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. 1995 Dec;109(4):1285–1293. doi: 10.1104/pp.109.4.1285

Sucrose Synthase Localization during Initiation of Seed Development and Trichome Differentiation in Cotton Ovules.

K D Nolte 1, D L Hendrix 1, J W Radin 1, K E Koch 1
PMCID: PMC157661  PMID: 12228669

Abstract

Sucrose synthase in cotton (Gossypium hirsutum L.) ovules was immunolocalized to clarify the relationship between this enzyme and (a) sucrose import/utilization during initiation of seed development, (b) trichome differentiation, and (c) cell-wall biosynthesis in these rapidly elongating "fibers." Analyses focused on the period immediately before and after trichome initiation (at pollination). Internal tissues most heavily immunolabeled were the developing nucellus, adjacent integument (inner surface), and the vascular region. Little sucrose synthase was associated with the outermost epidermis on the day preceding pollination. However, 1 d later, immunolabel appeared specifically in those epidermal cells at the earliest visible phase of trichome differentiation. The day following pollination, these cells had elongated 3- to 5-fold and showed a further enhancement of sucrose synthase immunolabel. Levels of sucrose synthase mRNA also increased during this period, regardless of whether pollination per se had occurred. Timing of onset for the cell-specific localization of sucrose synthase in young seeds and trichome initials indicates a close association between this enzyme and sucrose import at a cellular level, as well as a potentially integral role in cell-wall biosynthesis.

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Selected References

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  1. Koch K. E., Nolte K. D., Duke E. R., McCarty D. R., Avigne W. T. Sugar Levels Modulate Differential Expression of Maize Sucrose Synthase Genes. Plant Cell. 1992 Jan;4(1):59–69. doi: 10.1105/tpc.4.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Maas C., Schaal S., Werr W. A feedback control element near the transcription start site of the maize Shrunken gene determines promoter activity. EMBO J. 1990 Nov;9(11):3447–3452. doi: 10.1002/j.1460-2075.1990.tb07552.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Morrow D. L., Lucas W. J. (1-->3)-beta-d-Glucan Synthase from Sugar Beet : II. Product Inhibition by UDP. Plant Physiol. 1987 Jul;84(3):565–567. doi: 10.1104/pp.84.3.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Nolte K. D., Koch K. E. Companion-Cell Specific Localization of Sucrose Synthase in Zones of Phloem Loading and Unloading. Plant Physiol. 1993 Mar;101(3):899–905. doi: 10.1104/pp.101.3.899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Salanoubat M., Belliard G. The steady-state level of potato sucrose synthase mRNA is dependent on wounding, anaerobiosis and sucrose concentration. Gene. 1989 Dec 7;84(1):181–185. doi: 10.1016/0378-1119(89)90153-4. [DOI] [PubMed] [Google Scholar]
  6. Schulz P., Jensen W. A. Capsella embryogenesis: the chalazal proliferating tissue. J Cell Sci. 1971 Jan;8(1):201–227. doi: 10.1242/jcs.8.1.201. [DOI] [PubMed] [Google Scholar]
  7. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tomlinson P. T., Duke E. R., Nolte K. D., Koch K. E. Sucrose synthase and invertase in isolated vascular bundles. Plant Physiol. 1991 Nov;97(3):1249–1252. doi: 10.1104/pp.97.3.1249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wang F., Smith A. G., Brenner M. L. Temporal and Spatial Expression Pattern of Sucrose Synthase during Tomato Fruit Development. Plant Physiol. 1994 Feb;104(2):535–540. doi: 10.1104/pp.104.2.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Woodson W. R., Park K. Y., Drory A., Larsen P. B., Wang H. Expression of ethylene biosynthetic pathway transcripts in senescing carnation flowers. Plant Physiol. 1992 Jun;99(2):526–532. doi: 10.1104/pp.99.2.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Xu D. P., Sung S. J., Black C. C. Sucrose metabolism in lima bean seeds. Plant Physiol. 1989 Apr;89(4):1106–1116. doi: 10.1104/pp.89.4.1106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zhang X. S., O'Neill S. D. Ovary and Gametophyte Development Are Coordinately Regulated by Auxin and Ethylene following Pollination. Plant Cell. 1993 Apr;5(4):403–418. doi: 10.1105/tpc.5.4.403. [DOI] [PMC free article] [PubMed] [Google Scholar]

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