Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1995 Sep;109(1):153–159. doi: 10.1104/pp.109.1.153

Insect cell expression of recombinant imidazoleglycerolphosphate dehydratase of Arabidopsis and wheat and inhibition by triazole herbicides.

S Tada 1, M Hatano 1, Y Nakayama 1, S Volrath 1, D Guyer 1, E Ward 1, D Ohta 1
PMCID: PMC157571  PMID: 7480319

Abstract

Imidazoleglycerolphosphate dehydratase (IGPD; EC 4.2.1.19), which is involved in the histidine biosynthetic pathway of Arabidopsis thaliana and wheat (Triticum aestivum), has been expressed in insect cells using the baculovirus expression vector system. N-terminal amino acid sequencing indicated that recombinant IGPDs (rIGPDs) were produced as mature forms via nonspecific proteolytic cleavages in the putative transit peptide region. The wheat rIGPD contained one Mn atom per subunit, and the Mn was involved in the assembly of the subunits to form active IGPDs. Protein-blotting analysis, using antibodies raised against the wheat rIGPD, indicated that IGPD was located in the chloroplasts of wheat. The rIGPDs of Arabidopsis and wheat, which were 86% identical in their primary structures deduced from the cDNAs, exhibited similar properties in terms of the molecular mass, pH optimum, and the Km for the substrate, imidazoleglycerolphosphate. However, the nonselective herbicides 3-amino-1,2,4-triazole and a newly synthesized triazole [(1R*, 3R*)-[3-hydroxy-3-(2H-[1,2,4]triazole-3-yl)-cyclohexyl]- phosphonic acid], inhibited Arabidopsis and wheat IGPDs in a mixed-type and a competitive manner, respectively.

Full Text

The Full Text of this article is available as a PDF (1.9 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES B. N., MITCHELL H. K. The biosynthesis of histidine; imidazoleglycerol phosphate, imidazoleacetol phosphate, and histidinol phosphate. J Biol Chem. 1955 Feb;212(2):687–696. [PubMed] [Google Scholar]
  2. AMES B. N. The biosynthesis of histidine; D-erythro-imidazoleglycerol phosphate dehydrase. J Biol Chem. 1957 Sep;228(1):131–143. [PubMed] [Google Scholar]
  3. Andrews D. L., Beames B., Summers M. D., Park W. D. Characterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, by cloning and abundant expression in a baculovirus vector. Biochem J. 1988 May 15;252(1):199–206. doi: 10.1042/bj2520199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brady D. R., Houston L. L. Some properties of the catalytic sites of imidazoleglycerol phosphate dehydratase-histidinol phosphate phosphatase, a bifunctional enzyme from Salmonella typhimurium. J Biol Chem. 1973 Apr 10;248(7):2588–2592. [PubMed] [Google Scholar]
  5. Burns E. R., Buchanan G. A., Carter M. C. Inhibition of carotenoid synthesis as a mechanism of action of amitrole, dichlormate, and pyriclor. Plant Physiol. 1971 Jan;47(1):144–148. doi: 10.1104/pp.47.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chiariotti L., Nappo A. G., Carlomagno M. S., Bruni C. B. Gene structure in the histidine operon of Escherichia coli. Identification and nucleotide sequence of the hisB gene. Mol Gen Genet. 1986 Jan;202(1):42–47. doi: 10.1007/BF00330514. [DOI] [PubMed] [Google Scholar]
  7. Duggleby R. G. Determination of inhibition constants, I50 values and the type of inhibition for enzyme-catalyzed reactions. Biochem Med Metab Biol. 1988 Oct;40(2):204–212. doi: 10.1016/0885-4505(88)90120-x. [DOI] [PubMed] [Google Scholar]
  8. Glaser R. D., Houston L. L. Subunit structure and photooxidation of yeast imidazoleglycerolphosphate dehydratase. Biochemistry. 1974 Dec 3;13(25):5145–5152. doi: 10.1021/bi00722a015. [DOI] [PubMed] [Google Scholar]
  9. Macdonald H., Henderson J., Napier R. M., Venis M. A., Hawes C., Lazarus C. M. Authentic processing and targeting of active maize auxin-binding protein in the baculovirus expression system. Plant Physiol. 1994 Aug;105(4):1049–1057. doi: 10.1104/pp.105.4.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mano J., Hatano M., Koizumi S., Tada S., Hashimoto M., Scheidegger A. Purification and Properties of a Monofunctional Imidazoleglycerol-Phosphate Dehydratase from Wheat. Plant Physiol. 1993 Nov;103(3):733–739. doi: 10.1104/pp.103.3.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mori I., Fonne-Pfister R., Matsunaga Si., Tada S., Kimura Y., Iwasaki G., Mano Ji., Hatano M., Nakano T., Koizumi Si. A Novel Class of Herbicides (Specific Inhibitors of Imidazoleglycerol Phosphate Dehydratase). Plant Physiol. 1995 Mar;107(3):719–723. doi: 10.1104/pp.107.3.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nagai A., Suzuki K., Ward E., Moyer M., Hashimoto M., Mano J., Ohta D., Scheidegger A. Overexpression of plant histidinol dehydrogenase using a baculovirus expression vector system. Arch Biochem Biophys. 1992 Jun;295(2):235–239. doi: 10.1016/0003-9861(92)90512-u. [DOI] [PubMed] [Google Scholar]
  13. Robinson C., Ellis R. J. Transport of proteins into chloroplasts. Partial purification of a chloroplast protease involved in the processing of important precursor polypeptides. Eur J Biochem. 1984 Jul 16;142(2):337–342. doi: 10.1111/j.1432-1033.1984.tb08291.x. [DOI] [PubMed] [Google Scholar]
  14. Smith I. K. Stimulation of glutathione synthesis in photorespiring plants by catalase inhibitors. Plant Physiol. 1985 Dec;79(4):1044–1047. doi: 10.1104/pp.79.4.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Struhl K., Davis R. W. Production of a functional eukaryotic enzyme in Escherichia coli: cloning and expression of the yeast structural gene for imidazole-glycerolphosphate dehydratase (his3). Proc Natl Acad Sci U S A. 1977 Dec;74(12):5255–5259. doi: 10.1073/pnas.74.12.5255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tada S., Volrath S., Guyer D., Scheidegger A., Ryals J., Ohta D., Ward E. Isolation and characterization of cDNAs encoding imidazoleglycerolphosphate dehydratase from Arabidopsis thaliana. Plant Physiol. 1994 Jun;105(2):579–583. doi: 10.1104/pp.105.2.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wiater A., Klopotowski T., Bagdasarian G. Synergistic inhibition of plant imidazoleglycerol phosphate dehydratase by aminotriazole and phosphate. Acta Biochim Pol. 1971;18(3):309–314. [PubMed] [Google Scholar]
  18. Wiater A., Krajewska-Grynkiewicz K., Klopotowski T. Histidine biosynthesis and its regulation in higher plants. Acta Biochim Pol. 1971;18(3):299–307. [PubMed] [Google Scholar]
  19. von Heijne G., Nishikawa K. Chloroplast transit peptides. The perfect random coil? FEBS Lett. 1991 Jan 14;278(1):1–3. doi: 10.1016/0014-5793(91)80069-f. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES