitting in red rice (Oryza sativa). During 4 h of imbibition at 30[deg]C, Fru-2,6-bisP of dehulled dormant and nondormant seeds increased to 0.26 and 0.38 pmol embryo-1, respectively. In nondormant seeds, embryo Fru-2,6-bisP content remained stable until the onset of pericarp splitting (12 h) and increased rapidly thereafter. In dormant seeds, Fru-2,6-bisP declined to 0.09 pmol embryo-1 at 24 h. Embryo Fru-2,6-bisP was correlated with O2 uptake of dormant and nondormant seeds. A 24-h exposure of dehulled, water-imbibed, dormant seeds to treatments yielding >90% germination (sodium nitrite [4 mM], propionic acid [22 mM], methyl propionate [32 mM], propanol [75 mM], and propionaldehyde [40 mM]) led to changes in embryo Fru-2,6-bisP that were unrelated to the final germination percentages. Furthermore, a 2-h pulse of propionaldehyde increased Fru-2,6-bisP 4-fold but did not break dormancy. Whereas nitrite and propionaldehyde increased Fru-2,6-bisP to 0.33 pmol embryo-1 after 2 h of contact, propionic acid and methyl propionate did not increase Fru-2,6-bisP above the untreated control. In all cases, further increases in Fru-2,6-bisP occurred after pericarp splitting. However, the plateau Fru-2,6-bisP attained during chemical contact was inversely correlated with elapsed time to 30% germination (r = -0.978). Therefore, although Fru-2,6-bisP is not a universal marker for dormancy release, its rapid increase during nitrite and propionaldehyde treatments suggests that events associated with dormancy breaking can occur within 2 h of chemical treatment.
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- Busa W. B. Mechanisms and consequences of pH-mediated cell regulation. Annu Rev Physiol. 1986;48:389–402. doi: 10.1146/annurev.ph.48.030186.002133. [DOI] [PubMed] [Google Scholar]
- Epel D. The initiation of development at fertilization. Cell Differ Dev. 1990 Jan;29(1):1–12. doi: 10.1016/0922-3371(90)90019-s. [DOI] [PubMed] [Google Scholar]
- Footitt S., Cohn M. A. Seed Dormancy in Red Rice : VIII. Embryo Acidification during Dormancy-Breaking and Subsequent Germination. Plant Physiol. 1992 Nov;100(3):1196–1202. doi: 10.1104/pp.100.3.1196. [DOI] [PMC free article] [PubMed] [Google Scholar]
- François J., Van Schaftingen E., Hers H. G. Effect of benzoate on the metabolism of fructose 2,6-bisphosphate in yeast. Eur J Biochem. 1986 Jan 2;154(1):141–145. doi: 10.1111/j.1432-1033.1986.tb09369.x. [DOI] [PubMed] [Google Scholar]
- François J., Villanueva M. E., Hers H. G. The control of glycogen metabolism in yeast. 1. Interconversion in vivo of glycogen synthase and glycogen phosphorylase induced by glucose, a nitrogen source or uncouplers. Eur J Biochem. 1988 Jun 15;174(3):551–559. doi: 10.1111/j.1432-1033.1988.tb14134.x. [DOI] [PubMed] [Google Scholar]
- Halinska A., Frenkel C. Acetaldehyde stimulation of net gluconeogenic carbon movement from applied malic Acid in tomato fruit pericarp tissue. Plant Physiol. 1991 Mar;95(3):954–960. doi: 10.1104/pp.95.3.954. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hue L., Maisin L., Rider M. H. Palmitate inhibits liver glycolysis. Involvement of fructose 2,6-bisphosphate in the glucose/fatty acid cycle. Biochem J. 1988 Apr 15;251(2):541–545. doi: 10.1042/bj2510541. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Larondelle Y., Corbineau F., Dethier M., Come D., Hers H. G. Fructose 2,6-bisphosphate in germinating oat seeds. A biochemical study of seed dormancy. Eur J Biochem. 1987 Aug 3;166(3):605–610. doi: 10.1111/j.1432-1033.1987.tb13556.x. [DOI] [PubMed] [Google Scholar]
- Leprince O., Atherton N. M., Deltour R., Hendry GAF. The Involvement of Respiration in Free Radical Processes during Loss of Desiccation Tolerance in Germinating Zea mays L. (An Electron Paramagnetic Resonance Study). Plant Physiol. 1994 Apr;104(4):1333–1339. doi: 10.1104/pp.104.4.1333. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mertens E., Larondelle Y., Hers H. G. Induction of pyrophosphate:fructose 6-phosphate 1-phosphotransferase by anoxia in rice seedlings. Plant Physiol. 1990 Jun;93(2):584–587. doi: 10.1104/pp.93.2.584. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Laere A., Van Schaftingen E., Hers H. G. Fructose 2,6-bisphosphate and germination of fungal spores. Proc Natl Acad Sci U S A. 1983 Nov;80(21):6601–6605. doi: 10.1073/pnas.80.21.6601. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Schaftingen E., Bartrons R., Hers H. G. The mechanism by which ethanol decreases the concentration of fructose 2,6-bisphosphate in the liver. Biochem J. 1984 Sep 1;222(2):511–518. doi: 10.1042/bj2220511. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Schaftingen E. Fructose 2,6-bisphosphate. Adv Enzymol Relat Areas Mol Biol. 1987;59:315–395. doi: 10.1002/9780470123058.ch7. [DOI] [PubMed] [Google Scholar]
- Warth A. D. Effect of benzoic acid on glycolytic metabolite levels and intracellular pH in Saccharomyces cerevisiae. Appl Environ Microbiol. 1991 Dec;57(12):3415–3417. doi: 10.1128/aem.57.12.3415-3417.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]