Abstract
In Robinia wood, the vessel-associated cells form a continuous sleeve around the vessels. Variations in pH of the solution perfused through the vessels during the annual cycle and the opposing effects of carbonyl cyanide-m-chlorophenylhydrazone and fusicoccin on this pH value indicate that some living cells of the wood are involved in the control of vascular sap pH and that this control fluctuates with the seasons. The immunolocalization of the plasma membrane HT+-ATPase in Robinia wood was studied by the immunogold-silver-staining technique using an antibody raised against a conserved stretch of the cytoplasmic domain of the H+-ATPase. The immunostaining is much stronger in vessel-associated cells than in other living cell types (ray and axial parenchyma elements) of the secondary xylem. Our data show an efficient involvement of this cell type in the control of vascular sap pH.
Full Text
The Full Text of this article is available as a PDF (1.6 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ammann D., Lanter F., Steiner R. A., Schulthess P., Shijo Y., Simon W. Neutral carrier based hydrogen ion selective microelectrode for extra- and intracellular studies. Anal Chem. 1981 Dec;53(14):2267–2269. doi: 10.1021/ac00237a031. [DOI] [PubMed] [Google Scholar]
- Bouche-Pillon S., Fleurat-Lessard P., Fromont J. C., Serrano R., Bonnemain J. L. Immunolocalization of the Plasma Membrane H+ -ATPase in Minor Veins of Vicia faba in Relation to Phloem Loading. Plant Physiol. 1994 Jun;105(2):691–697. doi: 10.1104/pp.105.2.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Katz D. B., Sussman M. R., Mierzwa R. J., Evert R. F. Cytochemical localization of ATPase activity in oat roots localizes a plasma membrane-associated soluble phosphatase, not the proton pump. Plant Physiol. 1988 Mar;86(3):841–847. doi: 10.1104/pp.86.3.841. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Li Z. S., Gallet O., Gaillard C., Lemoine R., Delrot S. The sucrose carrier of the plant plasmalemma. III. Partial purification and reconstitution of active sucrose transport in liposomes. Biochim Biophys Acta. 1992 Jan 31;1103(2):259–267. doi: 10.1016/0005-2736(92)90095-4. [DOI] [PubMed] [Google Scholar]
- Palmgren M. G., Christensen G. Functional comparisons between plant plasma membrane H(+)-ATPase isoforms expressed in yeast. J Biol Chem. 1994 Jan 28;269(4):3027–3033. [PubMed] [Google Scholar]
- Parets-Soler A., Pardo J. M., Serrano R. Immunocytolocalization of Plasma Membrane H-ATPase. Plant Physiol. 1990 Aug;93(4):1654–1658. doi: 10.1104/pp.93.4.1654. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riesmeier J. W., Hirner B., Frommer W. B. Potato sucrose transporter expression in minor veins indicates a role in phloem loading. Plant Cell. 1993 Nov;5(11):1591–1598. doi: 10.1105/tpc.5.11.1591. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Samuels A. L., Fernando M., Glass A. D. Immunofluorescent Localization of Plasma Membrane H-ATPase in Barley Roots and Effects of K Nutrition. Plant Physiol. 1992 Aug;99(4):1509–1514. doi: 10.1104/pp.99.4.1509. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sauer N., Friedländer K., Gräml-Wicke U. Primary structure, genomic organization and heterologous expression of a glucose transporter from Arabidopsis thaliana. EMBO J. 1990 Oct;9(10):3045–3050. doi: 10.1002/j.1460-2075.1990.tb07500.x. [DOI] [PMC free article] [PubMed] [Google Scholar]