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. 1995 May;108(1):277–283. doi: 10.1104/pp.108.1.277

Measurements of in Vivo Ubiquinone Reduction Levels in Plant Cells.

A M Wagner 1, M J Wagner 1
PMCID: PMC157332  PMID: 12228473

Abstract

A method is described for the determination of in vivo ubiquinone (UQ) reduction levels in nongreen tissues by extraction and subsequent detection of ubiquinone-10 and ubiquinol-10 with high-performance liquid chromatography. In Petunia hybrida cell suspensions UQ reduction remained at a stable level of about 60%, despite the changing conditions during the batch culture (from excess sugar to starvation) and the concomitant variations in respiration. Also, in the presence of uncoupler, which causes a large increase in respiration via both the cytochrome pathway and the alternative pathway, UQ reduction levels stayed at 60%. In mitochondria isolated from these cells, activity of the alternative pathway was only observed at UQ reduction levels higher than 80%. It is proposed that in vivo the relationship between UQ reduction and the activity of the alternative oxidase is modulated by mechanisms such as thiol modifications and accumulation of organic acids. Accordingly, pyruvate concentration in P. hybrida cells increased in the presence of uncoupler.

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Selected References

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  1. Bahr J. T., Bonner W. D., Jr Cyanide-insensitive respiration. I. The steady states of skunk cabbage spadix and bean hypocotyl mitochondria. J Biol Chem. 1973 May 25;248(10):3441–3445. [PubMed] [Google Scholar]
  2. Bahr J. T., Bonner W. D., Jr Cyanide-insensitive respiration. II. Control of the alternate pathway. J Biol Chem. 1973 May 25;248(10):3446–3450. [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  4. Couée I., Jan M., Carde J. P., Brouquisse R., Raymond P., Pradet A. Effects of glucose starvation on mitochondrial subpopulations in the meristematic and submeristematic regions of maize root. Plant Physiol. 1992 Dec;100(4):1891–1900. doi: 10.1104/pp.100.4.1891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Day D. A., Dry I. B., Soole K. L., Wiskich J. T., Moore A. L. Regulation of Alternative Pathway Activity in Plant Mitochondria : Deviations from Q-Pool Behavior during Oxidation of NADH and Quinols. Plant Physiol. 1991 Mar;95(3):948–953. doi: 10.1104/pp.95.3.948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dry I. B., Moore A. L., Day D. A., Wiskich J. T. Regulation of alternative pathway activity in plant mitochondria: nonlinear relationship between electron flux and the redox poise of the quinone pool. Arch Biochem Biophys. 1989 Aug 15;273(1):148–157. doi: 10.1016/0003-9861(89)90173-2. [DOI] [PubMed] [Google Scholar]
  7. Hemrika-Wagner A. M., Kreuk K. C., van der Plas L. H. Influence of growth temperature on respiratory characteristics of mitochondria from callus-forming potato tuber discs. Plant Physiol. 1982 Aug;70(2):602–605. doi: 10.1104/pp.70.2.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jørgensen B. M., Rasmussen H. N., Rasmussen U. F. Ubiquinone reduction pattern in pigeon heart mitochondria. Identification of three distinct ubiquinone pools. Biochem J. 1985 Aug 1;229(3):621–629. doi: 10.1042/bj2290621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Landi L., Cabrini L., Sechi A. M., Pasquali P. Antioxidative effect of ubiquinones on mitochondrial membranes. Biochem J. 1984 Sep 1;222(2):463–466. doi: 10.1042/bj2220463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Millar A. H., Wiskich J. T., Whelan J., Day D. A. Organic acid activation of the alternative oxidase of plant mitochondria. FEBS Lett. 1993 Aug 30;329(3):259–262. doi: 10.1016/0014-5793(93)80233-k. [DOI] [PubMed] [Google Scholar]
  11. Ribas-Carbo M., Wiskich J. T., Berry J. A., Siedow J. N. Ubiquinone redox behavior in plant mitochondria during electron transport. Arch Biochem Biophys. 1995 Feb 20;317(1):156–160. doi: 10.1006/abbi.1995.1148. [DOI] [PubMed] [Google Scholar]
  12. Robinson S. A., Yakir D., Ribas-Carbo M., Giles L., Osmond C. B., Siedow J. N., Berry J. A. Measurements of the Engagement of Cyanide-Resistant Respiration in the Crassulacean Acid Metabolism Plant Kalanchoë daigremontiana with the Use of On-Line Oxygen Isotope Discrimination. Plant Physiol. 1992 Nov;100(3):1087–1091. doi: 10.1104/pp.100.3.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Umbach A. L., Siedow J. N. Covalent and Noncovalent Dimers of the Cyanide-Resistant Alternative Oxidase Protein in Higher Plant Mitochondria and Their Relationship to Enzyme Activity. Plant Physiol. 1993 Nov;103(3):845–854. doi: 10.1104/pp.103.3.845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Van den Bergen C. W., Wagner A. M., Krab K., Moore A. L. The relationship between electron flux and the redox poise of the quinone pool in plant mitochondria. Interplay between quinol-oxidizing and quinone-reducing pathways. Eur J Biochem. 1994 Dec 15;226(3):1071–1078. doi: 10.1111/j.1432-1033.1994.01071.x. [DOI] [PubMed] [Google Scholar]

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