Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1995 Oct;109(2):637–643. doi: 10.1104/pp.109.2.637

Effect of High Temperature on Plant Growth and Carbohydrate Metabolism in Potato.

A M Lafta 1, J H Lorenzen 1
PMCID: PMC157630  PMID: 12228617

Abstract

This study was undertaken to determine the role of sucrose-metabolizing enzymes in altered carbohydrate partitioning caused by heat stress. Potato (Solanum tuberosum L.) genotypes characterized as susceptible and tolerant to heat stress were grown at 19/17[deg]C, and a subset was transferred to 31/29[deg]C. Data were obtained for plant growth and photosynthesis. Enzyme activity was determined for sucrose-6-phosphate synthase (SPS) in mature leaves and for sucrose synthase, ADP-glucose pyrophosphorylase, and UDP-glucose pyrophosphorylase in developing tubers of plants. High temperatures reduced growth of tubers more than of shoots. Photosynthetic rates were unaffected or increased slightly at the higher temperature. Heat stress increased accumulation of foliar sucrose and decreased starch accumulation in mature leaves but did not affect glucose. SPS activity increased significantly in mature leaves of plants subjected to high temperature. Changes in SPS activity were probably not due to altered enzyme kinetics. The activity of sucrose synthase and ADP-glucose pyrophosphorylase was reduced in tubers, albeit less quickly than leaf SPS activity. There was no interaction of temperature and genotype with regard to the enzymes examined; therefore, observed differences do not account for differences between genotypes in heat susceptibility.

Full Text

The Full Text of this article is available as a PDF (693.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cheikh N., Brenner M. L. Regulation of key enzymes of sucrose biosynthesis in soybean leaves : effect of dark and light conditions and role of gibberellins and abscisic Acid. Plant Physiol. 1992 Nov;100(3):1230–1237. doi: 10.1104/pp.100.3.1230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Galtier N., Foyer C. H., Huber J., Voelker T. A., Huber S. C. Effects of Elevated Sucrose-Phosphate Synthase Activity on Photosynthesis, Assimilate Partitioning, and Growth in Tomato (Lycopersicon esculentum var UC82B). Plant Physiol. 1993 Feb;101(2):535–543. doi: 10.1104/pp.101.2.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Holaday A. S., Martindale W., Alred R., Brooks A. L., Leegood R. C. Changes in Activities of Enzymes of Carbon Metabolism in Leaves during Exposure of Plants to Low Temperature. Plant Physiol. 1992 Mar;98(3):1105–1114. doi: 10.1104/pp.98.3.1105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Huber S. C., Huber J. L. Role of sucrose-phosphate synthase in sucrose metabolism in leaves. Plant Physiol. 1992 Aug;99(4):1275–1278. doi: 10.1104/pp.99.4.1275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Huber S. C. Role of sucrose-phosphate synthase in partitioning of carbon in leaves. Plant Physiol. 1983 Apr;71(4):818–821. doi: 10.1104/pp.71.4.818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Huber S. C., Rufty T. W., Kerr P. S. Effect of Photoperiod on Photosynthate Partitioning and Diurnal Rhythms in Sucrose Phosphate Synthase Activity in Leaves of Soybean (Glycine max L. [Merr.]) and Tobacco (Nicotiana tabacum L.). Plant Physiol. 1984 Aug;75(4):1080–1084. doi: 10.1104/pp.75.4.1080. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kalt-Torres W., Kerr P. S., Usuda H., Huber S. C. Diurnal changes in maize leaf photosynthesis : I. Carbon exchange rate, assimilate export rate, and enzyme activities. Plant Physiol. 1987 Feb;83(2):283–288. doi: 10.1104/pp.83.2.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kerr P. S., Rufty T. W., Huber S. C. Endogenous Rhythms in Photosynthesis, Sucrose Phosphate Synthase Activity, and Stomatal Resistance in Leaves of Soybean (Glycine max [L.] Merr.). Plant Physiol. 1985 Feb;77(2):275–280. doi: 10.1104/pp.77.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Khayat E., Zieslin N. Effect of Night Temperature on the Activity of Sucrose Phosphate Synthase, Acid Invertase, and Sucrose Synthase in Source and Sink Tissues of Rosa hybrida cv Golden Times. Plant Physiol. 1987 Jun;84(2):447–449. doi: 10.1104/pp.84.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miron D., Schaffer A. A. Sucrose Phosphate Synthase, Sucrose Synthase, and Invertase Activities in Developing Fruit of Lycopersicon esculentum Mill. and the Sucrose Accumulating Lycopersicon hirsutum Humb. and Bonpl. Plant Physiol. 1991 Feb;95(2):623–627. doi: 10.1104/pp.95.2.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Müller-Röber B. T., Kossmann J., Hannah L. C., Willmitzer L., Sonnewald U. One of two different ADP-glucose pyrophosphorylase genes from potato responds strongly to elevated levels of sucrose. Mol Gen Genet. 1990 Oct;224(1):136–146. doi: 10.1007/BF00259460. [DOI] [PubMed] [Google Scholar]
  12. Ou-Lee T. M., Setter T. L. Enzyme activities of starch and sucrose pathways and growth of apical and Basal maize kernels. Plant Physiol. 1985 Nov;79(3):848–851. doi: 10.1104/pp.79.3.848. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pressey R. Potato sucrose synthetase: purification, properties, and changes in activity associated with maturation. Plant Physiol. 1969 May;44(5):759–764. doi: 10.1104/pp.44.5.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rufty T. W., Huber S. C. Changes in Starch Formation and Activities of Sucrose Phosphate Synthase and Cytoplasmic Fructose-1,6-bisphosphatase in Response to Source-Sink Alterations. Plant Physiol. 1983 Jun;72(2):474–480. doi: 10.1104/pp.72.2.474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sowokinos J. R. Pyrophosphorylases in Solanum tuberosum: I. Changes in ADP-Glucose and UDP-Glucose Pyrophosphorylase Activities Associated with Starch Biosynthesis during Tuberization, Maturation, and Storage of Potatoes. Plant Physiol. 1976 Jan;57(1):63–68. doi: 10.1104/pp.57.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sung S. J., Xu D. P., Black C. C. Identification of actively filling sucrose sinks. Plant Physiol. 1989 Apr;89(4):1117–1121. doi: 10.1104/pp.89.4.1117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Van Handel E. Direct microdetermination of sucrose. Anal Biochem. 1968 Feb;22(2):280–283. doi: 10.1016/0003-2697(68)90317-5. [DOI] [PubMed] [Google Scholar]
  18. Vassey T. L., Sharkey T. D. Mild Water Stress of Phaseolus vulgaris Plants Leads to Reduced Starch Synthesis and Extractable Sucrose Phosphate Synthase Activity. Plant Physiol. 1989 Apr;89(4):1066–1070. doi: 10.1104/pp.89.4.1066. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Worrell A. C., Bruneau J. M., Summerfelt K., Boersig M., Voelker T. A. Expression of a maize sucrose phosphate synthase in tomato alters leaf carbohydrate partitioning. Plant Cell. 1991 Oct;3(10):1121–1130. doi: 10.1105/tpc.3.10.1121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Xu D. P., Sung S. J., Black C. C. Sucrose metabolism in lima bean seeds. Plant Physiol. 1989 Apr;89(4):1106–1116. doi: 10.1104/pp.89.4.1106. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES