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. 1995 May;108(1):99–103. doi: 10.1104/pp.108.1.99

Subcellular Compartmentation of the 4-Aminobutyrate Shunt in Protoplasts from Developing Soybean Cotyledons.

K E Breitkreuz 1, B J Shelp 1
PMCID: PMC157309  PMID: 12228455

Abstract

The subcellular localization of enzymes involved in the 4-ami-nobutyrate shunt was investigated in protoplasts prepared from developing soybean [Glycine max (L.) Merrill cv Maple Arrow] cotyledons. Protoplast lysate was fractionated by differential and continuous Percoll-gradient centrifugation to separate organelle fractions. Glutamate decarboxylase (EC 4.1.1.15) was found exclusively in the cytosol, whereas 4-aminobutyrate:pyruvate transami-nase (EC 2.6.1.19) and succinic semialdehyde dehydrogenase (EC 1.2.1.16) were associated exclusively with the mitochondrial fractions. Mitochondrial fractions also catabolized [U-14C]4-aminobu-tyrate to labeled succinate.

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Selected References

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  1. Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121–126. doi: 10.1016/s0076-6879(84)05016-3. [DOI] [PubMed] [Google Scholar]
  2. Baum G., Chen Y., Arazi T., Takatsuji H., Fromm H. A plant glutamate decarboxylase containing a calmodulin binding domain. Cloning, sequence, and functional analysis. J Biol Chem. 1993 Sep 15;268(26):19610–19617. [PubMed] [Google Scholar]
  3. Bergman A., Gardeström P., Ericson I. Method to Obtain a Chlorophyll-free Preparation of Intact Mitochondria from Spinach Leaves. Plant Physiol. 1980 Sep;66(3):442–445. doi: 10.1104/pp.66.3.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Good A. G., Muench D. G. Purification and characterization of an anaerobically induced alanine aminotransferase from barley roots. Plant Physiol. 1992 Aug;99(4):1520–1525. doi: 10.1104/pp.99.4.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hearl W. G., Churchich J. E. Interactions between 4-aminobutyrate aminotransferase and succinic semialdehyde dehydrogenase, two mitochondrial enzymes. J Biol Chem. 1984 Sep 25;259(18):11459–11463. [PubMed] [Google Scholar]
  6. John R. A., Charteris A. The reaction of amino-oxyacetate with pyridoxal phosphate-dependent enzymes. Biochem J. 1978 Jun 1;171(3):771–779. doi: 10.1042/bj1710771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Journet E. P., Bonner W. D., Douce R. Glutamate metabolism triggered by oxaloacetate in intact plant mitochondria. Arch Biochem Biophys. 1982 Mar;214(1):366–375. doi: 10.1016/0003-9861(82)90041-8. [DOI] [PubMed] [Google Scholar]
  8. Kimmerer T. W. Alcohol Dehydrogenase and Pyruvate Decarboxylase Activity in Leaves and Roots of Eastern Cottonwood (Populus deltoides Bartr.) and Soybean (Glycine max L.). Plant Physiol. 1987 Aug;84(4):1210–1213. doi: 10.1104/pp.84.4.1210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ling V., Snedden W. A., Shelp B. J., Assmann S. M. Analysis of a soluble calmodulin binding protein from fava bean roots: identification of glutamate decarboxylase as a calmodulin-activated enzyme. Plant Cell. 1994 Aug;6(8):1135–1143. doi: 10.1105/tpc.6.8.1135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schnarrenberger C., Oeser A., Tolbert N. E. Development of Microbodies in Sunflower Cotyledons and Castor Bean Endosperm during Germination. Plant Physiol. 1971 Nov;48(5):566–574. doi: 10.1104/pp.48.5.566. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Snedden W. A., Chung I., Pauls R. H., Bown A. W. Proton/l-Glutamate Symport and the Regulation of Intracellular pH in Isolated Mesophyll Cells. Plant Physiol. 1992 Jun;99(2):665–671. doi: 10.1104/pp.99.2.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Streeter J. G., Thompson J. F. In Vivo and In Vitro Studies on gamma-Aminobutyric Acid Metabolism with the Radish Plant (Raphanus sativus, L.). Plant Physiol. 1972 Apr;49(4):579–584. doi: 10.1104/pp.49.4.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wallace W., Secor J., Schrader L. E. Rapid Accumulation of gamma-Aminobutyric Acid and Alanine in Soybean Leaves in Response to an Abrupt Transfer to Lower Temperature, Darkness, or Mechanical Manipulation. Plant Physiol. 1984 May;75(1):170–175. doi: 10.1104/pp.75.1.170. [DOI] [PMC free article] [PubMed] [Google Scholar]

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