Skip to main content
The Canadian Veterinary Journal logoLink to The Canadian Veterinary Journal
. 1997 Jul;38(7):429–437.

Streptococcus agalactiae mastitis: a review.

G P Keefe 1
PMCID: PMC1576741  PMID: 9220132

Abstract

Streptococcus agalactiae continues to be a major cause of subclinical mastitis in dairy cattle and a source of economic loss for the industry. Veterinarians are often asked to provide information on herd level control and eradication of S. agalactiae mastitis. This review collects and collates relevant publications on the subject. The literature search was conducted in 1993 on the Agricola database. Articles related to S. agalactiae epidemiology, pathogen identification techniques, milk quality consequences, and control, prevention, and therapy were included. Streptococcus agalactiae is an oblique parasite of the bovine mammary gland and is susceptible to treatment with a variety of antibiotics. Despite this fact, where state or provincial census data are available, herd prevalence levels range from 11% (Alberta, 1991) to 47% (Vermont, 1985). Infection with S. agalactiae is associated with elevated somatic cell count and total bacteria count and a decrease in the quantity and quality of milk products produced. Bulk tank milk culture has, using traditional milk culture techniques, had a low sensitivity for identifying S. agalactiae at the herd level. New culture methods, using selective media and large inocula, have substantially improved the sensitivity of bulk tank culture. Efficacy of therapy on individual cows remains high. Protocols for therapy of all infected animals in a herd are generally successful in eradicating the pathogen from the herd, especially if they are followed up with good udder hygiene techniques.

Full text

PDF
429

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agger J. F., Priou C., Huda A., Aagaard K. Risk factors for transmission of Streptococcus agalactiae infection between Danish dairy herds: a case control study. Vet Res. 1994;25(2-3):227–234. [PubMed] [Google Scholar]
  2. Ahl A. S., Gibson C. D., Kirk J. H., Kaneene J. B., Ahl J. G. Cost of mastitis and its prevention in four dairy cattle herds on St Croix, US Virgin Islands. J Am Vet Med Assoc. 1989 May 15;194(10):1418–1421. [PubMed] [Google Scholar]
  3. Ainsworth A. J., Capley G. Monoclonal antibodies produced to Streptococcus agalactiae. Am J Vet Res. 1986 Jun;47(6):1211–1213. [PubMed] [Google Scholar]
  4. Bartlett P. C., Miller G. Y., Lance S. E., Hancock D. D., Heider L. E. Managerial risk factors of intramammary infection with Streptococcus agalactiae in dairy herds in Ohio. Am J Vet Res. 1992 Sep;53(9):1715–1721. [PubMed] [Google Scholar]
  5. Blosser T. H. Economic losses from and the national research program on mastitis in the United States. J Dairy Sci. 1979 Jan;62(1):119–127. doi: 10.3168/jds.S0022-0302(79)83213-0. [DOI] [PubMed] [Google Scholar]
  6. Brown J., Farnsworth R., Wannamaker L. W., Johnson D. W. CAMP factor of group B streptococci: production, assay, and neutralization by sera from immunized rabbits and experimentally infected cows. Infect Immun. 1974 Feb;9(2):377–383. doi: 10.1128/iai.9.2.377-383.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Brown M. B., Scasserra A. E. Antimicrobial resistance in streptococcal species isolated from bovine mammary glands. Am J Vet Res. 1990 Dec;51(12):2015–2018. [PubMed] [Google Scholar]
  8. Craven N. Efficacy and financial value of antibiotic treatment of bovine clinical mastitis during lactation--a review. Br Vet J. 1987 Sep-Oct;143(5):410–422. doi: 10.1016/0007-1935(87)90018-2. [DOI] [PubMed] [Google Scholar]
  9. De La Rosa M., Villareal R., Vega D., Miranda C., Martinezbrocal A. Granada medium for detection and identification of group B streptococci. J Clin Microbiol. 1983 Oct;18(4):779–785. doi: 10.1128/jcm.18.4.779-785.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dinsmore R. P., English P. B., Gonzalez R. N., Sears P. M., Schulte H. F. Evaluation of methods for the diagnosis of Streptococcus agalactiae intramammary infections in dairy cattle. J Dairy Sci. 1991 May;74(5):1521–1526. doi: 10.3168/jds.S0022-0302(91)78312-4. [DOI] [PubMed] [Google Scholar]
  11. Dinsmore R. P., English P. B., Gonzalez R. N., Sears P. M. Use of augmented cultural techniques in the diagnosis of the bacterial cause of clinical bovine mastitis. J Dairy Sci. 1992 Oct;75(10):2706–2712. doi: 10.3168/jds.s0022-0302(92)78032-1. [DOI] [PubMed] [Google Scholar]
  12. Dodd F. H. Mastitis--progress on control. J Dairy Sci. 1983 Aug;66(8):1773–1780. doi: 10.3168/jds.S0022-0302(83)82005-0. [DOI] [PubMed] [Google Scholar]
  13. Edmondson P. W. An economic justification of "blitz" therapy to eradicate Streptococcus agalactiae from a dairy herd. Vet Rec. 1989 Dec 9;125(24):591–593. [PubMed] [Google Scholar]
  14. Erskine R. J., Eberhart R. J. Herd benefit-to-cost ratio and effects of a bovine mastitis control program that includes blitz treatment of Streptococcus agalactiae. J Am Vet Med Assoc. 1990 Apr 15;196(8):1230–1235. [PubMed] [Google Scholar]
  15. Erskine R. J., Eberhart R. J., Hutchinson L. J., Spencer S. B. Herd management and prevalence of mastitis in dairy herds with high and low somatic cell counts. J Am Vet Med Assoc. 1987 Jun 1;190(11):1411–1416. [PubMed] [Google Scholar]
  16. Facklam R. R., Cooksey R. C., Wortham E. C. Evaluation of commercial latex agglutination reagents for grouping streptococci. J Clin Microbiol. 1979 Nov;10(5):641–646. doi: 10.1128/jcm.10.5.641-646.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Fallon R. J. The rapid recognition of Lancefield group B haemolytic streptococci. J Clin Pathol. 1974 Nov;27(11):902–905. doi: 10.1136/jcp.27.11.902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Francis P. G. Update on mastitis. III. Mastitis therapy. Br Vet J. 1989 Jul-Aug;145(4):302–311. doi: 10.1016/0007-1935(89)90027-4. [DOI] [PubMed] [Google Scholar]
  19. Gonzalez R. N., Jasper D. E., Bushnell R. B., Farver T. B. Relationship between mastitis pathogen numbers in bulk tank milk and bovine udder infections in California dairy herds. J Am Vet Med Assoc. 1986 Aug 15;189(4):442–445. [PubMed] [Google Scholar]
  20. González R. N., Jasper D. E., Farver T. B., Bushnell R. B., Franti C. E. Prevalence of udder infections and mastitis in 50 California dairy herds. J Am Vet Med Assoc. 1988 Aug 1;193(3):323–328. [PubMed] [Google Scholar]
  21. Goodger W. J., Ruppanner R. Why the dairy industry does not make greater use of veterinarians. J Am Vet Med Assoc. 1982 Oct 1;181(7):706–710. [PubMed] [Google Scholar]
  22. Greer D. O., Pearson J. K. Streptococcus agalactiae in dairy heards. Its incidence and relationship to cell count and inhibitory substance levels in bulk milk. Br Vet J. 1973 Dec;129(6):544–554. doi: 10.1016/s0007-1935(17)36337-6. [DOI] [PubMed] [Google Scholar]
  23. Guterbock W. M., Blackmer P. E. Veterinary interpretation of bulk-tank milk. Vet Clin North Am Large Anim Pract. 1984 Jul;6(2):257–268. doi: 10.1016/s0196-9846(17)30021-6. [DOI] [PubMed] [Google Scholar]
  24. Hogan J. S., Smith K. L., Todhunter D. A., Schoenberger P. S. Sensitivity and specificity of latex agglutination tests used to identify Streptococcus agalactiae and Staphylococcus aureus isolated from bulk tank milk. Am J Vet Res. 1988 Sep;49(9):1537–1539. [PubMed] [Google Scholar]
  25. Huber W. G. Antibacterial drug effectiveness against mastitis pathogens. J Am Vet Med Assoc. 1977 May 15;170(10 Pt 2):1182–1184. [PubMed] [Google Scholar]
  26. Jain N. C. Common mammary pathogens and factors in infection and mastitis. J Dairy Sci. 1979 Jan;62(1):128–134. doi: 10.3168/jds.S0022-0302(79)83214-2. [DOI] [PubMed] [Google Scholar]
  27. Janzen J. J. Economic losses resulting from mastitis. A review. J Dairy Sci. 1970 Sep;53(9):1151–1161. doi: 10.3168/jds.S0022-0302(70)86361-5. [DOI] [PubMed] [Google Scholar]
  28. Jasper D. E., Dellinger J. D., Bushnell R. R. Agreement of duplicate samples of milk for the evaluation of quarter infection. Am J Vet Res. 1974 Oct;35(10):1371–1373. [PubMed] [Google Scholar]
  29. Kingwill R. G., Neave F. K., Dodd F. H., Griffin T. K., Westgarth D. R., Wilson C. D. The effect of a mastitis control system on levels of subclinical and clinical mastitis in two years. Vet Rec. 1970 Jul 25;87(4):94–100. doi: 10.1136/vr.87.4.94. [DOI] [PubMed] [Google Scholar]
  30. McDonald J. S., McDonald T. J., Stark D. R. Antibiograms of streptococci isolated from bovine intramammary infections. Am J Vet Res. 1976 Oct;37(10):1185–1188. [PubMed] [Google Scholar]
  31. McDonald J. S. Streptococcal and staphylococcal mastitis. J Am Vet Med Assoc. 1977 May 15;170(10 Pt 2):1157–1159. [PubMed] [Google Scholar]
  32. Merritt K., Jacobs N. J. Characterization and incidence of pigment production by human clinical group B streptococci. J Clin Microbiol. 1978 Jul;8(1):105–107. doi: 10.1128/jcm.8.1.105-107.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Mhalu F. S. Infection with Streptococcus agalactiae in a London hospital. J Clin Pathol. 1976 Apr;29(4):309–312. doi: 10.1136/jcp.29.4.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Natzke R. P., Everett R. W., Bray D. R. Effect of drying off practices on mastitis infection. J Dairy Sci. 1975 Dec;58(12):1828–1835. doi: 10.3168/jds.S0022-0302(75)84794-1. [DOI] [PubMed] [Google Scholar]
  35. Oliver S. P., Mitchell B. A. Prevalence of mastitis pathogens in herds participating in a mastitis control program. J Dairy Sci. 1984 Oct;67(10):2436–2440. doi: 10.3168/jds.S0022-0302(84)81592-1. [DOI] [PubMed] [Google Scholar]
  36. Pankey J. W. Hygiene at milking time in the prevention of bovine mastitis. Br Vet J. 1989 Sep-Oct;145(5):401–409. doi: 10.1016/0007-1935(89)90047-X. [DOI] [PubMed] [Google Scholar]
  37. Pankey J. W. Premilking udder hygiene. J Dairy Sci. 1989 May;72(5):1308–1312. doi: 10.3168/jds.S0022-0302(89)79238-9. [DOI] [PubMed] [Google Scholar]
  38. Philpot W. N. Control of mastitis by hygiene and therapy. J Dairy Sci. 1979 Jan;62(1):168–176. doi: 10.3168/jds.S0022-0302(79)83218-X. [DOI] [PubMed] [Google Scholar]
  39. Poutrel B. Comparative evaluation of commercial latex agglutination and coagglutination reagents for groups B, C, and D mastitis streptococci. Am J Vet Res. 1983 Mar;44(3):490–492. [PubMed] [Google Scholar]
  40. Reneau J. K. Effective use of dairy herd improvement somatic cell counts in mastitis control. J Dairy Sci. 1986 Jun;69(6):1708–1720. doi: 10.3168/jds.S0022-0302(86)80590-2. [DOI] [PubMed] [Google Scholar]
  41. Schukken Y. H., Grommers F. J., Smit J. A., Vandegeer D., Brand A. Effect of freezing on bacteriologic culturing of mastitis milk samples. J Dairy Sci. 1989 Jul;72(7):1900–1906. doi: 10.3168/jds.S0022-0302(89)79309-7. [DOI] [PubMed] [Google Scholar]
  42. Sischo W. M., Heider L. E., Miller G. Y., Moore D. A. Prevalence of contagious pathogens of bovine mastitis and use of mastitis control practices. J Am Vet Med Assoc. 1993 Feb 15;202(4):595–600. [PubMed] [Google Scholar]
  43. Slifkin M., Engwall C., Pouchet G. R. Direct-plate serological grouping of beta-hemolytic streptococci from primary isolation plates with the Phadebact streptococcus test. J Clin Microbiol. 1978 Apr;7(4):356–360. doi: 10.1128/jcm.7.4.356-360.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Smith A. R., Ward G. E. Evaluation of methods for control of Streptococcus agalactiae in dairy herds. Can Vet J. 1975 Apr;16(4):109–113. [PMC free article] [PubMed] [Google Scholar]
  45. Thomson J. R., Mollison N., Matthews K. P. An investigation of mastitis due to S agalactiae, S uberis and M smegmatis in a dairy herd. Vet Rec. 1988 Mar 19;122(12):271–274. doi: 10.1136/vr.122.12.271. [DOI] [PubMed] [Google Scholar]
  46. Thurmond M. C., Tyler J. W., Luiz D. M., Holmberg C. A., Picanso J. P. The effect of pre-enrichment on recovery of Streptococcus agalactiae, Staphylococcus aureus and mycoplasma from bovine milk. Epidemiol Infect. 1989 Dec;103(3):465–474. doi: 10.1017/s0950268800030879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Tyler J. W., Wilson R. C., Dowling P. Treatment of subclinical mastitis. Vet Clin North Am Food Anim Pract. 1992 Mar;8(1):17–28. doi: 10.1016/s0749-0720(15)30758-1. [DOI] [PubMed] [Google Scholar]
  48. Villanueva M. R., Tyler J. W., Thurmond M. C. Recovery of Streptococcus agalactiae and Staphylococcus aureus from fresh and frozen bovine milk. J Am Vet Med Assoc. 1991 Apr 15;198(8):1398–1400. [PubMed] [Google Scholar]
  49. Wanasinghe D. D., Frost A. J. The prevalence of udder infection and mastitis in herds producing bulk milk with either consistently high or low cell count. Aust Vet J. 1979 Aug;55(8):374–380. [PubMed] [Google Scholar]
  50. Wanger A. R., Dunny G. M. Development of a system for genetic and molecular analysis of Streptococcus agalactiae. Res Vet Sci. 1985 Mar;38(2):202–208. [PubMed] [Google Scholar]
  51. Wanger A. R., Dunny G. M. Identification of a Streptococcus agalactiae protein antigen associated with bovine mastitis isolates. Infect Immun. 1987 May;55(5):1170–1175. doi: 10.1128/iai.55.5.1170-1175.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  52. Wanger A. R., Dunny G. M. Specific agglutination of Streptococcus agalactiae from bovine mastitis by casein components of bovine milk. J Dairy Sci. 1984 Oct;67(10):2441–2445. doi: 10.3168/jds.S0022-0302(84)81593-3. [DOI] [PubMed] [Google Scholar]
  53. Ward G. E., Schultz L. H. Relationship of somatic cells in quarter milk to type of bacteria and production. J Dairy Sci. 1972 Oct;55(10):1428–1431. doi: 10.3168/jds.S0022-0302(72)85689-3. [DOI] [PubMed] [Google Scholar]
  54. Watts J. L., Owens W. E. Evaluation of the Rapid Mastitis Test for identification of Staphylococcus aureus and Streptococcus agalactiae isolated from bovine mammary glands. J Clin Microbiol. 1988 Apr;26(4):672–674. doi: 10.1128/jcm.26.4.672-674.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  55. Weaver L. D., Galland J., Martin P. A., Versteeg J. Treatment of Streptococcus agalactiae mastitis in dairy cows: comparative efficacies of two antibiotic preparations and factors associated with successful treatment. J Am Vet Med Assoc. 1986 Sep 15;189(6):666–669. [PubMed] [Google Scholar]
  56. Wise J. K. US market for food animal veterinary medical services. J Am Vet Med Assoc. 1987 Jun 15;190(12):1530–1533. [PubMed] [Google Scholar]
  57. Yamagata M., Goodger W. J., Weaver L., Franti C. The economic benefit of treating subclinical Streptococcus agalactiae mastitis in lactating cows. J Am Vet Med Assoc. 1987 Dec 15;191(12):1556–1561. [PubMed] [Google Scholar]

Articles from The Canadian Veterinary Journal are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES