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. 1995 May;108(1):337–343. doi: 10.1104/pp.108.1.337

Genetic Regulation of Development in Sorghum bicolor (IX. The ma3R Allele Disrupts Diurnal Control of Gibberellin Biosynthesis).

K R Foster 1, P W Morgan 1
PMCID: PMC157339  PMID: 12228478

Abstract

The diurnal regulation of gibberellin (GA) concentrations in Sorghum bicolor was studied in a mutant lacking a light-stable 123-kD phytochrome (ma3Rma3R), wild-type (ma3ma3,Ma3Ma3), and heterozygous (ma3ma3R) cultivars. GAs were determined in shoots of 14-d-old plants by gas chromatography-selected ion-monitoring-mass spectrometry. GA12 levels fluctuated rhythmically in Ma3Ma3, ma3ma3, and,ma3Rma3R; Peak levels occured 3 to 9 h after lights-on. In some experiments, GA53 levels followed a similar pattern. There was no rhythmicity in levels of GA19 and GA8 in any genotype. In ma3ma3 and Ma3Ma3, GA20 levels increased at lights-on, peaked in the afternoon, and decreased to minimum levels in darkness. In ma3Rma3R, peak GA20 levels occured at lights-on, 9 h earlier than in the wild-type genotypes. The pattern for GA1 levels closely followed GA20 levels in all cultivars. One copy of ma3 restored near wild-type regulation of GA20 levels. GA rhythms persisted in 25-d-old ma3ma3 plants. Since absence of the 123-kD phytochrome disrupted diurnal regulation of the GA19 -> GA20 step, the ma3Rma3R genotype may be viewed as being phase shifted in the rhythmic levels of GA20 and GA1 rather than as simply overproducing them.

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Selected References

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  1. Ballaré C. L., Scopel A. L., Sánchez R. A. Far-red radiation reflected from adjacent leaves: an early signal of competition in plant canopies. Science. 1990 Jan 19;247(4940):329–332. doi: 10.1126/science.247.4940.329. [DOI] [PubMed] [Google Scholar]
  2. Campell B. R., Bonner B. A. Evidence for Phytochrome Regulation of Gibberellin A(20) 3beta-Hydroxylation in Shoots of Dwarf (lele) Pisum sativum L. Plant Physiol. 1986 Dec;82(4):909–915. doi: 10.1104/pp.82.4.909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Childs K. L., Cordonnier-Pratt M. M., Pratt L. H., Morgan P. W. Genetic Regulation of Development in Sorghum bicolor: VII. ma(3) Flowering Mutant Lacks a Phytochrome that Predominates in Green Tissue. Plant Physiol. 1992 Jun;99(2):765–770. doi: 10.1104/pp.99.2.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Childs K. L., Pratt L. H., Morgan P. W. Genetic Regulation of Development in Sorghum bicolor: VI. The ma(3) Allele Results in Abnormal Phytochrome Physiology. Plant Physiol. 1991 Oct;97(2):714–719. doi: 10.1104/pp.97.2.714. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davies P. J., Birnberg P. R., Maki S. L., Brenner M. L. Photoperiod modification of [C]gibberellin a(12) aldehyde metabolism in shoots of pea, line g2. Plant Physiol. 1986 Aug;81(4):991–996. doi: 10.1104/pp.81.4.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Foster K. R., Miller F. R., Childs K. L., Morgan P. W. Genetic Regulation of Development in Sorghum bicolor (VIII. Shoot Growth, Tillering, Flowering, Gibberellin Biosynthesis, and Phytochrome Levels Are Differentially Affected by Dosage of the ma3R Allele. Plant Physiol. 1994 Jul;105(3):941–948. doi: 10.1104/pp.105.3.941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gianfagna T., Zeevaart J. A., Lusk W. J. Effect of photoperiod on the metabolism of deuterium-labeled gibberellin a(53) in spinach. Plant Physiol. 1983 May;72(1):86–89. doi: 10.1104/pp.72.1.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gilmour S. J., Zeevaart J. A., Schwenen L., Graebe J. E. Gibberellin metabolism in cell-free extracts from spinach leaves in relation to photoperiod. Plant Physiol. 1986 Sep;82(1):190–195. doi: 10.1104/pp.82.1.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lance B., Durley R. C., Reid D. M., Thorpe T. A., Pharis R. P. Metabolism of [H]Gibberellin A(20) in Light- and Dark-grown Tobacco Callus Cultures. Plant Physiol. 1976 Sep;58(3):387–392. doi: 10.1104/pp.58.3.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Metzger J. D., Zeevaart J. A. Effect of Photoperiod on the Levels of Endogenous Gibberellins in Spinach as Measured by Combined Gas Chromatography-selected Ion Current Monitoring. Plant Physiol. 1980 Nov;66(5):844–846. doi: 10.1104/pp.66.5.844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rood S. B., Larsen K. M., Mander L. N., Abe H., Pharis R. P. Identification of endogenous gibberellins from sorghum. Plant Physiol. 1986 Sep;82(1):330–332. doi: 10.1104/pp.82.1.330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Talon M., Zeevaart J. A., Gage D. A. Identification of Gibberellins in Spinach and Effects of Light and Darkness on their Levels. Plant Physiol. 1991 Dec;97(4):1521–1526. doi: 10.1104/pp.97.4.1521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wilson R. N., Heckman J. W., Somerville C. R. Gibberellin Is Required for Flowering in Arabidopsis thaliana under Short Days. Plant Physiol. 1992 Sep;100(1):403–408. doi: 10.1104/pp.100.1.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zeevaart J. A., Gage D. A., Talon M. Gibberellin A1 is required for stem elongation in spinach. Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7401–7405. doi: 10.1073/pnas.90.15.7401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zeevaart J. A., Gage D. A. ent-kaurene biosynthesis is enhanced by long photoperiods in the long-day plants Spinacia oleracea L. and Agrostemma githago L. Plant Physiol. 1993 Jan;101(1):25–29. doi: 10.1104/pp.101.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]

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