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. 1995 Jul;108(3):939–947. doi: 10.1104/pp.108.3.939

A Temporarily Red Light-Insensitive Mutant of Tomato Lacks a Light-Stable, B-Like Phytochrome.

A Van Tuinen 1, LHJ Kerckhoffs 1, A Nagatani 1, R E Kendrick 1, M Koornneef 1
PMCID: PMC157443  PMID: 12228517

Abstract

We have selected four recessive mutants in tomato (Lycopersicon esculentum Mill.) that, under continuous red light (R), have long hypocotyls and small cotyledons compared to wild type (WT), a phenotype typical of phytochrome B (phyB) mutants of other species. These mutants, which are allelic, are only insensitive to R during the first 2 days upon transition from darkness to R, and therefore we propose the gene symbol tri (temporarily red light insensitive). White light-grown mutant plants have a more elongated growth habit than that of the WT. An immunochemically and spectrophotometrically detectable phyB-like polypeptide detectable in the WT is absent or below detection limits in the tri1 mutant. In contrast to the absence of an elongation growth response to far-red light (FR) given at the end of the daily photoperiod (EODFR) in all phyB-deficient mutants so far characterized, the tri1 mutant responds to EODFR treatment. The tri1 mutant also shows a strong response to supplementary daytime far-red light. We propose that the phyB-like phytochrome deficient in the tri mutants plays a major role during de-etiolation and that other light-stable phytochromes can regulate the EODFR and shade-avoidance responses in tomato.

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Selected References

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  1. Childs K. L., Cordonnier-Pratt M. M., Pratt L. H., Morgan P. W. Genetic Regulation of Development in Sorghum bicolor: VII. ma(3) Flowering Mutant Lacks a Phytochrome that Predominates in Green Tissue. Plant Physiol. 1992 Jun;99(2):765–770. doi: 10.1104/pp.99.2.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dehesh K., Franci C., Parks B. M., Seeley K. A., Short T. W., Tepperman J. M., Quail P. H. Arabidopsis HY8 locus encodes phytochrome A. Plant Cell. 1993 Sep;5(9):1081–1088. doi: 10.1105/tpc.5.9.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Devlin P. F., Rood S. B., Somers D. E., Quail P. H., Whitelam G. C. Photophysiology of the Elongated Internode (ein) Mutant of Brassica rapa: ein Mutant Lacks a Detectable Phytochrome B-Like Polypeptide. Plant Physiol. 1992 Nov;100(3):1442–1447. doi: 10.1104/pp.100.3.1442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  5. Foster K. R., Miller F. R., Childs K. L., Morgan P. W. Genetic Regulation of Development in Sorghum bicolor (VIII. Shoot Growth, Tillering, Flowering, Gibberellin Biosynthesis, and Phytochrome Levels Are Differentially Affected by Dosage of the ma3R Allele. Plant Physiol. 1994 Jul;105(3):941–948. doi: 10.1104/pp.105.3.941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Halliday K. J., Koornneef M., Whitelam G. C. Phytochrome B and at Least One Other Phytochrome Mediate the Accelerated Flowering Response of Arabidopsis thaliana L. to Low Red/Far-Red Ratio. Plant Physiol. 1994 Apr;104(4):1311–1315. doi: 10.1104/pp.104.4.1311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Inskeep W. P., Bloom P. R. Extinction coefficients of chlorophyll a and B in n,n-dimethylformamide and 80% acetone. Plant Physiol. 1985 Feb;77(2):483–485. doi: 10.1104/pp.77.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kraepiel Y., Jullien M., Cordonnier-Pratt M. M., Pratt L. Identification of two loci involved in phytochrome expression in Nicotiana plumbaginifolia and lethality of the corresponding double mutant. Mol Gen Genet. 1994 Mar;242(5):559–565. doi: 10.1007/BF00285279. [DOI] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Moran R. Formulae for determination of chlorophyllous pigments extracted with n,n-dimethylformamide. Plant Physiol. 1982 Jun;69(6):1376–1381. doi: 10.1104/pp.69.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nagatani A., Reed J. W., Chory J. Isolation and Initial Characterization of Arabidopsis Mutants That Are Deficient in Phytochrome A. Plant Physiol. 1993 May;102(1):269–277. doi: 10.1104/pp.102.1.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Parks B. M., Quail P. H. Phytochrome-Deficient hy1 and hy2 Long Hypocotyl Mutants of Arabidopsis Are Defective in Phytochrome Chromophore Biosynthesis. Plant Cell. 1991 Nov;3(11):1177–1186. doi: 10.1105/tpc.3.11.1177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reed J. W., Nagpal P., Poole D. S., Furuya M., Chory J. Mutations in the gene for the red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development. Plant Cell. 1993 Feb;5(2):147–157. doi: 10.1105/tpc.5.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sharma R., López-Juez E., Nagatani A., Furuya M. Identification of photo-inactive phytochrome A in etiolated seedlings and photo-active phytochrome B in green leaves of the aurea mutant of tomato. Plant J. 1993 Dec;4(6):1035–1042. doi: 10.1046/j.1365-313x.1993.04061035.x. [DOI] [PubMed] [Google Scholar]
  15. Whitelam G. C., Johnson E., Peng J., Carol P., Anderson M. L., Cowl J. S., Harberd N. P. Phytochrome A null mutants of Arabidopsis display a wild-type phenotype in white light. Plant Cell. 1993 Jul;5(7):757–768. doi: 10.1105/tpc.5.7.757. [DOI] [PMC free article] [PubMed] [Google Scholar]

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