Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1995 Jul;108(3):1233–1239. doi: 10.1104/pp.108.3.1233

Cold-Specific Induction of a Dehydrin Gene Family Member in Barley.

K Van Zee 1, F Q Chen 1, P M Hayes 1, T J Close 1, THH Chen 1
PMCID: PMC157478  PMID: 12228540

Abstract

An interval on barley (Hordeum vulgare L.) chromosome 7 accounting for significant quantitative trait locus effects for winter hardiness were detected in a winter (Dicktoo) x spring (Morex) barley population (P.M. Hayes, T. Blake, T.H.H. Chen, S. Tragoonrung, F. Chen, A. Pan, and B. Liu [1993] Genome 36: 66-71). Two members of the barley dehydrin gene family, Dhn1 and Dhn2, were located within the region defining the winter hardiness quantitative trait locus effect (A. Pan, P.M. Hayes, F. Chen, T. Blake, T.H.H. Chen, T.T.S. Wright, I. Karsai, Z. Bedo [1994] Theor Appl Genet 89: 900-910). To investigate the possible role of Dhn1 and Dhn2 in winter hardiness, we examined the expression pattern of six barley dehydrin gene family members in shoot tissue in response to cold temperature. Incubation of 3-week-old barley plants at 2[deg]C resulted in a rapid induction of a single 86-kD polypeptide that was recognized by an antiserum against a peptide conserved in the dehydrin gene family. Northern blot analysis confirmed the induction of an mRNA corresponding to Dhn5. The expression patterns of cold-induced dehydrins in shoot tissue for Dicktoo and Morex were identical under the conditions studied, in spite of the known phenotypic differences in their winter hardiness. These results, together with the allelic structure of selected high- and low-survival lines, suggest that the Dicktoo alleles at the Dhn1 and Dhn2 may not be the primary determinants of winter hardiness in barley.

Full Text

The Full Text of this article is available as a PDF (1.9 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allard R. W. The Wilhelmine E. Key 1987 invitational lecture. Genetic changes associated with the evolution of adaptedness in cultivated plants and their wild progenitors. J Hered. 1988 Jul-Aug;79(4):225–238. doi: 10.1093/oxfordjournals.jhered.a110503. [DOI] [PubMed] [Google Scholar]
  2. Brown R. E., Jarvis K. L., Hyland K. J. Protein measurement using bicinchoninic acid: elimination of interfering substances. Anal Biochem. 1989 Jul;180(1):136–139. doi: 10.1016/0003-2697(89)90101-2. [DOI] [PubMed] [Google Scholar]
  3. Close T. J., Fenton R. D., Moonan F. A view of plant dehydrins using antibodies specific to the carboxy terminal peptide. Plant Mol Biol. 1993 Oct;23(2):279–286. doi: 10.1007/BF00029004. [DOI] [PubMed] [Google Scholar]
  4. Close T. J., Kortt A. A., Chandler P. M. A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Mol Biol. 1989 Jul;13(1):95–108. doi: 10.1007/BF00027338. [DOI] [PubMed] [Google Scholar]
  5. Close T. J., Meyer N. C., Radik J. Nucleotide sequence of a gene encoding a 58.5-kilodalton barley dehydrin that lacks a serine tract. Plant Physiol. 1995 Jan;107(1):289–290. doi: 10.1104/pp.107.1.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guo W., Ward R. W., Thomashow M. F. Characterization of a Cold-Regulated Wheat Gene Related to Arabidopsis cor47. Plant Physiol. 1992 Oct;100(2):915–922. doi: 10.1104/pp.100.2.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hajela R. K., Horvath D. P., Gilmour S. J., Thomashow M. F. Molecular Cloning and Expression of cor (Cold-Regulated) Genes in Arabidopsis thaliana. Plant Physiol. 1990 Jul;93(3):1246–1252. doi: 10.1104/pp.93.3.1246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kurkela S., Borg-Franck M. Structure and expression of kin2, one of two cold- and ABA-induced genes of Arabidopsis thaliana. Plant Mol Biol. 1992 Jul;19(4):689–692. doi: 10.1007/BF00026794. [DOI] [PubMed] [Google Scholar]
  9. Lander E. S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S. E., Newberg L. A., Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. doi: 10.1016/0888-7543(87)90010-3. [DOI] [PubMed] [Google Scholar]
  10. Lin C., Guo W. W., Everson E., Thomashow M. F. Cold acclimation in Arabidopsis and wheat : a response associated with expression of related genes encoding ;boiling-stable' polypeptides. Plant Physiol. 1990 Nov;94(3):1078–1083. doi: 10.1104/pp.94.3.1078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Muthalif M. M., Rowland L. J. Identification of dehydrin-like proteins responsive to chilling in floral buds of blueberry (Vaccinium, section Cyanococcus). Plant Physiol. 1994 Apr;104(4):1439–1447. doi: 10.1104/pp.104.4.1439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Neven L. G., Haskell D. W., Hofig A., Li Q. B., Guy C. L. Characterization of a spinach gene responsive to low temperature and water stress. Plant Mol Biol. 1993 Jan;21(2):291–305. doi: 10.1007/BF00019945. [DOI] [PubMed] [Google Scholar]
  13. Nordin K., Heino P., Palva E. T. Separate signal pathways regulate the expression of a low-temperature-induced gene in Arabidopsis thaliana (L.) Heynh. Plant Mol Biol. 1991 Jun;16(6):1061–1071. doi: 10.1007/BF00016077. [DOI] [PubMed] [Google Scholar]
  14. Nordin K., Vahala T., Palva E. T. Differential expression of two related, low-temperature-induced genes in Arabidopsis thaliana (L.) Heynh. Plant Mol Biol. 1993 Feb;21(4):641–653. doi: 10.1007/BF00014547. [DOI] [PubMed] [Google Scholar]
  15. Robertson A. J., Weninger A., Wilen R. W., Fu P., Gusta L. V. Comparison of Dehydrin Gene Expression and Freezing Tolerance in Bromus inermis and Secale cereale Grown in Controlled Environments, Hydroponics, and the Field. Plant Physiol. 1994 Nov;106(3):1213–1216. doi: 10.1104/pp.106.3.1213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Siminovitch D., Cloutier Y. Drought and freezing tolerance and adaptation in plants: some evidence of near equivalences. Cryobiology. 1983 Aug;20(4):487–503. doi: 10.1016/0011-2240(83)90037-8. [DOI] [PubMed] [Google Scholar]
  17. Wolfraim L. A., Langis R., Tyson H., Dhindsa R. S. cDNA sequence, expression, and transcript stability of a cold acclimation-specific gene, cas18, of alfalfa (Medicago falcata) cells. Plant Physiol. 1993 Apr;101(4):1275–1282. doi: 10.1104/pp.101.4.1275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yamaguchi-Shinozaki K., Shinozaki K. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell. 1994 Feb;6(2):251–264. doi: 10.1105/tpc.6.2.251. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES