Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1995 Dec;109(4):1277–1283. doi: 10.1104/pp.109.4.1277

Plasma Membrane H+-ATPase in Maize Roots Induced for NO3- Uptake.

S Santi 1, G Locci 1, R Pinton 1, S Cesco 1, Z Varanini 1
PMCID: PMC157660  PMID: 12228668

Abstract

Plasma membrane H+-ATPase was studied in maize (Zea mays L.) roots induced for NO3- uptake. Membrane vesicles were isolated by means of Suc density gradient from roots exposed for 24 h either to 1.5 mM NO3- or 1.5 mM SO4-. The two populations of vesicles had similar composition as shown by diagnostic inhibitors of membrane-associated ATPases. However, both ATP-dependent intravesicular H+ accumulation and ATP hydrolysis were considerably enhanced (60-100%) in vesicles isolated from NO3--induced roots. Km for Mg:ATP and pH dependency were not influenced by NO3- treatment of the roots. ATP hydrolysis in plasma membrane vesicles for both control and NO3--induced roots was not affected by 10 to 150 mM NO3- or Cl-. On the other hand, kinetics of NO3-- or Cl--stimulated ATP-dependent intravesicular H+ accumulation were modified in plasma membrane vesicles isolated from NO3-- induced roots. Immunoassays carried out with polyclonal antibodies against plasma membrane H+-ATPase revealed an increased steady-state level of the enzyme in plasma membrane vesicles isolated from NO3--induced roots. Results are consistent with the idea of an involvement of plasma membrane H+-ATPase in the overall response of roots to NO3-.

Full Text

The Full Text of this article is available as a PDF (1.3 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Forbush B., 3rd Assay of Na,K-ATPase in plasma membrane preparations: increasing the permeability of membrane vesicles using sodium dodecyl sulfate buffered with bovine serum albumin. Anal Biochem. 1983 Jan;128(1):159–163. doi: 10.1016/0003-2697(83)90356-1. [DOI] [PubMed] [Google Scholar]
  3. Gallagher S. R., Leonard R. T. Electrophoretic characterization of a detergent-treated plasma membrane fraction from corn roots. Plant Physiol. 1987 Feb;83(2):265–271. doi: 10.1104/pp.83.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Giannini J. L., Briskin D. P. Proton Transport in Plasma Membrane and Tonoplast Vesicles from Red Beet (Beta vulgaris L.) Storage Tissue : A Comparative Study of Ion Effects on DeltapH and DeltaPsi. Plant Physiol. 1987 Jul;84(3):613–618. doi: 10.1104/pp.84.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldsmith J., Livoni J. P., Norberg C. L., Segel I. H. Regulation of Nitrate Uptake in Penicillium chrysogenum by Ammonium Ion. Plant Physiol. 1973 Oct;52(4):362–367. doi: 10.1104/pp.52.4.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Harper J. F., Manney L., DeWitt N. D., Yoo M. H., Sussman M. R. The Arabidopsis thaliana plasma membrane H(+)-ATPase multigene family. Genomic sequence and expression of a third isoform. J Biol Chem. 1990 Aug 15;265(23):13601–13608. [PubMed] [Google Scholar]
  7. Hole D. J., Emran A. M., Fares Y., Drew M. C. Induction of nitrate transport in maize roots, and kinetics of influx, measured with nitrogen-13. Plant Physiol. 1990 Jun;93(2):642–647. doi: 10.1104/pp.93.2.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. King B. J., Siddiqi M. Y., Glass A. D. Studies of the uptake of nitrate in barley : v. Estimation of root cytoplasmic nitrate concentration using nitrate reductase activity-implications for nitrate influx. Plant Physiol. 1992 Aug;99(4):1582–1589. doi: 10.1104/pp.99.4.1582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Lew R. R., Spanswick R. M. Characterization of Anion Effects on the Nitrate-Sensitive ATP-Dependent Proton Pumping Activity of Soybean (Glycine max L.) Seedling Root Microsomes. Plant Physiol. 1985 Feb;77(2):352–357. doi: 10.1104/pp.77.2.352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McClure P. R., Kochian L. V., Spanswick R. M., Shaff J. E. Evidence for Cotransport of Nitrate and Protons in Maize Roots : II. Measurement of NO(3) and H Fluxes with Ion-Selective Microelectrodes. Plant Physiol. 1990 May;93(1):290–294. doi: 10.1104/pp.93.1.290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Michelet B., Lukaszewicz M., Dupriez V., Boutry M. A plant plasma membrane proton-ATPase gene is regulated by development and environment and shows signs of a translational regulation. Plant Cell. 1994 Oct;6(10):1375–1389. doi: 10.1105/tpc.6.10.1375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Oakley B. R., Kirsch D. R., Morris N. R. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980 Jul 1;105(2):361–363. doi: 10.1016/0003-2697(80)90470-4. [DOI] [PubMed] [Google Scholar]
  14. Palmgren M. G. Acridine orange as a probe for measuring pH gradients across membranes: mechanism and limitations. Anal Biochem. 1991 Feb 1;192(2):316–321. doi: 10.1016/0003-2697(91)90542-2. [DOI] [PubMed] [Google Scholar]
  15. Palmgren M. G., Christensen G. Functional comparisons between plant plasma membrane H(+)-ATPase isoforms expressed in yeast. J Biol Chem. 1994 Jan 28;269(4):3027–3033. [PubMed] [Google Scholar]
  16. Palmgren M. G., Larsson C., Sommarin M. Proteolytic activation of the plant plasma membrane H(+)-ATPase by removal of a terminal segment. J Biol Chem. 1990 Aug 15;265(23):13423–13426. [PubMed] [Google Scholar]
  17. Ruiz-Cristin J., Briskin D. P. Characterization of a H+/NO3- symport associated with plasma membrane vesicles of maize roots using 36ClO3- as a radiotracer analog. Arch Biochem Biophys. 1991 Feb 15;285(1):74–82. doi: 10.1016/0003-9861(91)90330-l. [DOI] [PubMed] [Google Scholar]
  18. Samuels A. L., Fernando M., Glass A. D. Immunofluorescent Localization of Plasma Membrane H-ATPase in Barley Roots and Effects of K Nutrition. Plant Physiol. 1992 Aug;99(4):1509–1514. doi: 10.1104/pp.99.4.1509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Siddiqi M. Y., Glass A. D., Ruth T. J., Rufty T. W. Studies of the Uptake of Nitrate in Barley: I. Kinetics of NO(3) Influx. Plant Physiol. 1990 Aug;93(4):1426–1432. doi: 10.1104/pp.93.4.1426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. de Boer A. H., Watson B. A., Cleland R. E. Purification and identification of the fusicoccin binding protein from oat root plasma membrane. Plant Physiol. 1989;89:250–259. doi: 10.1104/pp.89.1.250. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES