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. 1995 Sep;109(1):319–326. doi: 10.1104/pp.109.1.319

Kinetics of NO3- Influx in Spruce.

H J Kronzucker 1, M Y Siddiqi 1, ADM Glass 1
PMCID: PMC157591  PMID: 12228598

Abstract

Influxes of 13NO3- across the root plasmalemma were measured in intact seedlings of Picea glauca (Moench) Voss. Three kinetically distinct uptake systems for NO3- were identified. In seedlings not previously exposed to external NO3-, a single Michaelis-Menten-type constitutive high-affinity transport system (CHATS) operated in a 2.5 to 500 [mu]M range of external NO3- [NO3-]o. The Vmax of this system was 0.1 [mu]mol g-1 h-1, and the Km was approximately 15 [mu]M. Following exposure to NO3- for 3 d, this CHATS activity was increased approximately 3-fold, with no change of Km. In addition, a NO3--inducible high-affinity system became apparent with a Km of approximately 100[mu]M. The combined Vmax for these discrete saturable components was 0.7 [mu]mol g-1 h-1. In both uninduced and induced plants a linear low-affinity system, additive to CHATS and an NO3--inducible high-affinity system, operated at [NO3-]o [greater than or equal to] 1 mM. The time taken to achieve maximal rates of uptake (full induction) was 2 d from 1.5 mM [NO3-]o and 3 d from 200 [mu]M [NO3-]o.

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Selected References

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  1. Aslam M., Travis R. L., Huffaker R. C. Comparative kinetics and reciprocal inhibition of nitrate and nitrite uptake in roots of uninduced and induced barley (Hordeum vulgare L.) seedlings. Plant Physiol. 1992;99:1124–1133. doi: 10.1104/pp.99.3.1124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Breteler H., Nissen P. Effect of exogenous and endogenous nitrate concentration on nitrate utilization by dwarf bean. Plant Physiol. 1982 Sep;70(3):754–759. doi: 10.1104/pp.70.3.754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DOWD J. E., RIGGS D. S. A COMPARISON OF ESTIMATES OF MICHAELIS-MENTEN KINETIC CONSTANTS FROM VARIOUS LINEAR TRANSFORMATIONS. J Biol Chem. 1965 Feb;240:863–869. [PubMed] [Google Scholar]
  4. Goyal S. S., Huffaker R. C. The uptake of NO3-, NO2-, and NH4+ by intact wheat (Triticum aestivum) seedlings. I. Induction and kinetics of transport systems. Plant Physiol. 1986;82:1051–1056. doi: 10.1104/pp.82.4.1051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hole D. J., Emran A. M., Fares Y., Drew M. C. Induction of nitrate transport in maize roots, and kinetics of influx, measured with nitrogen-13. Plant Physiol. 1990 Jun;93(2):642–647. doi: 10.1104/pp.93.2.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jackson W. A., Flesher D., Hageman R. H. Nitrate Uptake by Dark-grown Corn Seedlings: Some Characteristics of Apparent Induction. Plant Physiol. 1973 Jan;51(1):120–127. doi: 10.1104/pp.51.1.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klobus G., Ward M. R., Huffaker R. C. Characteristics of injury and recovery of net NO3- transport of barley seedlings from treatments of NaCl. Plant Physiol. 1988;87:878–882. doi: 10.1104/pp.87.4.878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Neyra C. A., Hageman R. H. Relationships between Carbon Dioxide, Malate, and Nitrate Accumulation and Reduction in Corn (Zea mays L.) Seedlings. Plant Physiol. 1976 Dec;58(6):726–730. doi: 10.1104/pp.58.6.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rao K. P., Rains D. W. Nitrate absorption by barley: I. Kinetics and energetics. Plant Physiol. 1976 Jan;57(1):55–58. doi: 10.1104/pp.57.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Serra J. L., Llama M. J., Cadenas E. Nitrate Utilization by the Diatom Skeletonema costatum: I. Kinetics of Nitrate Uptake. Plant Physiol. 1978 Dec;62(6):987–990. doi: 10.1104/pp.62.6.987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Siddiqi M. Y., Glass A. D., Ruth T. J., Fernando M. Studies of the Regulation of Nitrate Influx by Barley Seedlings Using NO(3). Plant Physiol. 1989 Jul;90(3):806–813. doi: 10.1104/pp.90.3.806. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Siddiqi M. Y., Glass A. D., Ruth T. J., Rufty T. W. Studies of the Uptake of Nitrate in Barley: I. Kinetics of NO(3) Influx. Plant Physiol. 1990 Aug;93(4):1426–1432. doi: 10.1104/pp.93.4.1426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Warner R. L., Huffaker R. C. Nitrate transport is independent of NADH and NAD(P)H nitrate reductases in barley seedlings. Plant Physiol. 1989;91:947–953. doi: 10.1104/pp.91.3.947. [DOI] [PMC free article] [PubMed] [Google Scholar]

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