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. 1995 Sep;109(1):307–317. doi: 10.1104/pp.109.1.307

Regulation of Photosynthetic Induction State in High- and Low-Light-Grown Soybean and Alocasia macrorrhiza (L.) G. Don.

J P Krall 1, E V Sheveleva 1, R W Pearcy 1
PMCID: PMC157590  PMID: 12228597

Abstract

Alocasia (Alocasia macrorrhiza [L.] G. Don) and soybean (Glycine max [L.]) were grown under high or low photon flux density (PFD) conditions to achieve a range of photosynthetic capacities and light-adaptation modes. The CO2 assimilation rate and in vivo linear electron transport rate (Jf) were determined over a range of PFDs and under saturating 1-s-duration lightflecks applied at a range of frequencies. At the same mean PFD, the assimilation rate and the Jf were lower under the lightfleck regimes than under constant light. The activation state of two, key enzymes of the photosynthetic carbon reduction cycle pathway, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and fructose-1,6-bisphosphatase, and the photosynthetic induction states (ISs) were also found to be lower under flashing as compared to continuous PFD. Under all conditions, the IS measured 120 s after an increase in PFD to constant and saturating values was highly correlated with the Rubisco activation state and stomatal conductances established in the light regime before the increase. Both the fructose-1,6-bisphosphatase and Rubisco activities established in a particular light regime were highly correlated with the mean Jf in that regime. The relationships between enzyme activation state and Jf and between IS and enzyme activation state were similar in soybean and Alocasia and were not affected either by growth-light regime, and hence photosynthetic capacity, or by flashing versus constant PFD. The common relationship between the linear Jf and the activation state of key enzymes suggests that electron transport may be the determinant of the signal regulating IS, at least to the extent that the IS is controlled by the activation state of key stromal enzymes.

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Selected References

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  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bloom A. J., Caldwell R. M., Finazzo J., Warner R. L., Weissbart J. Oxygen and carbon dioxide fluxes from barley shoots depend on nitrate assimilation. Plant Physiol. 1989 Sep;91(1):352–356. doi: 10.1104/pp.91.1.352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brooks A., Portis A. R. Protein-bound ribulose bisphosphate correlates with deactivation of ribulose bisphosphate carboxylase in leaves. Plant Physiol. 1988 May;87(1):244–249. doi: 10.1104/pp.87.1.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dujardyn M., Foyer C. H. Limitation of CO(2) Assimilation and Regulation of Benson-Calvin Cycle Activity in Barley Leaves in Response to Changes in Irradiance, Photoinhibition, and Recovery. Plant Physiol. 1989 Dec;91(4):1562–1568. doi: 10.1104/pp.91.4.1562. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harbinson J., Genty B., Foyer C. H. Relationship between Photosynthetic Electron Transport and Stromal Enzyme Activity in Pea Leaves : Toward an Understanding of the Nature of Photosynthetic Control. Plant Physiol. 1990 Oct;94(2):545–553. doi: 10.1104/pp.94.2.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hudson G. S., Evans J. R., von Caemmerer S., Arvidsson Y. B., Andrews T. J. Reduction of ribulose-1,5-bisphosphate carboxylase/oxygenase content by antisense RNA reduces photosynthesis in transgenic tobacco plants. Plant Physiol. 1992 Jan;98(1):294–302. doi: 10.1104/pp.98.1.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kirschbaum M. U., Pearcy R. W. Gas Exchange Analysis of the Fast Phase of Photosynthetic Induction in Alocasia macrorrhiza. Plant Physiol. 1988 Aug;87(4):818–821. doi: 10.1104/pp.87.4.818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kobza J., Seemann J. R. Mechanisms for light-dependent regulation of ribulose-1,5-bisphosphate carboxylase activity and photosynthesis in intact leaves. Proc Natl Acad Sci U S A. 1988 Jun;85(11):3815–3819. doi: 10.1073/pnas.85.11.3815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Peterson R. B. Effects of Irradiance on the in Vivo CO(2):O(2) Specificity Factor in Tobacco Using Simultaneous Gas Exchange and Fluorescence Techniques. Plant Physiol. 1990 Nov;94(3):892–898. doi: 10.1104/pp.94.3.892. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Robinson J. M. Carbon dioxide and nitrite photoassimilatory processes do not intercompete for reducing equivalents in spinach and soybean leaf chloroplasts. Plant Physiol. 1986 Mar;80(3):676–684. doi: 10.1104/pp.80.3.676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sassenrath-Cole G. F., Pearcy R. W. Regulation of Photosynthetic Induction State by the Magnitude and Duration of Low Light Exposure. Plant Physiol. 1994 Aug;105(4):1115–1123. doi: 10.1104/pp.105.4.1115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sassenrath-Cole G. F., Pearcy R. W. The Role of Ribulose-1,5-Bisphosphate Regeneration in the Induction Requirement of Photosynthetic CO(2) Exchange under Transient Light Conditions. Plant Physiol. 1992 May;99(1):227–234. doi: 10.1104/pp.99.1.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Seemann J. R., Kirschbaum M. U., Sharkey T. D., Pearcy R. W. Regulation of Ribulose-1,5-Bisphosphate Carboxylase Activity in Alocasia macrorrhiza in Response to Step Changes in Irradiance. Plant Physiol. 1988 Sep;88(1):148–152. doi: 10.1104/pp.88.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sharkey T. D., Seemann J. R., Pearcy R. W. Contribution of Metabolites of Photosynthesis to Postillumination CO(2) Assimilation in Response to Lightflects. Plant Physiol. 1986 Dec;82(4):1063–1068. doi: 10.1104/pp.82.4.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stitt M., Wirtz W., Heldt H. W. Metabolite levels during induction in the chloroplast and extrachloroplast compartments of spinach protoplasts. Biochim Biophys Acta. 1980 Nov 5;593(1):85–102. doi: 10.1016/0005-2728(80)90010-9. [DOI] [PubMed] [Google Scholar]
  16. Wong S. C., Cowan I. R., Farquhar G. D. Leaf Conductance in Relation to Assimilation in Eucalyptus pauciflora Sieb. ex Spreng: Influence of Irradiance and Partial Pressure of Carbon Dioxide. Plant Physiol. 1978 Oct;62(4):670–674. doi: 10.1104/pp.62.4.670. [DOI] [PMC free article] [PubMed] [Google Scholar]

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