Abstract
Changes in gene expression and tissue composition were investigated during foliar development and natural senescence of asparagus (Asparagus officinalis L.). Three phases in development and senescence of the foliage were characterized: early fern growth, mature fern, and senescence, when a marked loss of chlorophyll, sucrose, and protein occurred and major changes in translatable mRNAs were detected. Transcripts for three asparagus spear harvest-induced cDNA clones, pTIP9, pTIP11, and pTIP12 (G.A. King and K.M. Davies [1992] Plant Physiol 100: 1661-1669), accumulated during natural foliar senescence, suggesting that the underlying regulatory mechanisms may be similar in both developmental situations. We have used our knowledge of asparagus spear physiology, the probable proteins encoded by the cDNA clones, and our fern development data to propose that sugar depletion regulates the accumulation of at least pTIP12 transcripts in senescing asparagus tissue.
Full Text
The Full Text of this article is available as a PDF (969.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Biggs M. S., Harriman R. W., Handa A. K. Changes in Gene Expression during Tomato Fruit Ripening. Plant Physiol. 1986 Jun;81(2):395–403. doi: 10.1104/pp.81.2.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davies K. M., King G. A. Isolation and characterization of a cDNA clone for a harvest-induced asparagine synthetase from Asparagus officinalis L. Plant Physiol. 1993 Aug;102(4):1337–1340. doi: 10.1104/pp.102.4.1337. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Genix P., Bligny R., Martin J. B., Douce R. Transient accumulation of asparagine in sycamore cells after a long period of sucrose starvation. Plant Physiol. 1990 Oct;94(2):717–722. doi: 10.1104/pp.94.2.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Inskeep W. P., Bloom P. R. Extinction coefficients of chlorophyll a and B in n,n-dimethylformamide and 80% acetone. Plant Physiol. 1985 Feb;77(2):483–485. doi: 10.1104/pp.77.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iusem N. D., Bartholomew D. M., Hitz W. D., Scolnik P. A. Tomato (Lycopersicon esculentum) transcript induced by water deficit and ripening. Plant Physiol. 1993 Aug;102(4):1353–1354. doi: 10.1104/pp.102.4.1353. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kamachi K., Yamaya T., Mae T., Ojima K. A Role for Glutamine Synthetase in the Remobilization of Leaf Nitrogen during Natural Senescence in Rice Leaves. Plant Physiol. 1991 Jun;96(2):411–417. doi: 10.1104/pp.96.2.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
- King G. A., Davies K. M. Cloning of a harvest-induced beta-galactosidase from tips of harvested asparagus spears. Plant Physiol. 1995 May;108(1):419–420. doi: 10.1104/pp.108.1.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
- King G. A., Davies K. M. Identification, cDNA Cloning, and Analysis of mRNAs Having Altered Expression in Tips of Harvested Asparagus Spears. Plant Physiol. 1992 Dec;100(4):1661–1669. doi: 10.1104/pp.100.4.1661. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moran R., Porath D. Chlorophyll determination in intact tissues using n,n-dimethylformamide. Plant Physiol. 1980 Mar;65(3):478–479. doi: 10.1104/pp.65.3.478. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Redgwell R. J., Melton L. D., Brasch D. J. Cell Wall Dissolution in Ripening Kiwifruit (Actinidia deliciosa) : Solubilization of the Pectic Polymers. Plant Physiol. 1992 Jan;98(1):71–81. doi: 10.1104/pp.98.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tsai F. Y., Coruzzi G. Light represses transcription of asparagine synthetase genes in photosynthetic and nonphotosynthetic organs of plants. Mol Cell Biol. 1991 Oct;11(10):4966–4972. doi: 10.1128/mcb.11.10.4966. [DOI] [PMC free article] [PubMed] [Google Scholar]